A putative Chondroprotective role for IL-1β and MPO in herbal treatment of experimental osteoarthritis

Abstract

BackgroundHerbal treatment may have a chondroprotective and therapeutic effect on Osteoarthritis (OA). We investigated the mechanism of action of ginger and curcumin rhizomes cultivated in Egypt in treatment of OA in rat model.MethodsThirty-five albino rats were intra-articularly injected with Monosodium Iodoacetate in the knee joint. Ginger and curcumin was orally administered at doses of 200 and 400 mg/kg (F200 and F400). Serum levels of cartilage oligomeric matrix protein (COMP), hyaluronic acid (HA), malondialdehyde (MDA), myeloperoxidase (MPO), Interleukin-1 beta (IL-1β) and superoxide dismutase activity (SOD) were measured using ELISA. The composition of the herbal formula hydro-ethanolic extract was characterized using UPLC-ESI-MS. Histopathological changes in injected joints was examined using routine histopathology. Statistical analysis was performed using one-way ANOVA.ResultsSerum levels of COMP, HA, MPO, MDA, and IL-1β were significantly decreased in F 200, F 400 and V groups when compared to OA group (P value <0.0001). On the other hand SOD levels were significantly elevated in treated groups compared to OA groups (P value <0.0001).ConclusionsThe ginger/curcumin at 1:1 had chondroprotective effect via anti-inflammatory and antioxidant effect in rat OA model. Further pharmacological and clinical studies are needed to evaluate this effect.

Selection and Investigation of a Primate Model of Spontaneous Degenerative Knee Osteoarthritis, the Cynomolgus Monkey (Macaca Fascicularis)

“…Worldwide, osteoarthritis (OA), including degenerative knee osteoarthritis (KOA), is a chronic progressive joint disease, which involves inflammation, pain, and degenerative structural changes in the joint, resulting in functional disability [ 1 , 2 ]. Osteoarthritis commonly affects the weight-bearing joints, particularly the knee [ 3 , 4 ]. In the US, by the year 2040, the prevalence of diagnosis of osteoarthritis is expected to rise to 78.4 million, or 25.9% of all adults [ 5 ].…”

The extract from the roots of Rose odorata sweet var. gigantean (Coll. et Hemsl.) Rehd. et Wils attenuates DSS-induced ulcerative colitis by regulating the Nrf2/NF-κB signaling pathways

“…31 NO could lead to tissue damage in the middle and distal colon through the inltration of neutrophils, and MPO is a well-established biomarker of neutrophil inltration for evaluating inammation in humans and animal models of UC. 32 In this study, our data showed that the activity of SOD in the model group was much lower, meanwhile the contents of MDA, MPO, and NOS were signicantly higher than those of the control group, indicating the oxidative stress in the UC mouse model. Aer treatment with ROE, the SOD activity was increased, and the levels of MDA, MPO, and NOS were decreased.…”

Hyaluronic acid-chitosan nanoparticles encoding CrmA attenuate interleukin-1β induced inflammation in synoviocytes in�vitro

“…IL-1β serves a key role in the pathogenesis and progression of OA (40). It may stimulate chondrocytes and synoviocytes to secrete proteinases leading to cartilage destruction (41), and inhibit the synthesis of collagen type II (42), which is the primary component of the ECM in articular cartilage.…”