Resistance to -lactams of human and veterinary Salmonella isolates in Egypt and Algeria

Abstract

Seventy six non-typhoid Salmonella were isolated from both human and poultry in Egypt and Algeria and tested for their antibiotics resistance. The incidence of multiple antibiotics resistance was high. To study -lactams resistance mechanisms, double disk synergy test (DDST) with and without cloxacilline was used, results revealed the production of extended spectrum -lactamases (ESBLs) and cephalosporinase in seven and one human Egyptian isolates, respectively. The seven ESBL isolates were identified as Salmonella enterica serotype Poona and their molecular typing by ERIC-PCR revealed unrelated genetic patterns, indicating that these isolates are not clonal. The Cephalosporinase-ESBL-producing isolate was identified as S. enterica serotype Hadar. Polymerase chain reaction (PCR) with specific primers showed the presence of bla TEM and bla SHV genes, respectively, in all and four ESBL producers, and bla AmpC gene was detected in cephalosporinaseproducing isolate. Genetic transfer by conjugation and plasmid profiles analysis showed that these genes and their resistance markers were transferable in association with plasmids of 60 kb for ESBLs and 64 and 3.2 kb for AmpC cephalosporinase.

Isolation and Characterization of Two Lytic Bacteriophages Infecting a Multi-Drug Resistant Salmonella Typhimurium and Their Efficacy to Combat Salmonellosis in Ready-to-Use Foods

“…MDR Salmonella serovars were previously isolated from Egypt [ 65 , 66 , 67 , 68 , 69 ]. Traditional intervention methods for combating Salmonella have not been able to solve the dilemma of food quality and food safety [ 14 , 15 , 16 , 17 ].…”

Application of a novel lytic Jerseyvirus phage LPSent1 for the biological control of the multidrug-resistant Salmonella Enteritidis in foods

“…Remarkably, all the tested bacteria were identified as MDR because they resisted at least one antibiotic within more than three different classes. MDR Salmonella have been isolated previously from Egypt ( Abdelhakim et al, 2011 ; Abdel-Maksoud et al, 2015 ; Mahmoud et al, 2018 ; Merwad and Abdel-Haliem, 2018 ; Esmael et al, 2021b ; Abd-Elghany et al, 2022 ). In Egypt there are no boundaries on antibiotic remedies and antibiotics can be acquired easily without any medical prescription from drugstores and pharmacies ( Sobhy et al, 2012 ; Sabry et al, 2014 ; Awad et al, 2016 ; Esmael et al, 2020 ).…”

Salmonellosis: A food borne zoonotic and public health disease in Egypt

“…Similarly, high prevalence of AMR genes of non-typhoid Salmonella isolates to tetracycline and chloramphenicol was also detected [55]. A total of 76 non-typhoid Salmonellae were isolated from human and poultry in Egypt and Algeria and the isolates showed high incidence of cephalosporin's resistance [56]. A recent work of Eissa et al [12] demonstrated that patients with typhoid fever showed resistance to combined treatment with ciprofloxacin and Cefotax.…”