Resolution limits for infrared microspectroscopy explored with synchrotron radiation

Carr, G. L.

doi:10.1063/1.1347965

Cited by 217 publications

(186 citation statements)

References 8 publications

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“…The lateral resolution predicted by the diffraction limit would be ∼10 µm at a wavelength of 10 µm if the measurement was carried out confocally, but with an aperture of 10 µm, the light throughput would be compromised for the PE300 instrument. However, confocal IR-MSP measurements have been reported using synchrotron-based IR-MSP (Carr, 2001). …”

Section: F Diffraction Limit and Spatial Resolutionmentioning

confidence: 99%

Chapter 10 Infrared and Raman Microscopy in Cell Biology

Matthäus

Bird

Miljković

et al. 2008

Methods in Cell Biology

163

119

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This chapter presents novel microscopic methods to monitor cell biological processes of live or fixed cells without the use of any dye, stains, or other contrast agent. These methods are based on spectral techniques that detect inherent spectroscopic properties of biochemical constituents of cells, or parts thereof. Two different modalities have been developed for this task. One of them is infrared micro-spectroscopy, in which an average snapshot of a cell's biochemical composition is collected at a spatial resolution of typically 25 mm. This technique, which is extremely sensitive and can collect such a snapshot in fractions of a second, is particularly suited for studying gross biochemical changes. The other technique, Raman microscopy (also known as Raman microspectroscopy), is ideally suited to study variations of cellular composition on the scale of subcellular organelles, since its spatial resolution is as good as that of fluorescence microscopy. Both techniques exhibit the fingerprint sensitivity of vibrational spectroscopy toward biochemical composition, and can be used to follow a variety of cellular processes.

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Section: F Diffraction Limit and Spatial Resolutionmentioning

confidence: 99%

Chapter 10 Infrared and Raman Microscopy in Cell Biology

Matthäus

Bird

Miljković

et al. 2008

Methods in Cell Biology

163

119

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“…Infrared reflectance micro-spectroscopy between 150 and 6000 cm -1 was performed at beamline U12IR at the National Synchrotron Light Source, Brookhaven National Laboratory [39]. Infrared polarizers were employed to obtain reflectance spectra parallel and perpendicular to the long axis of the crystals, i.e.…”

Section: Experimental Methodsmentioning

confidence: 99%

Insulating phases of vanadium dioxide are Mott-Hubbard insulators

et al. 2017

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We present the first comprehensive broadband optical spectroscopy data on two insulating phases of vanadium dioxide (VO 2 ): monoclinic M 2 and triclinic. The main result of our work is that the energy gap and the electronic structure are essentially unaltered by the first-order structural phase transition between the M 2 and triclinic phases. Moreover, the optical interband features in the M 2 and triclinic phases are remarkably similar to those observed in the well-studied monoclinic M 1 insulating phase of VO 2 . As the energy gap is insensitive to the different lattice structures of the three insulating phases, we rule out vanadium-vanadium pairing (the Peierls component) as the dominant contributor to the opening of the gap. Rather, the energy gap arises primarily from intra-atomic Coulomb correlations.

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“…[17][18][19][20][21][22][23][24] However, spatial resolution in transmission mode is lower than that of micro-ATR mode even when measured using a powerful synchrotron source. [25][26] Although a recent report 27 has shown that, by using a multi-beam synchrotron source and a high magnifying power objective, the spatial resolution in transmission mode can approach that of micro-ATR mode.…”

Section: Introductionmentioning

confidence: 99%

Correcting the Effect of Refraction and Dispersion of Light in FT-IR Spectroscopic Imaging in Transmission through Thick Infrared Windows

Chan

Kazarian

2012

Anal. Chem.

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Transmission mode is one of the most common sampling methods for FTIR spectroscopic imaging because the spectra obtained generally have a reasonable signal to noise ratio. However, dispersion and refraction of infrared light occurs when samples are sandwiched between infrared windows or placed underneath a layer of liquid. Dispersion and refraction cause infrared light to focus with different focal lengths depending on the wavelength (wavenumber) of the light. As a result, images obtained are in focus only at a particular wavenumber while they are defocused at other wavenumber values. In this work, a solution to correct this spread of focus by means of adding a lens on top of the infrared transparent window, such that a pseudo hemisphere is formed, has been investigated. Through this lens (or hemisphere), refraction of light is removed and the light across the spectral range has the same focal depth. Furthermore, the lens acts as a solid immersion objective and an increase of both magnification and spatial resolution (by 1.4 times) is demonstrated. The spatial resolution was investigated using an USAF resolution target, showing that the Rayleigh criterion can be achieved, as well as a sample with a sharp polymer interface to indicate the spatial resolution that can be expected in real samples. The reported approach was used to obtain chemical images of cross-sections of cancer tissue and hair samples sandwiched between infrared windows showing the versatility and applicability of the method. In addition to the improved spatial resolution, the results reported herein also demonstrate that the lens can reduce the effect of scattering near the edges of tissue samples. The advantages of the presented approach, obtaining FTIR spectroscopic images in transmission mode with the same focus across all wavenumber values and simultaneous improvement in spatial resolution, will have wide implications ranging from studies of live cells to sorption of drugs into tissues.

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Resolution limits for infrared microspectroscopy explored with synchrotron radiation

Cited by 217 publications

References 8 publications

Chapter 10 Infrared and Raman Microscopy in Cell Biology

Chapter 10 Infrared and Raman Microscopy in Cell Biology

Insulating phases of vanadium dioxide are Mott-Hubbard insulators

Correcting the Effect of Refraction and Dispersion of Light in FT-IR Spectroscopic Imaging in Transmission through Thick Infrared Windows

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