Resolving physical interactions between bacteria and nanotopographies with focused ion beam scanning electron microscopy

Jenkins, Joshua; Ishak, Mohd I.; Eales, Marcus; Gholinia, Ali; Kulkarni, S.; Keller, Thomas F.; May, Paul; Nobbs, Angela H.; Su, Bo

doi:10.1016/j.isci.2021.102818

Cited by 11 publications

(17 citation statements)

References 37 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Techniques of evaluation then progressed into methods that can reveal the nanostructure–bacteria interaction through cross-sectional imaging, e.g., focused ion beam (FIB). Recently, slice-by-slice FIB-SEM data reconstruction was used to better observe the interaction of nanopillars with single cells, leading to an improvement from the previous postulations. Several hypotheses then emerged, one of which is specific to Gram-negative motile bacteria .…”

Section: Mechanisms Revealed From the Experimental Approachesmentioning

confidence: 99%

Critical Review of Nanopillar-Based Mechanobactericidal Systems

Hawi

Goel

Kumar

et al. 2022

ACS Appl. Nano Mater.

View full text Add to dashboard Cite

The rise of multidrug-resistant bacteria is the biggest threat to human health globally, as described by the World Health Organization. Mechanobactericidal surfaces provide a sustainable approach to addressing this concern by eradicating pathogens, especially bacteria, “right-at-the-point” of contacting the surface. However, the lack of a “design to manufacture” approach due to our limited understanding of the mechanobactericidal mechanism has impeded engineering optimization to develop scalable exploitation routes in various healthcare applications. It can be argued that the reason, most particularly, is the limitations and uncertainties associated with the current instrumentation and simulation capabilities, which has led to several streams of test protocols. This review highlights the current understanding on the mechanobactericidal mechanisms in light of the contributing factors and various techniques that are used to postulate these mechanisms. The review offers a critique on the variations observed on how nanostructured surfaces found in the literature have been evaluated such that the test protocols and outcomes are incomparable. The review also shows a strong need for developing more accurate models of a bacterium because the currently reported experimental data are insufficient to develop bacterial material models (constitutive equations). The review also alludes to the scarcity of direct experimental evidence of the mechanobactericidal mechanism, suggesting a strong need for further in situ monitoring as a future research direction.

show abstract

Section: Mechanisms Revealed From the Experimental Approachesmentioning

confidence: 99%

Critical Review of Nanopillar-Based Mechanobactericidal Systems

Hawi

Goel

Kumar

et al. 2022

ACS Appl. Nano Mater.

View full text Add to dashboard Cite

show abstract

“…Smooth surface function was applied to the object to reduce jagged edges of the 3D model. The 3D models were exported in .ply format to be used in other 3D modelling software packages including Blender V2.9 (The Blender Foundation) and Paint 3D (Microsoft Corporation) for recolouring purposes [32].…”

Section: Fib-sem 3d Volume Renderingmentioning

confidence: 99%

Insights into complex nanopillar-bacteria interactions: Roles of nanotopography and bacterial surface proteins

Ishak

Jenkins

Kulkarni

et al. 2021

Journal of Colloid and Interface Science

Self Cite

View full text Add to dashboard Cite

“…[ 19 ] However, we show here that with needles of the correct size (3–4 μm), distribution (0.25–0.5 μm), surface hydrophobicity (≈140°), termination (fluorinated), and diamond coating (4–10 nm), Gram‐positive bacteria are equally, if not more, susceptible to this mechanism of bacterial death, as the bacterial walls of these nonmotile bacteria are more rigid and as a consequence will rupture with less stretching. [ 9 ]…”

Section: Resultsmentioning

confidence: 99%

Antimicrobial Studies of Black Silicon and Black Diamond Using Gram‐Positive Bacteria

Norouzi,

Woudstra,

Smith

et al. 2023

Adv Eng Mater

Self Cite

View full text Add to dashboard Cite

Bactericidal surfaces are in high demand for biomedical as well as industrial applications. In this paper, we have investigated if black diamond is useful for this application. Black diamond is derived from black silicon, a silicon surface structured into nano‐sized needles. These needles can pierce through bacteria on contact. Black diamond is obtained by coating black silicon with a thin diamond film rendering the nanostructures more robust. We compared the bactericidal and anti‐bacterial properties of fluorine‐terminated and hydrogen‐terminated black diamonds with those for black silicon and for flat surfaces of diamond (on silicon) with the same terminations. In this study we evaluated the ability to repel and kill Gram‐positive S. aureus and S epidermidis, which have a thicker cell wall and are more mechanically robust than the bacteria that have been studied before. We evaluated the short‐term 1 h initial adhesion as well as long‐term 24 h biofilm formation. We found that the number of bacteria that initially adhered to the fluorine‐terminated black diamond surface was significantly reduced and had the highest dead bacterial ratio compared to fluorine‐terminated flat diamond, black silicon surfaces and hydrogen‐terminated diamond surfaces, respectively. Biofilm formation after 24 h showed that while all surfaces outperform glass over the long‐term (24 h), diamond‐coated surfaces with both fluorine and hydrogen termination have a significant inhibiting biofilm formation effect. In conclusion, fluorinated and hydrogenated diamond‐coated surfaces with and without nano‐needles have repelling, bactericidal and biofilm‐inhibiting effects on Gram‐positive bacterial strains and are promising antimicrobial surfaces.This article is protected by copyright. All rights reserved.

show abstract

Resolving physical interactions between bacteria and nanotopographies with focused ion beam scanning electron microscopy

Cited by 11 publications

References 37 publications

Critical Review of Nanopillar-Based Mechanobactericidal Systems

Critical Review of Nanopillar-Based Mechanobactericidal Systems

Insights into complex nanopillar-bacteria interactions: Roles of nanotopography and bacterial surface proteins

Antimicrobial Studies of Black Silicon and Black Diamond Using Gram‐Positive Bacteria

Contact Info

Product

Resources

About