Resistin mediates tomato and broccoli extract effects on glucose homeostasis in high fat diet-induced obesity in rats

Abstract

BackgroundResistin is an adipocyte hormone that regulates glucose metabolism. Elevated levels of resistin may cause insulin resistance. This may link obesity, and increased fat mass to type II diabetes and insulin resistance. We hypothesized that treatment with tomato and broccoli extracts regulates glucose homeostasis via modulation of resistin levels in high fat diet-induced obesity rats (HFD).MethodsForty-eight male albino rats were divided into 8 groups as follows: control, HFD, stop fat diet (SD), Tomato 200 mg/kg (T200), Tomato 400 mg/kg (T400), Broccoli 200 mg/kg (B200), Broccoli 400 mg/kg (B400), and Chromax (CX). Treatment continued for 1 month. Serum levels of resistin, leptin, adiponectin, glucose and insulin were measured using ELISA and spectrophotometry.ResultsSerum levels of resistin were significantly reduced in the T 200, T 400, B 200, B 400 and CX groups to: 4.13 ± 0.22 ng/ml, 1.51 ± 0.04 ng/ml, 4.13 ± 0.22 ng/ml, 2.32 ± 0.15 ng/ml and 1.37 ± 0.03 ng/ml, respectively, compared to HFD group and SD group (P value < 0.0001). Non-significant differences were found between T 400, B 400 and CX groups. Serum levels of leptin were significantly reduced in the T 400 (22.7 ± 0.84 pg/ml) group compared to the B 400 (41 ± 2.45 Pg/ml) and CX groups (45.7 ± 2.91 Pg/ml), P value < 0.001. Serum levels of adiponectin were significantly increased in the T 400 group (131 ± 3.84 pg/ml) compared to the CX group (112 ± 4.77 pg/ml), P value < 0.01.ConclusionsOur results demonstrate that tomato and broccoli extract treatment regulates glucose homeostasis via reduction of serum resistin and may be a useful non-pharmacological therapy for obesity.Electronic supplementary materialThe online version of this article (doi:10.1186/s12906-016-1203-0) contains supplementary material, which is available to authorized users.

Anti-obesity potential of Moringa olifera seed extract and lycopene on high fat diet induced obesity in male Sprauge Dawely rats

“…Increasing fat mass in HFD fed rats led to increased serum leptin and resistin levels, and this coincided with the results of Aborehab et al, 2016 , Santos et al, 2019 . Increasing fat mass in the current study led to promotion of inflammatory cytokines as well as adipokines production such as leptin ( Saucillo et al, 2014 ) and resistin ( Ribot et al, 2008 ).…”

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“…Changes in adipocyte activity and hypertrophy may alter the function of adipokines secreted by this cell leading to changes in basal metabolism and insulin signaling, triggering an increase in appetite and consequent gain in body weight (Peyot et al ., ). Evidences have shown that HFD‐fed mice have high levels of leptin (Oliveira et al ., ; Alwahsh et al ., ) and resistin (Aborehab et al ., ) and decreased levels of adiponectin, which favor the deregulation in glucose and insulin metabolism (Emiliano et al ., , De Las Heras et al ., ). Consistent with these previous studies, we found that plasma levels and adipose tissue expression of adiponectin were decreased in HFD fed mice, while plasma levels and adipose tissue expression of leptin were increased in the same group.…”

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