Fungicidal Action of Hydroxyl Radicals Generated by Ultrasound in Water

Iwasawa, Atsuo; Saito, Keita; Mokudai, Takayuki; Kohno, Masahiro; Ozawa, Toshihiko; Niwano, Yoshimi

doi:10.3164/jcbn.08-261

Cited by 23 publications

(17 citation statements)

References 20 publications

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“…A PAC-associated detrimental effect on the structural integrity of the capsid was proposed. The antiviral activity of comestible juices, juice drinks, and their PACs appears to be nonspecific, in that they cause different reductions in infectivity titers of numerous bacterial and animal viruses (De Bryne et al 1999;Iwasawa et al 2009;Speirs 1976, 1978;Lipson et al 2007bLipson et al , 2010Su et al 2010a). Differences in juice-or PAC-mediated antiviral activity may be reflective of the viral species, the host cell type in relation to efficiency of plating (see Danis et al 1997), the plant species utilized to obtain the polyphenolics [e.g., V. macrocarpon (cranberry), V. labrusca (grape)], and/or the method of extraction/ purification of the polyphenolic.…”

Section: Resultsmentioning

confidence: 99%

Cranberry and Grape Juices Affect Tight Junction Function and Structural Integrity of Rotavirus-Infected Monkey Kidney Epithelial Cell Monolayers

et al. 2011

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Cranberry juice (CJ) and grape juice (GJ) from Vaccinium macrocarpon and Vitis labrusca, respectively, and purified proanthocyanidins (PACs) from these species are recognized to possess antiviral activity. The effects of CJ and GJ on tight junction (TJ) structure and function among rotavirus-infected monkey kidney epithelial cells in monolayer cultures were evaluated. Antiviral activity by cranberry PACs of rotavirus in cell-free suspension was investigated by a rotavirus antigen [i.e., viral capsid protein 6 (VP6)] capture enzyme-linked immunosorbent assay (ELISA) and by transmission electron microscopy (TEM). MA-104 monolayers were treated with CJ, GJ, or cranberry juice cocktail (CJC) drink before inoculation with rotavirus. TJ function and structural integrity were measured by changes in transepithelial electrical resistance (TEER) and by reduction of signal intensity of the TJ a-claudin 1 by immunofluorescence. The inhibitory activity of CJ and GJ on viral RNA synthesis, as a function of viral concentration, was determined by reverse transcription polymerase chain reaction (rtPCR). After 4 days, virus-infected monolayers pretreated with GJ (Concord and Niagara GJs) had TEER readings similar to uninfected controls. CJ and CJC also had a significant protective effect (P \ 0.05) on TJ function, but to a lesser extent than GJ. Disorganization of TJ integrity commenced at 24-to 36-h post-viral inoculation, but this effect was reduced by pretreatment with CJ or GP of monolayer cultures. TEM showed aggregation of rotavirus by cranberry PACs. The destruction of rotavirus capsid proteins VP6, in cell-free suspension was inversely related to the concentration of cranberry PACs (C-PAC). Loss of rotavirus RNA by CJ or GJ was inversely related to viral infectivity titers. CJ, GJ, or PAC-associated antiviral activity has been linked to modifications in cellular physiologic events and to physical factors (e.g., PAC-mediated viral aggregation) that probably compromise viral infectivity. Multiple cell physiological and physical events must be considered when determining the mechanisms associated with the antiviral (i.e., rotavirus) activity of CJ, GJ, and PACs.

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Section: Resultsmentioning

confidence: 99%

Cranberry and Grape Juices Affect Tight Junction Function and Structural Integrity of Rotavirus-Infected Monkey Kidney Epithelial Cell Monolayers

et al. 2011

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“…In this context, we examined fungicidal effect of ultrasound irradiation at a frequency of 1.6 MHz [62] to develop a treatment of onychomycosis (i.e. fungal nail infections).…”

Section: Sonolysis Of Watermentioning

confidence: 99%

Microbicidal Activity of Artificially Generated Hydroxyl Radicals

Sheng

Nakamura

Kanno

et al. 2015

Interface Oral Health Science 2014

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The hydroxyl radical, one of the reactive oxygen species, has one unpaired electron in the structure, so that it tends to deprive other substances of an electron which is so-called oxidation. It is known that hydroxyl radicals produced by immunological response kill invading microorganisms by the oxidation. Besides the immune system, it has been demonstrated that hydroxyl radicals play an important role in the bactericidal action of antibiotics. In this context, we have conducted a research to develop disinfection techniques utilizing artificially generated hydroxyl radicals. We adopted photolysis of H 2 O 2 , sonolysis of water and the other photo-chemical reaction as generators of hydroxyl radicals. A series of studies demonstrated that the microbicidal activity of hydroxyl radicals was sufficient to kill bacteria in an experimental biofilm as well as planktonic bacteria and fungi within a short-treatment time. In addition, the safety aspect is confirmed by an in vivo study and a literature review. Thus, it is suggested that disinfection treatment utilizing artificially generated hydroxyl radicals can be applicable to medical/dental therapy as novel disinfection treatments.

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“…Applying the oxidative power of hydroxyl radicals to disinfection systems has been attempted by utilizing sonolysis of water and photolysis of hydrogen peroxide (H 2 O 2 ) in our laboratory (Kohno et al, 2011;Shirato et al, 2012;Ikai et al, 2010;Iwasawa et al, 2009). In the latter system, designed for the treatment of periodontitis, hydroxyl radicals generated by the photolysis of 1 M H 2 O 2 increased with laser irradiation time and could kill oral pathogenic bacteria in as little as 1 to 3 min.…”

Section: Introductionmentioning

confidence: 99%