Resonance scattering spectral detection of trace ATP based on label-free aptamer reaction and nanogold catalysis

Abstract

Double-stranded DNA (dsDNA) cannot protect gold nanoparticles (AuNPs) in the presence of NaCl, and dsDNA interacted with adenosine triphosphate (ATP) to form stable G-quartet and a single-stranded DNA (DNA 2) that can protect AuNPs. The unprotected AuNPs were aggregated to AuNP aggregations (AuNPA) that exhibited a resonance scattering (RS) peak at 590 nm. The RS intensity at 590 nm decreased linearly when the ATP concentration increased in the range of 6.6-110 nM. The catalysis of AuNP-DNA 2 was stronger than that of the AuNPA on the glucose-Cu(II) particle reaction, and the product appeared as an RS peak at 620 nm. When the ATP concentration was increased, the AuNP-DNA 2 increased, and the RS intensity at 620 nm increased linearly. The increased RS intensity (ΔI(620 nm)) was linear to ATP concentration in the range of 2.2-220 nM, with a regression equation of ΔI(620 nm) = 0.709C + 7.7, and a detection limit of 0.5 nM. Hereby, a new RS method of ATP detection was set up with high sensitivity and selectivity.

A simple and sensitive resonance Rayleigh scattering method for determination of As(III) using aptamer‐modified nanogold as a probe

“…Aptamers are single‐stranded oligonucleotide fragments that consist of 10 or more . With high specificity, high stability, simple synthesis and modification in vitro , aptamer can specifically bind the target substances and has been used for analysis and clinical testing . Resonance Rayleigh scattering (RRS) spectral method is simple, rapid and sensitive, and it has been applied to the analysis of nucleic acids, proteins and small molecules .…”

“…Resonance Rayleigh scattering (RRS) spectral method is simple, rapid and sensitive, and it has been applied to the analysis of nucleic acids, proteins and small molecules . Nanogold, with good stability, good biocompatibility and RRS effect, has been applied in the aptamer–RRS analysis . Jiang et al .’s research group has reported an aptamer‐modified nanosilver RRS method for the detection of 0.02–1.06 µg/L melamine .…”

A label-free colorimetric progesterone aptasensor based on the aggregation of gold nanoparticles

“…Finally, the cooled solution was filtered through 0.2 μm ultrafiltration membranes, and then stored in a brown glass bottles at 4°C for further use. Besides, the concentration of AuNPs, as a measure of the concentration of Au, was calculated as 7.3 × 10 −4 M [26].…”

Resonance light scattering determination of trace bisphenol A with signal amplification by aptamer–nanogold catalysis

“…An aptamer is a short single‐stranded oligonucleotide containing 20–100 bases that can specifically recognize proteins, polypeptides, organic compounds, metal ions, various ligands, etc. It has been combined with RLS and a nano‐labeled technique to develop simple, sensitive and selective aptamer‐modified nanoparticle RLS methods for trace metal ions, melamine, ATP, adenosine and thrombin . To our knowledge, there is no report of an aptamer–RLS method for detecting the environmental estrogen bisphenol A (BPA).…”