Resistance to lipopolysaccharide-induced endotoxic shock in heterozygous Zfp191 gene-knockout mice

Sun, Ruilin; Wang, H.Y.; Yang, Xiaosong; Zhao, S. J.; Li, L.M.; Wang, L.; Wang, Z.G.; Fei, J.

doi:10.4238/2011.december.8.6

Cited by 4 publications

(5 citation statements)

References 25 publications

(32 reference statements)

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“…For instance, deficiency of zDC in classic dendritic cells presented a higher inflammatory response to LPS (14). ZFP191 gene knockout mice were resistant to LPS-induced endotoxic shock (15). Recently, several studies with deficient mice of Gfi1, a C2H2 type transcription repressor, supported it as a negative regulator in immune response and inflammation (16 -18).…”

mentioning

confidence: 99%

Zinc Finger Protein 64 Promotes Toll-like Receptor-triggered Proinflammatory and Type I Interferon Production in Macrophages by Enhancing p65 Subunit Activation*

Wang

Liu

et al. 2013

Journal of Biological Chemistry

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Background:The role of ZFPs in innate immune responses remains unclear. Results: ZFP64 associates with the NF-B p65 subunit, enhancing promoter recruitment and activation and, thus, promoting TLR-triggered proinflammatory cytokine and IFN-␤ production. Conclusion: ZFP64 acts as a positive regulator of TLR signaling. Significance: The data provide mechanistic insights into the efficient activation of the TLR response.

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mentioning

confidence: 99%

Zinc Finger Protein 64 Promotes Toll-like Receptor-triggered Proinflammatory and Type I Interferon Production in Macrophages by Enhancing p65 Subunit Activation*

Wang

Liu

et al. 2013

Journal of Biological Chemistry

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“…To further investigate this relationship, we examined the effect of ZNF24 on pro-inflammatory cytokines. A study from Fei et al [ 48 ] has reported that knockdown of the mouse zinc finger protein Zfp 191 could reduce inflammatory cytokine levels during LPS stimulation. Similarly, we found that ZNF24 knockdown attenuated the upregulation of IL-6 and IL-1β induced by TNF-α treatment.…”

Section: Discussionmentioning

confidence: 99%

“…Oral mucositis caused by anticancer treatments was documented and evaluated according to the National Cancer Institute Common Terminology Criteria for Adverse Events 3.0 version (CTCAE 3.0) [ 48 ] weekly from the beginning of radiotherapy to one month after completion of treatment. The highest grade of oral mucositis that patients experienced during follow-up was recorded as their common toxicity criteria (CTC) scores.…”

Section: Methodsmentioning

confidence: 99%

Predictive single nucleotide polymorphism markers for acute oral mucositis in patients with nasopharyngeal carcinoma treated with radiotherapy

Niu

Chen

et al. 2017

Oncotarget

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The aim of this study was to investigate the association between the susceptibility of severe oral mucositis (OM) in Chinese nasopharyngeal carcinoma (NPC) patients treated with radiotherapy and single nucleotide polymorphisms (SNPs) across the whole genome. SNPs were screened in a total of 24 patients with NPC and an additional 6 were subjected to mRNA expression analysis. Patients were subdivided into CTC 0-2 (CTC toxicity grade 0, 1, and 2) and CTC 3+ (CTC toxicity grade 3 and above) groups according to their CTC (common toxicity criteria) scores. The GTEx dataset was used to performed eQTL analyses and in-vitro functional assays were performed for eQTL-associated genes. Our data identified 7 functional SNPs associated with the development of OM. We observed that rs11081899-A, located in the 5′-UTR of the ZNF24 gene, was significantly correlated with a higher risk of severe mucositis (OR = 14.631, 95% CI = 2.61-105.46, p = 1.2 × 10−4), and positively associated with ZNF24 mRNA expression (p = 4.1 × 10−6) from GTEx dataset. In addition, high ZNF24 mRNA expression was associated with severe OM in patients with NPC (p = 0.02). Further functional assays revealed that ZNF24 knockdown reduced p65 expression and suppressed TNF-α-induced NF-κB activation and pro-inflammatory cytokines release. These findings suggested that rs11081899-A may be a genetic susceptibility factor for radiation-induced OM in patients with NPC, although its value in clinical application needs to be further verified in a large cohort. Also, we suggested that downregulation of ZNF24 may attenuate the development of mucositis by suppressing NF-κB activation.

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“…Besides development, ZNF24 also participates in inflammation process. According to the data published by Sun et al,18 ZFP191(±) mice secreted less interleukin-1β and interleukin-6 upon lipopolysaccharide stimulation, thus resist to lipopolysaccharide-induced systemic inflammation and endotoxic shock. In addition, ZNF24 can promote cell migration of microvascular endothelial cells.…”

mentioning

confidence: 96%

Zinc Finger Protein 24 is a Prognostic Factor in Ovarian Serous Carcinoma

Chen

Guo

Yuan³

et al. 2021

Applied Immunohistochemistry &Amp; Molecular Morphology

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Objective: As a member of the zinc finger protein family, zinc finger protein 24 (ZNF24) contains a Cys2His2 zinc finger domain and acts as a transcription factor. ZNF24 has been reported to be downregulated in gastric cancer and breast cancer. However, little is known about its expression and function in ovarian serous carcinoma (OSC).Patients and Methods: We collected 117 OSC patients during 2011 to 2017 and retrospectively retrieved their clinicopathologic characteristics as well as their survival data. Protein level was analyzed by immunohistochemistry, mRNA level was evaluated by RT-qPCR assay, and transcriptional data was obtained from TCGA data sets. The correlations between ZNF24 expression and patients' features were assessed using χ 2 test. Univariate and multivariate analyses were used to identify the prognosis predicative potential of ZNF24 in OSC. The function of ZNF24 in the epithelial ovarian cancer cells was also verified by in vitro cellular experiments.Results: Among the 117 cases, ZNF24 was downregulated in 52 OSC samples (44.6%) and significantly correlated with tumor stages. According to univariate and multivariate analyses, ZNF24 can act as an independent prognostic indicator for the overall survival of OSC patients, whose lower expression was associated with poorer clinical outcomes. Ectopic overexpression and knockdown assays indicated that ZNF24 can negatively regulate the OSC cell viability.Conclusions: OSC patients with low level of ZNF24 have worse overall survival compared with those possess high-ZNF24 expression. Downregulated ZNF24 may be involved in the proliferation of OSC, and ZNF24 expression can serve as an independent survival predictor.

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Resistance to lipopolysaccharide-induced endotoxic shock in heterozygous Zfp191 gene-knockout mice

Cited by 4 publications

References 25 publications

Zinc Finger Protein 64 Promotes Toll-like Receptor-triggered Proinflammatory and Type I Interferon Production in Macrophages by Enhancing p65 Subunit Activation*

Zinc Finger Protein 64 Promotes Toll-like Receptor-triggered Proinflammatory and Type I Interferon Production in Macrophages by Enhancing p65 Subunit Activation*

Predictive single nucleotide polymorphism markers for acute oral mucositis in patients with nasopharyngeal carcinoma treated with radiotherapy

Zinc Finger Protein 24 is a Prognostic Factor in Ovarian Serous Carcinoma

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