Resolution of (±)‐ibuprofen using (−)‐brucine as a chiral selector by thin layer chromatography

Bhushan, Ravi; Gupta, Divender

doi:10.1002/bmc.398

Cited by 20 publications

(14 citation statements)

References 16 publications

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“…For instance, enantioselectivity using brucine arises due to the formation of two diastereomers through simple ionic interactions between racemate and chiral selector, e.g. (+)-citalopram/brucine and (−)-citalopram/brucine 30. The enantiomeric resolution by HP-β-CD may involve the inclusion of drug within the CD cavity relative to the comparability of sizes, shapes and hydrophibicities.…”

Section: Resultsmentioning

confidence: 99%

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Micelle Enhanced Fluorimetric and Thin Layer Chromatography Densitometric Methods for the Determination of (±) Citalopram and its S - Enantiomer Escitalopram

2009

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Two sensitive and validated methods were developed for determination of a racemic mixture citalopram and its enantiomer S-(+) escitalopram. The first method was based on direct measurement of the intrinsic fluorescence of escitalopram using sodium dodecyl sulfate as micelle enhancer. This was further applied to determine escitalopram in spiked human plasma, as well as in the presence of common and co-administerated drugs. The second method was TLC densitometric based on various chiral selectors was investigated. The optimum TLC conditions were found to be sensitive and selective for identification and quantitative determination of enantiomeric purity of escitalopram in drug substance and drug products. The method can be useful to investigate adulteration of pure isomer with the cheap racemic form.

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Section: Resultsmentioning

confidence: 99%

“…The method was validated according to ICH regulations by documenting its linearity, accuracy, precision, limit of detection and quantification, specificity and, robustness 30,33. The good linearity was obtained for seven concentrations in the range of 0.5– 40 μg/spot as shown in Figure 5.…”

Section: Resultsmentioning

confidence: 99%

Micelle Enhanced Fluorimetric and Thin Layer Chromatography Densitometric Methods for the Determination of (±) Citalopram and its S - Enantiomer Escitalopram

2009

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“…In addition, resolution of (±)-ibuprofen using (-)-brucine (Bhushan and Gupta, 2004) and L-arginine (Bhushan and Parshad, 1996) impregnated TLC plates, (±)-verapamil using macrocyclic antibiotic as chiral selector in TLC (Bhushan and Gupta, 2005) and (±)-thioridazine using β-CD based CSP by HPLC (Bhushan and Gupta, 2006b) has also been reported.…”

Section: Introductionmentioning

confidence: 95%

Indirect resolution of baclofen enantiomers from pharmaceutical dosage form by reversed‐phase liquid chromatography after derivatization with Marfey's reagent and its structural variants

Bhushan

Kumar

2008

Biomedical Chromatography

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A high-performance liquid chromatographic (HPLC) method was developed for chiral assay of baclofen enantiomers in pharmaceutical formulations using an indirect approach. Baclofen enantiomers were derivatized with Marfey's reagent (FDNP-L-Ala-NH2) and its structural variants FDNP-L-Phe-NH2, FDNP-L-Val-NH2, FDNP-L-Leu-NH2 and FDNP-L-Pro-NH2. The resultant diastereomers were separated on RP-TLC [triethylammonium phosphate buffer (pH 4.0, 50 mm)-acetonitrile, 50:50] and on a C18 column using a linear gradient (45 min) of acetonitrile and 0.01% aqueous trifluoroacetic acid (TFA) with UV detection at 340 nm. The differences in the retention times (Delta t R) of diastereomers due to the five chiral reagents were compared. The maximum and minimum difference in retention times between separated diastereomers was for FDNP-L-Leu-NH2 and FDNP-L-Pro-NH2, respectively. The effect of flow rate, acetonitrile content and TFA concentration on resolution was studied. The method was validated for linearity, repeatability, limit of detection and limit of quantification.

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“…Enantiomeric resolution of ibuprofen in pharmaceutical preparations has been reported (Bhushan and Gupta, 2004;Bhushan and Thiongo, 1999;Bhushan and Parshad, 1996).…”

Section: Introductionmentioning

confidence: 98%

Liquid chromatographic analysis of certain commercial formulations for non‐opioid analgesics

Bhushan

Gupta

Mukherjee

2007

Biomedical Chromatography

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A simple and sensitive method for separation and quantitative determination of non-opioid analgesics from pharmaceutical preparations has been developed and validated. Commercial formulations of three non-opioid analgesics, viz. paracetamol, ibuprofen and diclofenac, were chosen for present studies. These were extracted, isolated, purified and recrystallized and were characterized by melting point, lambda(max) and IR. Quantitative determination was carried out using HPLC and TLC supplemented with UV spectrophotometry.

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Resolution of (±)‐ibuprofen using (−)‐brucine as a chiral selector by thin layer chromatography

Cited by 20 publications

References 16 publications

Micelle Enhanced Fluorimetric and Thin Layer Chromatography Densitometric Methods for the Determination of (±) Citalopram and its S - Enantiomer Escitalopram

Micelle Enhanced Fluorimetric and Thin Layer Chromatography Densitometric Methods for the Determination of (±) Citalopram and its S - Enantiomer Escitalopram

Indirect resolution of baclofen enantiomers from pharmaceutical dosage form by reversed‐phase liquid chromatography after derivatization with Marfey's reagent and its structural variants

Liquid chromatographic analysis of certain commercial formulations for non‐opioid analgesics

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