Resistance to antimicrobial agents among enterococci isolated from fecal samples of wild marine species in the southern coast of Brazil

Prichula, Janira; Pereira, Rebeca Inhoque; Wachholz, Guilherme Raffo; Cardoso, Leonardo Almansa; Tolfo, Neidimar Cezar Correa; Santestevan, Naiara Aguiar; Medeiros, Aline Weber; Tavares, Maurício; Frazzon, Jeverson; d’Azevedo, Pedro Alves; Frazzon, Ana Paula Guedes

doi:10.1016/j.marpolbul.2016.02.071

Cited by 43 publications

(50 citation statements)

References 36 publications

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“…All strains were already identified as genus, species and virulence profile in previous studies Riboldi et al 2009;Cassenego et al, 2013;Prichula et al, 2013;Santestevan et al, 2015;Prichula et al, 2016). The strains were preserved in 10% (w/v) skim milk (Difco, Sparks, MD, USA) solution supplemented with 10% (v/v) glycerol (LabSynth  ) frozen at -20 °C.…”

Section: Enterococcus Strainsmentioning

confidence: 99%

“…For DNA isolation, an aliquot of frozen bacterial cells was recovered in Brain Heart Infusion agar (BHA, Himedia  , India), and incubated at 35 °C for 24 h. Genomic DNA was extracted using the method described by Donato (2007). All strains were confirmed to genus and species by polymerase chain reaction (PCR) assays according to the protocol established by Prichula et al (2016). The primers sequences and their amplification products are listed in Table 2.…”

Section: Dna Isolationmentioning

confidence: 99%

“…Enterococci are important Gram-positive bacteria recognized as part of gut microbiota in humans and animals, and they are widely distributed in soil, water, plants and foods (Cassenego et al, 2013;Lebreton et al, 2014;Pieniz et al, 2015;Santestevan et al, 2015;Prichula et al, 2016;Medeiros et al, 2017). Their ubiquity reflects its ability to survive in a variety of environmental stressors (Byappanahalli et al, 2012).…”

Section: Introductionmentioning

confidence: 99%

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Frequency of Clustered Regularly Interspaced Short Palindromic Repeats (CRISPRs) in non-clinical Enterococcus faecalis and Enterococcus faecium strains

et al. 2019

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The fidelity of the genomes is defended by mechanism known as Clustered Regularly Interspaced Short Palindromic Repeats (CRISPR) systems. Three Type II CRISPR systems (CRISPR1- cas, CRISPR2 and CRISPR3-cas) have been identified in enterococci isolates from clinical and environmental samples. The aim of this study was to observe the distribution of CRISPR1-cas, CRISPR2 and CRISPR3-cas in non-clinical strains of Enterococcus faecalis and Enterococcus faecium isolates from food and fecal samples, including wild marine animals. The presence of CRISPRs was evaluated by PCR in 120 enterococci strains, 67 E. faecalis and 53 E. faecium. It is the first report of the presence of the CRISPRs system in E. faecalis and E. faecium strains isolated from wild marine animal fecal samples. The results showed that in non-clinical strains, the CRISPRs were more frequently detected in E. faecalis than in E. faecium. And the frequencies of CRISPR1-cas and CRISPR2 were higher (60%) in E. faecalis strains isolated from animal feces, compared to food samples. Both strains showed low frequencies of CRISPR3-cas (8.95% and 1.88%). In conclusion, the differences in the habitats of enterococcal species may be related with the results observe in distribution of CRISPRs systems.

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Section: Enterococcus Strainsmentioning

confidence: 99%

Section: Dna Isolationmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

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Frequency of Clustered Regularly Interspaced Short Palindromic Repeats (CRISPRs) in non-clinical Enterococcus faecalis and Enterococcus faecium strains

et al. 2019

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“…Although aquatic environments are characterized by low bacterial concentrations in respect to others, such as soil or animals, several studies have demonstrated an increase of resistance traits in these environments as well [4]. Thus, marine animals, which are completely immersed in their environment, can be environmental health indicators, reservoirs and spreaders of ABR strains [5][6][7][8][9][10]. Additionally, the isolation of ABR strains can be considered as a spoiling index of the habitat of these animals [11,12].…”

Section: Introductionmentioning

confidence: 99%

Antibiotic Resistance of Gram-Negative Bacteria from Wild Captured Loggerhead Sea Turtles

Blasi¹,

Migliore

Mattei³

et al. 2020

Antibiotics

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Sea turtles have been proposed as health indicators of marine habitats and carriers of antibiotic-resistant bacterial strains, for their longevity and migratory lifestyle. Up to now, a few studies evaluated the antibacterial resistant flora of Mediterranean loggerhead sea turtles (Caretta caretta) and most of them were carried out on stranded or recovered animals. In this study, the isolation and the antibiotic resistance profile of 90 Gram negative bacteria from cloacal swabs of 33 Mediterranean wild captured loggerhead sea turtles are described. Among sea turtles found in their foraging sites, 23 were in good health and 10 needed recovery for different health problems (hereafter named weak). Isolated cloacal bacteria belonged mainly to Enterobacteriaceae (59%), Shewanellaceae (31%) and Vibrionaceae families (5%). Although slight differences in the bacterial composition, healthy and weak sea turtles shared antibiotic-resistant strains. In total, 74 strains were endowed with one or multi resistance (up to five different drugs) phenotypes, mainly towards ampicillin (~70%) or sulfamethoxazole/trimethoprim (more than 30%). Hence, our results confirmed the presence of antibiotic-resistant strains also in healthy marine animals and the role of the loggerhead sea turtles in spreading antibiotic-resistant bacteria.

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“…Previously only a small number of studies have investigated the presence of resistant bacteria in wild marine species (Johnson and others 1998, Thornton and others 1998, Lisle and others 2004, Lockwood and others 2006, Rose and others 2009, Barros and others 2011, Marinho and others 2013, Stewart and others 2014, Santestevan and others 2015, Prichula and others 2016). This could be because of the difficulty in obtaining samples from wild marine animals due to the migratory habits of many species.…”

mentioning

confidence: 99%

Antibiotic‐resistant bacteria in free‐living marine species

Frazzon

2016

Veterinary Record

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Resistance to antimicrobial agents among enterococci isolated from fecal samples of wild marine species in the southern coast of Brazil

Cited by 43 publications

References 36 publications

Frequency of Clustered Regularly Interspaced Short Palindromic Repeats (CRISPRs) in non-clinical Enterococcus faecalis and Enterococcus faecium strains

Frequency of Clustered Regularly Interspaced Short Palindromic Repeats (CRISPRs) in non-clinical Enterococcus faecalis and Enterococcus faecium strains

Antibiotic Resistance of Gram-Negative Bacteria from Wild Captured Loggerhead Sea Turtles

Antibiotic‐resistant bacteria in free‐living marine species

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