Resolving Rotational Motions of Nano-objects in Engineered Environments and Live Cells with Gold Nanorods and Differential Interference Contrast Microscopy

Wang, Gufeng; Sun, Wei; Luo, Yong; Fang, Ning

doi:10.1021/ja106506k

Cited by 159 publications

(244 citation statements)

References 39 publications

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“…The cpp was necessary to allow cell penetration and consequent investigation of the possible intracellular function of these peptides. Moreover, the cpp used herein has been extensively described to transport its cargo into the cells (37,38).…”

Section: Resultsmentioning

confidence: 99%

A Novel Intracellular Peptide Derived from G1/S Cyclin D2 Induces Cell Death

Araujo

Russo

Castro

et al. 2014

Journal of Biological Chemistry

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Background: Intracellular peptides probably regulate several biological processes. Results: pep5 derived from G 1 /S cyclin D2 specifically increases during the S phase of the cell cycle and, reintroduced into the cell, induces apoptosis and necrosis. Conclusion: pep5 has potential therapeutic applications and could have biological functions. Significance: pep5 discovery advances our understanding of limited proteolysis.

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Section: Resultsmentioning

confidence: 99%

A Novel Intracellular Peptide Derived from G1/S Cyclin D2 Induces Cell Death

Araujo

Russo

Castro

et al. 2014

Journal of Biological Chemistry

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“…Recently, we introduced the single particle orientation and rotational tracking (SPORT) technique [34][35][36] to follow the rotational motion of plasmonic gold nanorods 37 under a differential interference contrast (DIC) microscope. Working in the principle of interferometry 38 , a DIC microscope generates disproportionate bright and dark interference patterns for gold nanorods in different orientations.…”

mentioning

confidence: 99%

“…By measuring the bright and dark DIC intensities of the gold nanorod continuously, it becomes possible to dynamically track the nanorod's orientation and rotational patterns. Using the SPORT technique with an electron-multiplying charge-coupled device running at a temporal resolution of 30 ms, we observed that a cargo tends to keep the same orientation relative to the microtubule tracks during the active transport in living cells 34 .…”

mentioning

confidence: 99%

Rotational dynamics of cargos at pauses during axonal transport

Sun

Wang

et al. 2012

Nat Commun

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Direct visualization of axonal transport in live neurons is essential for our understanding of the neuronal functions and the working mechanisms of microtubule-based motor proteins. Here we use the high-speed single particle orientation and rotational tracking technique to directly visualize the rotational dynamics of cargos in both active directional transport and pausing stages of axonal transport, with a temporal resolution of 2 ms. Both long and short pauses are imaged, and the correlations between the pause duration, the rotational behaviour of the cargo at the pause, and the moving direction after the pause are established. Furthermore, the rotational dynamics leading to switching tracks are visualized in detail. These first-time observations of cargo's rotational dynamics provide new insights on how kinesin and dynein motors take the cargo through the alternating stages of active directional transport and pause.

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“…This is in good agreement with the polarizationdependent rotation behaviors of plasmonic nanoparticles that have been extensively investigated in gold nanorods and nanowires. 18,39 In view of the periodic changes, a correlation between the bright and dark intensity traces is observed: the bright and dark intensities increase and decrease in the same direction. This orientation/polarization dependence is the foundation for the use of Au/Ag/SiO 2 −NRs as SPORT probes.…”

Section: * S Supporting Informationmentioning

confidence: 92%

“…18,38 Herein, the DIC contrast of an immobilized, randomly oriented Au/Ag/SiO 2 −NR is plotted as a function of excitation wavelength ( Figure S3 in the Supporting Information). The highest DIC contrast for this particle was found when a 585 ± 29 nm filter was used for illumination, which corresponds to the longitudinal dipolar plasmonic band.…”

Section: * S Supporting Informationmentioning

confidence: 99%

Multishell Au/Ag/SiO₂ Nanorods with Tunable Optical Properties as Single Particle Orientation and Rotational Tracking Probes

et al. 2015

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Three-layer core-shell plasmonic nanorods (Au/Ag/SiO 2-NRs), consisting of a gold nanorod core, a thin silver shell, and a thin silica layer, were synthesized and used as optical imaging probes under a differential interference contrast microscope for single particle orientation and rotational tracking. The localized surface plasmon resonance modes were enhanced upon the addition of the silver shell, and the anisotropic optical properties of gold nanorods were maintained. The silica coating enables surface functionalization with silane coupling agents and provides enhanced stability and biocompatibility. Taking advantage of the longitudinal LSPR enhancement, the orientation and rotational information of the hybrid nanorods on synthetic lipid bilayers and on live cell membranes were obtained with millisecond temporal resolution using a scientific complementary metal-oxide-semiconductor camera. The results demonstrate that the as-synthesized hybrid nanorods are promising imaging probes with improved sensitivity and good biocompatibility for single plasmonic particle tracking experiments in biological systems.Keywords biocompatibility, cell membranes, coupling agents, cytology, image processing, lipid bilayers, MOS devices, nanorods, optical properties, plasmons, probes, silanes, silica, silver, surface plasmon resonance, anisotropic optical properties, complementary metal oxide semiconductors, differential interference contrast microscopes, localized surface plasmon resonance, plasmonic particles, silane coupling agent, tunable optical properties, chemistry, light related phenomena, molecular probe, tumor cell line, nanotubes, optical phenomena Disciplines Chemistry CommentsReprinted (adapted) with permission from Analytical Chemistry 87 (2015) ABSTRACT: Three-layer core−shell plasmonic nanorods (Au/Ag/SiO 2 −NRs), consisting of a gold nanorod core, a thin silver shell, and a thin silica layer, were synthesized and used as optical imaging probes under a differential interference contrast microscope for single particle orientation and rotational tracking. The localized surface plasmon resonance modes were enhanced upon the addition of the silver shell, and the anisotropic optical properties of gold nanorods were maintained. The silica coating enables surface functionalization with silane coupling agents and provides enhanced stability and biocompatibility. Taking advantage of the longitudinal LSPR enhancement, the orientation and rotational information of the hybrid nanorods on synthetic lipid bilayers and on live cell membranes were obtained with millisecond temporal resolution using a scientific complementary metal-oxide-semiconductor camera. The results demonstrate that the as-synthesized hybrid nanorods are promising imaging probes with improved sensitivity and good biocompatibility for single plasmonic particle tracking experiments in biological systems.S

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Resolving Rotational Motions of Nano-objects in Engineered Environments and Live Cells with Gold Nanorods and Differential Interference Contrast Microscopy

Cited by 159 publications

References 39 publications

A Novel Intracellular Peptide Derived from G1/S Cyclin D2 Induces Cell Death

A Novel Intracellular Peptide Derived from G1/S Cyclin D2 Induces Cell Death

Rotational dynamics of cargos at pauses during axonal transport

Multishell Au/Ag/SiO₂ Nanorods with Tunable Optical Properties as Single Particle Orientation and Rotational Tracking Probes

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Resolving Rotational Motions of Nano-objects in Engineered Environments and Live Cells with Gold Nanorods and Differential Interference Contrast Microscopy

Cited by 159 publications

References 39 publications

A Novel Intracellular Peptide Derived from G1/S Cyclin D2 Induces Cell Death

A Novel Intracellular Peptide Derived from G1/S Cyclin D2 Induces Cell Death

Rotational dynamics of cargos at pauses during axonal transport

Multishell Au/Ag/SiO2 Nanorods with Tunable Optical Properties as Single Particle Orientation and Rotational Tracking Probes

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Multishell Au/Ag/SiO₂ Nanorods with Tunable Optical Properties as Single Particle Orientation and Rotational Tracking Probes