Resistance to pyrethroid and organophosphate insecticides, and the geographical distribution and polymorphisms of target-site mutations in voltage-gated sodium channel and acetylcholinesterase 1 genes in Anopheles sinensis populations in Shanghai, China

Fang, Yuan; Shi, Wei; Wu, Jia-Tong; Li, Yuanyuan; Xue, Jing-Bo; Zhang, Yi

doi:10.1186/s13071-019-3657-7

Cited by 25 publications

(20 citation statements)

References 32 publications

(61 reference statements)

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“…192 mosquitoes were genotyped by amplicon sequencing for known mutations associated with resistance to different classes of insecticides: pyrethroids, carbamates, organochlorides, and organophosphates. We genotyped previously characterized mutations in ace-1, kdr, and rdl genes, G119S 45 , L1014F 45 and A296S 52 , respectively. Of the 192 individuals, 187 were successfully genotyped for G119S, 189 for L1014F, and 191 for A296S.…”

Section: Resultsmentioning

confidence: 99%

“…Of the 844 samples that were molecularly typed for species, a randomly chosen subset of 192 samples representative of species and collection location were sequenced for the presence of known insecticide resistance mutations for ace-1 G119S, rdl A296S, and kdr L1014F (see supplementary information for PCR and sequencing details). These mutations were chosen as they are well described and have been associated with insecticide resistance 45 – 47 . All sequence reads were obtained using standard Sanger sequencing methods and resulting sequence traces were visually verified prior to examination of known insecticide resistance mutations.…”

Section: Methodsmentioning

confidence: 99%

“…All heterozygote calls were verified with a second PCR amplification and sequencing. The G119S mutation within the acetylcholinesterase subunit 1 ( ace-1 ) gene has been previously shown to be associated with insecticide resistance 45 . The A296S mutation in exon seven of the rdl gene has previously been shown to be associated with dieldrin resistance.…”

Section: Methodsmentioning

confidence: 99%

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Anopheles ecology, genetics and malaria transmission in northern Cambodia

Vantaux

Riehle

Piv

et al. 2021

Sci Rep

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In the Greater Mekong Subregion, malaria cases have significantly decreased but little is known about the vectors or mechanisms responsible for residual malaria transmission. We analysed a total of 3920 Anopheles mosquitoes collected during the rainy and dry seasons from four ecological settings in Cambodia (villages, forested areas near villages, rubber tree plantations and forest sites). Using odor-baited traps, 81% of the total samples across all sites were collected in cow baited traps, although 67% of the samples attracted by human baited traps were collected in forest sites. Overall, 20% of collected Anopheles were active during the day, with increased day biting during the dry season. 3131 samples were identified morphologically as 14 different species, and a subset was also identified by DNA amplicon sequencing allowing determination of 29 Anopheles species. The investigation of well characterized insecticide mutations (ace-1, kdr, and rdl genes) indicated that individuals carried mutations associated with response to all the different classes of insecticides. There also appeared to be a non-random association between mosquito species and insecticide resistance with Anopheles peditaeniatus exhibiting nearly fixed mutations. Molecular screening for Plasmodium sp. presence indicated that 3.6% of collected Anopheles were positive, most for P. vivax followed by P. falciparum. These results highlight some of the key mechanisms driving residual human malaria transmission in Cambodia, and illustrate the importance of diverse collection methods, sites and seasons to avoid bias and better characterize Anopheles mosquito ecology in Southeast Asia.

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Section: Resultsmentioning

confidence: 99%

Section: Methodsmentioning

confidence: 99%

Section: Methodsmentioning

confidence: 99%

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Anopheles ecology, genetics and malaria transmission in northern Cambodia

Vantaux

Riehle

Piv

et al. 2021

Sci Rep

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“…The kdr mutation in the VGSC genes is one indicator of mosquito resistance to pyrethroid insecticides. 47,48 In malaria vector Anopheles mosquitoes, previous studies have found strong associations between kdr mutation frequency and phenotypic resistance status. 49,50 However, this association does not necessarily hold true for all mosquito species.…”

Section: Discussionmentioning

confidence: 99%

Widespread multiple insecticide resistance in the major dengue vectorAedes albopictusin Hainan Province, China

Zhou

Zhong

et al. 2021

Pest Management Science

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BACKGROUND Aedes albopictus is a highly invasive mosquito and has become a potential vector of dengue, chikungunya and Zika viruses. Insecticide‐based mosquito interventions are the main tools for vector‐borne disease control. However, mosquito resistance to insecticides is a major threat to effective prevention and control. Five Ae. albopictus populations across Hainan Province, China were investigated for susceptibility to multiple insecticide and resistance mechanisms. RESULTS Larval bioassays indicated that resistance to pyrethroids was common in all larval populations. Adult bioassays revealed all populations were either resistant or highly resistant to at least four of the six synthetic insecticides (deltamethrin, permethrin, cyfluthrin, propoxur, malathion, and DDT) tested. Pre‐exposure of mosquitoes to the synergistic agent piperonyl butoxide (PBO) increased mosquito mortality by 2.4–43.3% in bioassays to DDT, malathion, and permethrin and rendered mosquito sensitive to deltamethrin, cyfluthrin, and propoxur. The frequency of knockdown resistance (kdr) mutations (F1534S and F1534C) ranged from 69.8% to 89.3% and from 38.1% to 87.0% in field‐resistant and sensitive populations, respectively. F1534S mutation was significantly associated with pyrethroid resistance. No mutation was detected in the acetylcholinesterase (ace‐1) gene in the two examined populations. CONCLUSION This study provides evidence of widespread resistance to multiple insecticides in Ae. albopictus in Hainan Province, China. Both kdr mutations and metabolic detoxification were potential causes of insecticide resistance for Ae. albopictus. Our findings highlight the need for insecticide resistance management and mosquito control measures that do not entirely depend on synthetic insecticides. © 2020 The Authors. Pest Management Science published by John Wiley & Sons Ltd on behalf of Society of Chemical Industry.

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“…In An. sinensis , significant positive correlations have been found between kdr allele frequency and bioassay-based resistance phenotype, and three different mutations of VGSC at position 1014 (1014F/C/S) have been documented [ 20 , 21 , 24 – 28 ]. We found that all three mutations were present in Sichuan.…”

Section: Discussionmentioning

confidence: 99%

A survey of insecticide resistance-conferring mutations in multiple targets in Anopheles sinensis populations across Sichuan, China

Qian

Liu

Yang³

et al. 2021

Parasites Vectors

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Background Sichuan province is located in the southwest of China, and was previously a malaria-endemic region. Although no indigenous malaria case has been reported since 2011, the number of imported cases is on the rise. Insecticide-based vector control has played a central role in the prevention of malaria epidemics. However, the efficacy of this strategy is gravely challenged by the development of insecticide resistance. Regular monitoring of insecticide resistance is essential to inform evidence-based vector control. Unfortunately, almost no information is currently available on the status of insecticide resistance and associated mechanisms in Anopheles sinensis, the dominant malaria vector in Sichuan. In this study, efforts were invested in detecting the presence and frequency of insecticide resistance-associated mutations in three genes that encode target proteins of several classes of commonly used insecticides. Methods A total of 446 adults of An. sinensis, collected from 12 locations across Sichuan province of China, were inspected for resistance-conferring mutations in three genes that respectively encode acetylcholinesterase (AChE), voltage-gated sodium channel (VGSC), and GABA receptor (RDL) by DNA Sanger sequencing. Results The G119S mutation in AChE was detected at high frequencies (0.40–0.73). The predominant ace-1 genotype was GGC/AGC (119GS) heterozygotes. Diverse variations at codon 1014 were found in VGSC, leading to three different amino acid substitutions (L1014F/C/S). The 1014F was the predominant resistance allele and was distributed in all 12 populations at varying frequencies from 0.03 to 0.86. The A296S mutation in RDL was frequently present in Sichuan, with 296SS accounting for more than 80% of individuals in six of the 12 populations. Notably, in samples collected from Chengdu (DJY) and Deyang (DYMZ), almost 30% of individuals were found to be resistant homozygotes for all three targets. Conclusions Resistance-related mutations in three target proteins of the four main classes of insecticides were prevalent in most populations. This survey reveals a worrisome situation of multiple resistance genotypes in Sichuan malaria vector. The data strengthen the need for regular monitoring of insecticide resistance and establishing a region-customized vector intervention strategy.

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Resistance to pyrethroid and organophosphate insecticides, and the geographical distribution and polymorphisms of target-site mutations in voltage-gated sodium channel and acetylcholinesterase 1 genes in Anopheles sinensis populations in Shanghai, China

Cited by 25 publications

References 32 publications

Anopheles ecology, genetics and malaria transmission in northern Cambodia

Anopheles ecology, genetics and malaria transmission in northern Cambodia

Widespread multiple insecticide resistance in the major dengue vectorAedes albopictusin Hainan Province, China

A survey of insecticide resistance-conferring mutations in multiple targets in Anopheles sinensis populations across Sichuan, China

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