Resolution improvement by using harmonic detection in an ion cyclotron resonance mass spectrometer

“…With innovations in ICR cells, − higher resolution can be obtained with a given magnetic field. In addition, using the frequency-multiple detection technique can improve the resolution of FT-ICR data with the same acquisition time. − In a previous study, FT-ICR MS combined with quadrupolar detection (QPD) was shown to be effective in analyzing complex organic mixtures . The important advantage of using QPD for LC-MS is that data with the same resolution can be obtained with half the acquisition time, which can reduce the acquisition time per scan for LC-MS applications.…”

mentioning

confidence: 99%

Application of Online Liquid Chromatography 7 T FT-ICR Mass Spectrometer Equipped with Quadrupolar Detection for Analysis of Natural Organic Matter

et al. 2019

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In this study, Fourier transform ion cyclotron resonance mass spectrometry (FT-ICR MS), combined with quadrupolar detection (QPD), was applied for online liquid chromatography (LC) MS analysis of natural organic matter (NOM). Although FT-ICR MS has emerged as an important analytical technique to study NOM, there are few previous reports on online LC FT-ICR MS analysis of NOM due to the long acquisition time (2–8 s) required to obtain high-resolution mass spectra. The QPD technique provides a critical advantage over the conventional dipolar detection (DPD) technique for LC-MS analysis because a spectrum with the same resolving power can be obtained in approximately half the acquisition time. QPD FT-ICR MS provides resolving powers ( ) of ∼300000 and 170000 at m/z 400 with acquisition times per scan of 1.2 and 0.8 s, respectively. The reduced acquisition time per scan allows increased number of acquisitions in a given LC analysis time, resulting in improved signal to noise (S/N) ratio and dynamic range in comparison to conventional methods. For example, 40% and 100% increases in the number of detected peaks were obtained with LC QPD FT-ICR MS, in comparison to conventional LC DPD FT-ICR MS and direct-injection FT-ICR MS. It is also possible to perform more quantitative comparison and molecular level investigation of NOMs with 2 μg of a NOM sample. The data presented herein demonstrate a proof of principle that QPD combined with LC FT-ICR MS is a sensitive analytical technique that can provide comprehensive information about NOM.

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“…Alternative arrangements for the detection of integer multiples or harmonics of the fundamental cyclotron frequency have been developed by Chen et al (160). Multiple detection electrode designs have been promoted by independent groups to increase detection signal at selected harmonics (148,161) as well as to improve spectral resolution (66,70,128,162). Some cell designs have included up to 18 electrodes (70).…”

Section: A Alternative Closed Cell Geometriesmentioning

confidence: 99%