Resistance to Fusarium oxysporum f. sp. gladioli in transgenic Gladiolus plants expressing either a bacterial chloroperoxidase or fungal chitinase genes

Kamo, Kathryn; Lakshman, Dilip K.; Pandey, R. K.; Guaragna, M. A.; Okubara, Patricia A.; Rajasekaran, Kanniah; Cary, Jeffrey W.; Jordan, R.

doi:10.1007/s11240-015-0913-1

Cited by 9 publications

(3 citation statements)

References 44 publications

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“…On the other hand, Refs. [194][195][196]200,201] have conducted genetic transformation research and created a number of transgenic gladiolus plants using agrobacterium-based techniques. Gladiolus has not been reported to use the CRISPR-Cas system, despite its importance in the development of desirable plants nowadays.…”

Section: Insights and New Technologies For In Vitro Culture Of Gladiolusmentioning

confidence: 99%

In Vitro Propagation Journey of Ornamental Gladiolus (Gladiolus Species): A Systematic Review Analysis Based on More Than 50 Years Research

Kumar,

Chaudhary,

Sirohi

et al. 2024

Horticulturae

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Traditional gladiolus propagation methods are now supplemented with in vitro propagation to meet the demands of modern floriculture in terms of quick production of disease-free, quality planting material. Due to virus infections, vegetative propagation in gladiolus in the field is slow, and is a serious concern in the propagation of gladiolus. In vitro propagation provides an enormous increase in propagation rate and the ability to produce disease-free plant material. Numerous elements, including cultivars, explant type, size of explants, position of explants on medium, plant growth regulators and certain additives, incubation conditions, and sub-culturing time, all have a significant impact on in vitro clonal propagation of gladiolus plants as well as the development of in vitro cormel efficiency. There are certain obstacles and challenges that arise in the in vitro development of plants and the cormels of gladiolus. However, numerous studies and review reports on gladiolus for in vitro propagation have been reported, but very little is known about the factors influencing gladiolus’ in vitro effectiveness. In the present review, we focused on and analyzed research data accumulated over 50 years on diverse strategies for in vitro propagation such as direct, indirect organogenesis, and somatic embryogenesis, as well as various factors such as physical, nutritional, and hormonal influences on in vitro propagation, in vitro cormel formation efficiency, difficulties that arise, and new insights into in vitro development in gladiolus from the available literature worldwide. Future possibilities for further improvement in the in vitro propagation of ornamental gladiolus are also discussed. The current review provides insight into a comprehensive protocol for gladiolus in vitro propagation and emphasizes the importance of continuously advancing tissue culture techniques and factors influencing the in vitro efficiency towards improving in vitro plantlets and cormels in gladiolus (Gladiolus spp.).

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Section: Insights and New Technologies For In Vitro Culture Of Gladiolusmentioning

confidence: 99%

In Vitro Propagation Journey of Ornamental Gladiolus (Gladiolus Species): A Systematic Review Analysis Based on More Than 50 Years Research

Kumar,

Chaudhary,

Sirohi

et al. 2024

Horticulturae

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“…Transgenic gladiolus 'Peter Pears', developed by transforming with a synthetic antimicrobial peptide gene (D4E1), exhibited enhanced resistance to F.oxysporum [131]. Kamo et al (2016) demonstrated that cell extracts from the transgenic gladiolus, overexpressing a fungal exochitinase, endochitinase, or a bacterial chloroperoxidase, could inhibit the growth of F. oxysporum [132]. The Ace-AMP1 gene was transformed in scented geraniums to provide resistance to B. cinerea, and the expression level of the Ace-AMP1 protein was proportionally correlated with enhanced resistance to Botrytis sporulation [133].…”

Section: Other Ornamentalsmentioning

confidence: 99%

Recent Progress in Enhancing Fungal Disease Resistance in Ornamental Plants

Mekapogu

Jung

Kwon

et al. 2021

IJMS

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Fungal diseases pose a major threat to ornamental plants, with an increasing percentage of pathogen-driven host losses. In ornamental plants, management of the majority of fungal diseases primarily depends upon chemical control methods that are often non-specific. Host basal resistance, which is deficient in many ornamental plants, plays a key role in combating diseases. Despite their economic importance, conventional and molecular breeding approaches in ornamental plants to facilitate disease resistance are lagging, and this is predominantly due to their complex genomes, limited availability of gene pools, and degree of heterozygosity. Although genetic engineering in ornamental plants offers feasible methods to overcome the intrinsic barriers of classical breeding, achievements have mainly been reported only in regard to the modification of floral attributes in ornamentals. The unavailability of transformation protocols and candidate gene resources for several ornamental crops presents an obstacle for tackling the functional studies on disease resistance. Recently, multiomics technologies, in combination with genome editing tools, have provided shortcuts to examine the molecular and genetic regulatory mechanisms underlying fungal disease resistance, ultimately leading to the subsequent advances in the development of novel cultivars with desired fungal disease-resistant traits, in ornamental crops. Although fungal diseases constitute the majority of ornamental plant diseases, a comprehensive overview of this highly important fungal disease resistance seems to be insufficient in the field of ornamental horticulture. Hence, in this review, we highlight the representative mechanisms of the fungal infection-related resistance to pathogens in plants, with a focus on ornamental crops. Recent progress in molecular breeding, genetic engineering strategies, and RNAi technologies, such as HIGS and SIGS for the enhancement of fungal disease resistance in various important ornamental crops, is also described.

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“…For conferring resistance to Fusarium oxysporum f. sp. gladioli causing fusarium wilt, which is the major devastating disease in gladiolus, Kamo et al 75 transformed gladiolus cv. Peter Pears with three antifungal genes, a non-heme chloroperoxidase from Pseudomonas pyrrocinia, and an exochitinase and endochitinase from Fusarium venetanum under CaMV 35S promoter.…”

Section: Gladiolus (Gladiolus Grandiflorus L)mentioning

confidence: 99%

Transgenics in Ornamental Crops:Creating Novelties in Economically Important Cut Flowers

Sharma

Messar

2017

Current Science

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Development of transgenics is the need of the modern era of plant breeding, as they possess the potential to incorporate those characters in crop varieties which are either difficult or impossible through conventional breeding approaches. In case of ornamental crops, the progress made in transgenic breeding is not that impressive like in cereals, pulses and vegetables, but the initiatives taken and advancements made have implicated the bright future of this technology in ornamental crops. Improved morphology, flower colour, resistance and fragrance are some of the desired novel traits in ornamental crops where transgenic approaches need to intervene. Transgenic breeding in major cut-flower crops like rose, chrysanthemum, gladiolus and carnation has provided avenues for incorporation of novel traits in other ornamental crops as well and has made such crops an ideal target for application of other advanced technologies.

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Resistance to Fusarium oxysporum f. sp. gladioli in transgenic Gladiolus plants expressing either a bacterial chloroperoxidase or fungal chitinase genes

Cited by 9 publications

References 44 publications

In Vitro Propagation Journey of Ornamental Gladiolus (Gladiolus Species): A Systematic Review Analysis Based on More Than 50 Years Research

In Vitro Propagation Journey of Ornamental Gladiolus (Gladiolus Species): A Systematic Review Analysis Based on More Than 50 Years Research

Recent Progress in Enhancing Fungal Disease Resistance in Ornamental Plants

Transgenics in Ornamental Crops:Creating Novelties in Economically Important Cut Flowers

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