Resolution of quantitative resistance to clubroot into QTL-specific metabolic modules

Wagner, Geoffrey; Laperche, Anne; Lariagon, Christine; Marnet, Nathalie; Renault, David; Guitton, Yann; Bouchereau, Alain; Delourme, Régine; Manzanares-Dauleux, Maria; Gravot, Antoine

doi:10.1093/jxb/erz265

Cited by 24 publications

(19 citation statements)

References 94 publications

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“…Yudal (B. napus var. oleifera) is a native rapeseed inbred line of Korean origin (Jeong et al 2012;Wagner et al 2019), developed as a high erucic acid Brassica napus cultivar in 1969 (Choy et al 2007;Kae et al 1971). As a native allotetraploid B. napus variety, Yudal's A-genome presumably derived from the natural hybridisation of B. rapa and B. oleracea, ~ 5000-10,000 years ago.…”

Section: Tuyv Resistance Inheritance and Qtlsmentioning

confidence: 99%

Identification and QTL mapping of resistance to Turnip yellows virus (TuYV) in oilseed rape, Brassica napus

et al. 2019

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Key message Partially dominant resistance to Turnip yellows virus associated with one major QTL was identified in the natural allotetraploid oilseed rape cultivar Yudal. Abstract Turnip yellows virus (TuYV) is transmitted by the peach-potato aphid (Myzus persicae) and causes severe yield losses in commercial oilseed rape crops (Brassica napus). There is currently only one genetic resource for resistance to TuYV available in brassica, which was identified in the re-synthesised B. napus line 'R54'. In our study, 27 mostly homozygous B. napus accessions, either doubled-haploid (DH) or inbred lines, representing a diverse subset of the B. napus genepool, were screened for TuYV resistance/susceptibility. Partial resistance to TuYV was identified in the Korean spring oilseed rape, B. napus variety Yudal, whilst the dwarf French winter oilseed rape line Darmor-bzh was susceptible. QTL mapping using the established Darmor-bzh × Yudal DH mapping population (DYDH) revealed one major QTL explaining 36% and 18% of the phenotypic variation in two independent experiments. A DYDH line was crossed to Yudal, and reciprocal backcross (BC 1) populations from the F 1 with either the susceptible or resistant parent revealed the dominant inheritance of the TuYV resistance. The QTL on ChrA04 was verified in the segregating BC 1 population. A second minor QTL on ChrC05 was identified in one of the two DYDH experiments, and it was not observed in the BC 1 population. The TuYV resistance QTL in 'R54' is within the QTL interval on Chr A04 of Yudal; however, the markers co-segregating with the 'R54' resistance are not conserved in Yudal, suggesting an independent origin of the TuYV resistances. This is the first report of the QTL mapping of TuYV resistance in natural B. napus.

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Section: Tuyv Resistance Inheritance and Qtlsmentioning

confidence: 99%

Identification and QTL mapping of resistance to Turnip yellows virus (TuYV) in oilseed rape, Brassica napus

et al. 2019

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“…The effective and sustainable clubroot management by clubroot resistant cultivars is now disclosed, and hints at the importance of resistant cultivars for clubroot management. On the other hand, the host-range of the pathogen is mostly restricted within Brassicaceae species [ 19 , 23 , 24 ].…”

Section: Infection Process Of the Pathogensmentioning

confidence: 99%

Genetics of Clubroot and Fusarium Wilt Disease Resistance in Brassica Vegetables: The Application of Marker Assisted Breeding for Disease Resistance

Mehraj

Akter

Miyaji

et al. 2020

Plants

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The genus Brassica contains important vegetable crops, which serve as a source of oil seed, condiments, and forages. However, their production is hampered by various diseases such as clubroot and Fusarium wilt, especially in Brassica vegetables. Soil-borne diseases are difficult to manage by traditional methods. Host resistance is an important tool for minimizing disease and many types of resistance (R) genes have been identified. More than 20 major clubroot (CR) disease-related loci have been identified in Brassica vegetables and several CR-resistant genes have been isolated by map-based cloning. Fusarium wilt resistant genes in Brassica vegetables have also been isolated. These isolated R genes encode the toll-interleukin-1 receptor/nucleotide-binding site/leucine-rice-repeat (TIR-NBS-LRR) protein. DNA markers that are linked with disease resistance allele have been successfully applied to improve disease resistance through marker-assisted selection (MAS). In this review, we focused on the recent status of identifying clubroot and Fusarium wilt R genes and the feasibility of using MAS for developing disease resistance cultivars in Brassica vegetables.

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“…An example of systems biology was carried out for a A. thaliana – S. sclerotiorum pathosystem, where a genome-scale metabolic model of S. sclerotiorum based on global gene expression was constructed to assess the metabolic activity in different parts of the hyphal cells, supporting the hypothesis that cooperation in S. sclerotiorum hyphal cells is necessary for virulence and host colonisation [ 218 ]. A combination of metabolomics with quantitative genetics was used to discover the potential role of gluconasturtiin in the B. napus resistance response against Clubroot and the underlying QTL controlling the trait on chromosome C03 and C09 [ 219 ]. Gluconasturtiin is a type of glucosinolate compound associated with the biotic resistance responses of Brassica species [ 220 ].…”

Section: Application Of Metabolomics and Systems Biology In The mentioning

confidence: 99%

Understanding Host–Pathogen Interactions in Brassica napus in the Omics Era

Neik

Amas

Barbetti

et al. 2020

Plants

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Brassica napus (canola/oilseed rape/rapeseed) is an economically important crop, mostly found in temperate and sub-tropical regions, that is cultivated widely for its edible oil. Major diseases of Brassica crops such as Blackleg, Clubroot, Sclerotinia Stem Rot, Downy Mildew, Alternaria Leaf Spot and White Rust have caused significant yield and economic losses in rapeseed-producing countries worldwide, exacerbated by global climate change, and, if not remedied effectively, will threaten global food security. To gain further insights into the host–pathogen interactions in relation to Brassica diseases, it is critical that we review current knowledge in this area and discuss how omics technologies can offer promising results and help to push boundaries in our understanding of the resistance mechanisms. Omics technologies, such as genomics, proteomics, transcriptomics and metabolomics approaches, allow us to understand the host and pathogen, as well as the interaction between the two species at a deeper level. With these integrated data in multi-omics and systems biology, we are able to breed high-quality disease-resistant Brassica crops in a more holistic, targeted and accurate way.

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Resolution of quantitative resistance to clubroot into QTL-specific metabolic modules

Abstract: Genetic metabolomics identifies a series of QTL-specific metabolic modules associated with quantitative resistance to clubroot, and highlights the possible roles of gluconasturtiin, citric acid, and two unknown compounds in partial resistance.

Cited by 24 publications

References 94 publications

Identification and QTL mapping of resistance to Turnip yellows virus (TuYV) in oilseed rape, Brassica napus

Identification and QTL mapping of resistance to Turnip yellows virus (TuYV) in oilseed rape, Brassica napus

Genetics of Clubroot and Fusarium Wilt Disease Resistance in Brassica Vegetables: The Application of Marker Assisted Breeding for Disease Resistance

Understanding Host–Pathogen Interactions in Brassica napus in the Omics Era

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