Resistance Profile Survey of 50 Periodontal Strains of <i>Actinobacillus actinomycetemcomitans</i>

Madinier, I.; Fosse, T.; Hitzig, C; Charbit, Yves; Hannoun, L.

doi:10.1902/jop.1999.70.8.888

Cited by 39 publications

(26 citation statements)

References 39 publications

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“…Also, the constant emergence of resistant strains makes it difficult to treat A. actinomycetemcomitans infections, resulting in a greater number of cases that recur following treatment. Various chemical agents have been evaluated over the years for their antimicrobial effects in the oral cavity; however, all are associated with side effects that prohibit regular long-term use (6,20,36,41,55,57,61,75). Therefore, an alternative therapy is essential to prevent the disease.…”

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confidence: 99%

In Vitro Efficacy of Diallyl Sulfides against the Periodontopathogen Aggregatibacter actinomycetemcomitans

Velliyagounder

Ganeshnarayan

Velusamy

et al. 2012

Antimicrob Agents Chemother

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The in vitro antibacterial effects of diallyl sulfide (DAS) against the Gram-negative periodontopathogen Aggregatibacter actinomycetemcomitans, the key etiologic agent of the severe form of localized aggressive periodontitis and other nonoral infections, were studied. A. actinomycetemcomitans was treated with garlic extract, allicin, or DAS, and the anti-A. actinomycetemcomitans effects of the treatment were evaluated. Garlic extract, allicin, and DAS significantly inhibited the growth of A. actinomycetemcomitans (greater than 3 log; P < 0.01) compared to control cells. Heat inactivation of the garlic extracts significantly reduced the protein concentration; however, the antimicrobial effect was retained. Purified proteins from garlic extract did not exhibit antimicrobial activity. Allicin lost all its antimicrobial effect when it was subjected to heat treatment, whereas DAS demonstrated an antimicrobial effect similar to that of the garlic extract, suggesting that the antimicrobial activity of garlic extract is mainly due to DAS. An A. actinomycetemcomitans biofilm-killing assay performed with DAS showed a significant reduction in biofilm cell numbers, as evidenced by both confocal microscopy and culture. Scanning electron microscopy (SEM) analysis of DAS-treated A. actinomycetemcomitans biofilms showed alterations of colony architecture indicating severe stress. Flow cytometry analysis of OBA9 cells did not demonstrate apoptosis or cell cycle arrest at therapeutic concentrations of DAS (0.01 and 0.1 g/ml). DAStreated A. actinomycetemcomitans cells demonstrated complete inhibition of glutathione (GSH) S-transferase (GST) activity. However, OBA9 cells, when exposed to DAS at similar concentrations, showed no significant differences in GST activity, suggesting that DAS-induced GST inhibition might be involved in A. actinomycetemcomitans cell death. These findings demonstrate that DAS exhibits significant antibacterial activity against A. actinomycetemcomitans and that this property might be utilized for exploring its therapeutic potential in treatment of A. actinomycetemcomitans-associated oral and nonoral infections.

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confidence: 99%

In Vitro Efficacy of Diallyl Sulfides against the Periodontopathogen Aggregatibacter actinomycetemcomitans

Velliyagounder

Ganeshnarayan

Velusamy

et al. 2012

Antimicrob Agents Chemother

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“…Greater resistance against the antibiotics clindamycin, metronidazole, and amoxicillin has been reported in P. gingivalis and A. actinomycetemcomitans associated with periodontal disease (Ardila et al 2010). Significantly, higher incidence of resistance against spiramycin and metronidazole has been reported in periodontal A. actinomycetemcomitans strains (Madinier 1999). Even oral bacteria that are not directly involved in dental and/or periodontal diseases exhibit resistant to antimicrobial agents (Kouidhi et al 2011;Villedieu et al 2004).…”

Section: Resistance To Antimicrobial Agents Among Oral Bacteriamentioning

confidence: 99%

ZnO and TiO2 nanoparticles as novel antimicrobial agents for oral hygiene: a review

Khan

Al-Khedhairy

Musarrat³

2015

J Nanopart Res

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Oral cavity is inhabited by more than 25,000 different bacterial phylotypes; some of them cause systemic infections in addition to dental and periodontal diseases. Emergence of multiple antibiotic resistance among these bacteria necessitates the development of alternative antimicrobial agents that are safe, stable, and relatively economic. This review focuses on the significance of metal oxide nanoparticles, especially zinc oxide and titanium dioxide nanoparticles as supplementary antimicrobials for controlling oral infections and biofilm formation. Indeed, the ZnO NPs and TiO 2 NPs have exhibited significant antimicrobial activity against oral bacteria at concentrations which is not toxic in in vivo toxicity assays. These nanoparticles are being produced at an industrial scale for use in a variety of commercial products including food products. Thus, the application of ZnO and TiO 2 NPs as nanoantibiotics for the development of mouthwashes, dental pastes, and other oral hygiene materials is envisaged. It is also suggested that these NPs could serve as healthier, innocuous, and effective alternative for controlling both the dental biofilms and oral planktonic bacteria with lesser side effects and antibiotic resistance.

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“…47,48 Further studies characterized the b-lactamases produced by oral bacteria, mainly in Bacteroidaceae, since members of this family were implicated in the aetiology of acute oral infections and periodontal disease. 49,50 More recently, the b-lactamase genes in subgingival oral bacteria, which were isolated from patients with refractory periodontitis, were characterized. 51,52 The CfxA b-lactamase gene was shown to be prevalent in the Prevotella and Capnocytophaga species.…”

Section: B-lactamasesmentioning

confidence: 99%

Bacterial resistance and the dental professionals’ role to halt the problem

Al‐Haroni

2008

Journal of Dentistry

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Resistance Profile Survey of 50 Periodontal Strains of Actinobacillus actinomycetemcomitans

Cited by 39 publications

References 39 publications

In Vitro Efficacy of Diallyl Sulfides against the Periodontopathogen Aggregatibacter actinomycetemcomitans

In Vitro Efficacy of Diallyl Sulfides against the Periodontopathogen Aggregatibacter actinomycetemcomitans

ZnO and TiO2 nanoparticles as novel antimicrobial agents for oral hygiene: a review

Bacterial resistance and the dental professionals’ role to halt the problem

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