Resistansi Escherichia coli asal feses sapi di wilayah Bogor terhadap antimikrob

Abstract

Resistansi bakteri terhadap antimikrob telah menjadi permasalahan global. Pengujian resistansi antimikrob bakteri dari hewan penting dilakukan terutama dengan adanya resistansi antimikrob pada manusia yang diduga bersumber pada ternak. Tujuan penelitian ini adalah untuk menganalisis gambaran resistansi Escherichia coli (E. coli) yang diisolasi dari feses sapi terhadap berbagai antimikrob, yaitu aztreonam, basitrasin, sefpodoksim, enrofloksasin, fosfomisin, gentamisin, dan kloramfenikol. Uji resistansi antimikrob dilakukan menggunakan metode difusi cakram Kirby-Bauer. Hasil uji diinterpretasi menurut Clinical and Laboratory Standards Institute (CLSI). Isolat E. coli asal feses sapi yang diperiksa resistan terhadap aztreonam (40%), sefpodoksim (40%), fosfomisin (50%), basitrasin (100%), dan gentamisin (10%). Isolat E. coli sebanyak 30% memiliki kepekaan intermediat terhadap fosfomisin. Seluruh isolat sensitif terhadap kloramfenikol (100%), dan sebagian besar sensitif terhadap enrofloksasin (90%) dan gentamisin (90%). Isolat E. coli yang resistan terhadap aztreonam dan sefpodoksim diduga berfenotip extended spectrum β-lactamase (ESBL). Pengobatan infeksi E. coli pada sapi harus memperhatikan gambaran resistansi isolat terhadap antimikrob.

Resistance profile of Escherichia coli isolated from stool, feed, and compost sources to antibiotics in Sukabumi

“…The findings revealed that all the samples exhibited susceptibility to chloramphenicol and an intermediate susceptibility to clindamycin. Previous research conducted by Rotinsulu et al (2022) [23] has also shown considerable sensitivity to chloramphenicol in Indonesia. Moreover, a study by Akinyemi (2020) [35] found that the E. coli strain CM2555, which carried the acetyltransferase (cat) gene, was sensitive to chloramphenicol.…”

“…Bacitracin, which is known for its bactericidal properties, disrupts the function of cell membranes, hinders protein synthesis, and restricts the growth of cell walls by blocking the synthesis of peptidoglycan strands [22]. Bacitracin is ineffective against gram-negative bacteria including E. coli [23]. However, its presence in livestock growth promoters or therapy combined with neomycin and polymyxin B needs special attention [22].…”

Multidrug-Resistant Staphylococcus Aureus Isolated From Cattle Milk in Indonesia

“…Inappropriate use of antimicrobials causes antimicrobial resistance, which is now a global problem. Previous studies reported that antimicrobial-resistant bacteria were isolated from cattle in Indonesia (Klobongona et al, 2019;Tyasningsih et al, 2021;Rotinsulu et al, 2022). In order to maximise the benefits of antimicrobial stewardship, continuous monitoring of bacterial pathogens for antimicrobial resistance is essential.…”