Resistance screening against Cucumber vein yellowing virus using a real-time (Taqman®) RT-PCR assay in cucumber (Cucumis sativus)

Gil-Salas, Francisco M.; Colyer, Alison; Boonham, Neil; Cuadrado, I. M.; Janssen, Dirk

doi:10.1016/j.cropro.2008.09.005

Cited by 18 publications

(12 citation statements)

References 24 publications

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“…The high transmission efficiency of CVYV and the difficulty of controlling the whitefly B. tabaci made it necessary to search for genetic resistance or tolerance to the virus. Several cucumber varieties with different levels of resistance against CVYV European isolates were developed by seed companies but screening assays for virus resistance have been very scarce, and the genetic or biological variations of CVYV were not taken into account (Gil-Salas et al, 2009). Recently, a CVYV isolate from Jordan (isolate Jor) has been characterized that induced severe disease symptoms on all resistant cucumber varieties tested (Galipienso et al, 2012(Galipienso et al, , 2013.…”

Section: Introductionmentioning

confidence: 99%

Genetic variation and evolutionary forces shaping Cucumber vein yellowing virus populations: risk of emergence of virulent isolates in Europe

et al. 2015

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The genetic variation and evolutionary mechanisms shaping Cucumber vein yellowing virus (CVYV) populations were investigated by analysis of nucleotide sequences coding for P1b, P1b/P3 and coat proteins (CP) from isolates collected in different countries. The complete genome sequence of isolate ISM from Israel was also determined and compared to those of isolates Jor from Jordan and ALM32 from Spain. This isolate had overall nucleotide identities of 94·23 and 94·96% with ALM32 and Jor, respectively. Nucleotide variation among isolates was not homogeneously distributed, with the 5′ half of the genome being more variable than the 3′ half. A Bayesian phylogenetic tree of the CP showed that CVYV isolates clustered into two main clades: isolates from the Middle East region (Lebanon, Israel and Jordan) clustered in both clades whereas the isolate from Tunisia clustered in clade I and the European isolates clustered as a homogeneous phylogroup in Clade II. A similar topology was observed for P1b but with incongruences with respect to the CP, suggesting genetic exchange among virus isolates, which were confirmed with recombination algorithms. The low genetic diversity within the European phylogroup with respect to the other isolates, neutralist tests and genetic differentiation analyses suggest that the Middle East region is the cradle of CVYV and that a unique virus introduction event occurred in Europe, where the virus spread rapidly. Taken together, these findings indicate a risk of emergence of virulent CVYV isolates in Europe either through migration from the Middle East or by genetic changes of the European isolates.

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Section: Introductionmentioning

confidence: 99%

Genetic variation and evolutionary forces shaping Cucumber vein yellowing virus populations: risk of emergence of virulent isolates in Europe

et al. 2015

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“…Viral diseases of cucurbit crops are major limiting factors for their production and may cause massive damage, particularly to melon and squash (Lovisolo, 1980), resulting in huge economic losses (Gaba et al ., 2004). Major viruses that infect melon include: Cucurbit aphid‐borne yellows virus (CABYV, genus Polerovirus ), Watermelon mosaic virus (WMV, genus Potyvirus ), Zucchini yellow mosaic virus (ZYMV, genus Potyvirus ), Papaya ringspot virus (PRSV, genus Potyvirus ), Moroccan watermelon mosaic virus (MWMV, genus Potyvirus ) and Cucumber mosaic virus (CMV, genus Cucumovirus ), which are transmitted by aphids; Cucurbit yellow stunting disorder virus (CYSDV, genus Crinivirus ), Beet pseudoyellows virus (BPYV, genus Closterovirus ) and Cucumber vein yellowing virus (CVYV, genus Ipomovirus ), which are transmitted by whiteflies (Abou‐Jawdah et al ., 2000; Bananej and Vahdat, 2008; Gil‐Salas et al ., 2009; Kassem et al ., 2007; Lecoq et al ., 2003, 2011; Papayiannis et al ., 2005; Sevik and Arli‐Sokmen, 2003; Yakoubi et al ., 2007); and Melon necrotic spot virus (MNSV, genus Carmovirus ), which is transmitted by the soil fungus Olpidium bornovanus (Bananej and Vahdat, 2008; Cuadrado et al ., 1993).…”

Section: Introductionmentioning

confidence: 99%

Melon RNA interference (RNAi) lines silenced for Cm‐eIF4E show broad virus resistance

Rodríguez-Hernández

Gosálvez

Sempere

et al. 2012

Molecular Plant Pathology

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Efficient and sustainable control of plant viruses may be achieved using genetically resistant crop varieties, although resistance genes are not always available for each pathogen; in this regard, the identification of new genes that are able to confer broad-spectrum and durable resistance is highly desirable. Recently, the cloning and characterization of recessive resistance genes from different plant species has pointed towards eukaryotic translation initiation factors (eIF) of the 4E family as factors required for the multiplication of many different viruses. Thus, we hypothesized that eIF4E may control the susceptibility of melon (Cucumis melo L.) to a broad range of viruses. To test this hypothesis, Cm-eIF4E knockdown melon plants were generated by the transformation of explants with a construct that was designed to induce the silencing of this gene, and the plants from T2 generations were genetically and phenotypically characterized. In transformed plants, Cm-eIF4E was specifically silenced, as identified by the decreased accumulation of Cm-eIF4E mRNA and the appearance of small interfering RNAs derived from the transgene, whereas the Cm-eIF(iso)4E mRNA levels remained unaffected. We challenged these transgenic melon plants with eight agronomically important melon-infecting viruses, and identified that they were resistant to Cucumber vein yellowing virus (CVYV), Melon necrotic spot virus (MNSV), Moroccan watermelon mosaic virus (MWMV) and Zucchini yellow mosaic virus (ZYMV), indicating that Cm-eIF4E controls melon susceptibility to these four viruses. Therefore, Cm-eIF4E is an efficient target for the identification of new resistance alleles able to confer broad-spectrum virus resistance in melon.

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“…The mixed solution was extracted absolutely in ultrasonic instrument (KQ 5200E, Jiangsu, China) at 4 °C for 1 h, and centrifuged at 10 000 r/min under 4 °C for 20 min. Supernatant was placed in a 50-ml test tube, recentrifuged Gil-Salas et al (2009) at 10 000 r/min under 4 °C for 20 min. The aqueous phase was discarded and the chloroform phase was put into a 10-ml tube.…”

Section: Analysis Of Phospholipids By High Performance Liquid Chromatmentioning

confidence: 99%

Responses of phospholipase D and lipoxygenase to mechanical wounding in postharvest cucumber fruits

Zhao

Qian

Chen

et al. 2010

J. Zhejiang Univ. Sci. B

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Abstract:This study was to investigate the responses of phospholipase D (PLD) and lipoxygenase (LOX) to mechanical wounding in postharvest cucumber (Cucumis sativus L. cv. Biyu-2) fruits. Membrane-associated Ca 2+ content, activities and gene expression of PLD and LOX, and contents of phosphatidylcholine (PC), phosphatidylinositol (PI), and phosphatidic acid (PA) were determined in cucumber fruits following mechanical wounding. Results show that PLD and LOX activities increased with the PLD and LOX mRNAs which are upregulated upon wounding, while membrane-associated Ca 2+ content decreased. Accompanying with the increase of PLD and LOX activities, accumulation of PA and losses of PC and PI were observed in all fruits, but there were differences of degrees between wounded and control fruits. Results suggest that PLD and LOX might be the main hydrolytic enzymes of phospholipids in postharvest cucumber fruits participating in the mechanical wounding injury. The activation of PLD and LOX might be the result of gene expression, which could be stimulated by the Ca 2+ flowing from the membrane to the cytoplasm upon receiving the wounding signals.

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Resistance screening against Cucumber vein yellowing virus using a real-time (Taqman®) RT-PCR assay in cucumber (Cucumis sativus)

Cited by 18 publications

References 24 publications

Genetic variation and evolutionary forces shaping Cucumber vein yellowing virus populations: risk of emergence of virulent isolates in Europe

Genetic variation and evolutionary forces shaping Cucumber vein yellowing virus populations: risk of emergence of virulent isolates in Europe

Melon RNA interference (RNAi) lines silenced for Cm‐eIF4E show broad virus resistance

Responses of phospholipase D and lipoxygenase to mechanical wounding in postharvest cucumber fruits

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