Resistencia a compuestos azólicos de aislamientos clínicos de Trichophyton spp

Méndez-Tovar, Luis Javier; Manzano-Gayosso, Patricia; Velásquez-Hernández, Verónica; Millán-Chiu, Blanca E.; Hernández-Hernández, Francisca; Mondragón-González, Rafael; López-Martínez, Rubén

doi:10.1016/s1130-1406(07)70065-7

Cited by 17 publications

(9 citation statements)

References 9 publications

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“…A pesar de que la comunicación de casos con mala respuesta terapéutica va en aumento a nivel mundial, 6,7 hasta el momento son pocos los estudios de sensibilidad realizados para demostrar su resistencia; entre estos, cabe mencionar dos que reportan resistencia a azoles en aislamientos de T. rubrum. 8,9 La escasa comunicación obedece en parte a la dificultad que ofrece el montaje de las pruebas de sensibilidad de los hongos filamentosos, donde hay que considerar variables como tiempo y temperatura de incubación de la prueba, medio adecuado para la producción de aleurias y macroaleuriosporas de los dermatofitos, entre otras. 10,11 Actualmente el método de referencia para los hongos filamentosos es el documento M38-A2 del Instituto de Estándares de Laboratorios Clínicos (Clinical Laboratory Standards Institute, CLSI, por sus siglas en inglés).…”

Section: Fecha Recibido: 26 De Febrero De 2013unclassified

“…12 Sin embargo, su utilidad para los dermatofitos se limita únicamente a fines investigativos y no constituye aún una prueba de rutina. Dada la frecuencia del uso de fluconazol en la población costarricense y la baja sensibilidad de T. rubrum al fluconazol, reportada por otros autores, 8,9 en el presente estudio se investigó el perfil de resistencia al fluconazol de cepas T. rubrum aisladas en el medio nacional.…”

Section: Fecha Recibido: 26 De Febrero De 2013unclassified

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Sensibilidad al fluconazol de aislamientos de Trichophyton rubrum

Gross-Martínez

Ureña-Sánchez

Chaves-Madrigal

2020

AMC

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Section: Fecha Recibido: 26 De Febrero De 2013unclassified

Sensibilidad al fluconazol de aislamientos de Trichophyton rubrum

Gross-Martínez

Ureña-Sánchez

Chaves-Madrigal

2020

AMC

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“…In addition, the occurrence of resistance or side effects in clinically isolated strains leads to failure in the treatment of mycoses. [3][4][5][6][7] Apart from side-effects like liver-damage or affecting estrogen levels, many medicines can cause allergic reactions. For example, the azole group of drugs is known to cause anaphylaxis.…”

Section: Introductionmentioning

confidence: 99%

Antifungal activity of Lactobacillus againstMicrosporum canis,Microsporum gypseumandEpidermophyton floccosum

et al. 2012

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A total of 220 lactic acid bacteria isolates were screened for antifungal activity using Aspergillus fumigatus and Aspergillus niger as the target strains. Four Lactobacillus strains exhibited strong inhibitory activity on agar surfaces. All four were also identified as having strong inhibitory activity against the human pathogenic fungi Microsporum canis, Microsporum gypseum and Epidermophyton floccosum. One of the four lactobacilli, namely Lb. reuteri ee1p exhibited the most inhibition against dermatophytes. Cell-free culture supernatants of Lb. reuteri ee1p and of the non-antifungal Lb. reuteri M13 were freeze-dried and used to access and compare antifungal activity in agar plate assays and microtiter plate assays. Addition of the Lb. reuteri ee1p freeze-dried cell-free supernatant powder into the agar medium at concentrations greater than 2% inhibited all fungal colony growth. Addition of the powder at 5% to liquid cultures caused complete inhibition of fungal growth on the basis of turbidity. Freeze-dried supernatant of the non-antifungal Lb. reuteri M13 at the same concentrations had a much lesser effect. As Lb. reuteri M13 is very similar to the antifungal strain ee1p in terms of growth rate and final pH in liquid culture, and as it has little antifungal activity, it is clear that other antifungal compounds must be specifically produced (or produced at higher levels) by the anti-dermatophyte strain Lb. reuteri ee1p. Reuterin was undetectable in all four antifungal strains. The cell free supernatant of Lb. reuteri ee1p was analyzed by LC-FTMS using an Accela LC coupled to an LTQ Orbitrap XL mass spectrometer. The high mass accuracy spectrum produced by compounds in the Lb. reuteri ee1p strain was compared with both a multianalyte chromatogram and individual spectra of standard anti-fungal compounds, which are known to be produced by lactic acid bacteria. Ten antifungal metabolites were detected.

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“…Itraconazole capsules, for instance, are given in a pulse-treatment regimen (400 mg/day for 1 week at monthly intervals) that is repeated twice or three times for finger nail onychomycosis and is repeated three or four times for toenail onychomycosis [4–6]. Because some strains of Trichophyton spp., however, are resistant to azole compounds [7] and itraconazole has toxic side effects [8], we want to provide a new tool for the treatment of onychomycosis. Antifungal effects of reactive oxygen species have long been documented [9–13], and hydroxyl radicals are known to damage proteins, lipids, and nucleic acids [14–17].…”

Section: Introductionmentioning

confidence: 99%

Fungicidal Action of Hydroxyl Radicals Generated by Ultrasound in Water

Iwasawa

Saito

Mokudai

et al. 2009

J. Clin. Biochem. Nutr.

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It is well known that hydroxyl radicals are generated by ultrasound in water. This study with an electron spin resonance spin-trapping technique showed that hydroxyl radical generation was positively correlated with ultrasound duration and water temperature. The clear fungicidal action against Trichophyton spp. evident by studying cultured cells and the degradation of cytoplasmic and surface structures observed by transmission and scanning electron microscopy suggest that ultrasound in hot water is effective for sterilization of dermatophyte contamination and could be effective for the treatment of tinea infection.

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Resistencia a compuestos azólicos de aislamientos clínicos de Trichophyton spp

Cited by 17 publications

References 9 publications

Sensibilidad al fluconazol de aislamientos de Trichophyton rubrum

Sensibilidad al fluconazol de aislamientos de Trichophyton rubrum

Antifungal activity of Lactobacillus againstMicrosporum canis,Microsporum gypseumandEpidermophyton floccosum

Fungicidal Action of Hydroxyl Radicals Generated by Ultrasound in Water

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