Resistance Plasmid Families in
            <i>Enterobacteriaceae</i>

Carattoli, Alessandra

doi:10.1128/aac.01707-08

Cited by 1,068 publications

(958 citation statements)

References 122 publications

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“…Even so, reports have indicated that bla CTX-M-15 -encoded enzymes show more activity against ceftazidime than do other bla CTX-M gene derivatives and that point mutations in the active site of CTX-M-type b-lactamases can increase their activity against this b-lactam (Mushtaq et al, 2003). The bla CTX-M-15 gene has been reported to coexist on the same plasmid with the bla TEM-1 gene (Carattoli, 2009); this correlates well with the results of the current study, which showed that all the isolates that possessed the bla TEM-1 gene also possessed the bla CTX-M 15 gene (with the exception of the single nalidixic acidresistant isolate, strain 5; see Table 1). Only one of the ESBL-producers from this study was PCR-positive for the SHV-type b-lactamase, which is encoded by the bla SHV-2 gene.…”

Section: Jmm Correspondencesupporting

confidence: 80%

“…In members of the family Enterobacteriaceae, extended-spectrum blactamase (ESBL) and AmpC b-lactamase production are important mechanisms of resistance to b-lactam antimicrobials such as ceftriaxone, ceftazidime and cefotaxime (Kruger et al, 2004). ESBL and AmpC b-lactamase production is often associated with resistance to other classes of antimicrobials and, as a result, antimicrobial treatment of ESBLproducing isolates becomes limited (Carattoli, 2009). Dysentery caused by ESBL-and AmpC b-lactamase-producing species of the genus Shigella is seen relatively infrequently (Vinh et al, 2009).…”

Section: Jmm Correspondencementioning

confidence: 99%

“…The majority of the ESBL-producing isolates harboured the bla CTX-M-15 gene, which is carried on plasmids and can be disseminated via horizontal gene transfer to other Gramnegative pathogens (Carattoli, 2009;Liebana et al, 2006). In the current study, a series of ESBL-producing Shigella isolates from South Africa have been identified, which, although comprising ,1 % of all the isolates obtained for surveillance, are clinically relevant as they represent a challenge in disease management and patient treatment.…”

Section: Jmm Correspondencementioning

confidence: 99%

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Molecular characterization of extended-spectrum β-lactamase-producing Shigella isolates from humans in South Africa, 2003–2009

Tau¹,

Smith

Sooka³

et al. 2012

Journal of Medical Microbiology

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Section: Jmm Correspondencesupporting

confidence: 80%

Section: Jmm Correspondencementioning

confidence: 99%

Section: Jmm Correspondencementioning

confidence: 99%

See 1 more Smart Citation

Molecular characterization of extended-spectrum β-lactamase-producing Shigella isolates from humans in South Africa, 2003–2009

Tau¹,

Smith

Sooka³

et al. 2012

Journal of Medical Microbiology

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“…This result is in accordant with the results being reported in Carvalho et al (2011). Reports hypothesized that the versatility of plasmids together with the abuse of antimicrobials in human medicine may have largely contributed to the spread of antimicrobial resistance worldwide (Carattoli, 2009). In the same manner, piperacillin-tazobactam, ampicillin-sulbactam and amikacin were the most active drug after carbapenem, study results similar to report from Iran, Feizabadi et al (2008) found that 42.1 and 38.2% of carbapenem resistant A. baumannii were susceptible to piperacillin-tazobactam and amikacin, respectively.…”

Section: Discussionsupporting

confidence: 82%

First Report of the blaOXA-23 Gene in a Clinical Isolates of Acinetobacter baumannii in Najaf Hospitals-Iraq

Alsehlawi¹,

Alshara²,

Hadi³

et al. 2015

Research J. of Microbiology

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Carbapenemase producing Acinetobacter baumannii is frequently associated with nosocomial infections. Increasing resistance to carbapenems, may significantly reduce the choice of effective antibiotics. This study was conducted to determine the occurrence of carbapenemase producing A. baumannii isolates obtained from Najaf hospitals. Isolates were identified according to API 20NE system and more confirmed using 16Sr RNA gene. Carbapenem susceptibility was assayed by microbroth dilution and other antibiotics using disks diffusion test. Phenotypic detection of carbapenemase was performed using the imipenem-EDTA disk and modified Hodg tests. Then isolates were subjected to monoplex PCR targeting blaOXA-23, blaIMP and blaVIM genes. Twelve (1.5.%) A. baumannii isolates were recovered from clinical infections. Five (41.6%) of isolates were found to be imipenem and meropenem resistant (MIC ranged 128-256 μg mLG 1 ), of which, 4 (66.6%) gave positive result with the imipenem-EDTA disk and modified Hodg test. PCR experiments showed only two (33.3%) isolates were harbored blaOXA-23 gene. The present findings suggest that emergence of OXA-23 carbapenemase-producing A. baumannii clinical isolates in Najaf hospitals.

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“…Besides resistance genes, resistance plasmids can carry other genes that also contribute to the pathogenicity of microorganisms (4). The integrons can house several resistance genes in Gramnegative bacteria.…”

mentioning

confidence: 99%

Detección y caracterización microbiológica de enterobacterias multidrogorresistentes transportadoras del gen modificador de aminoglicósidos durante tres décadas en un hospital universitario de Río de Janeiro

et al. 2014

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Introduction: Multidrug-resistant Enterobacteriaceae, particularly those resistant to gentamicin, have become one of the most important causes of nosocomial infections. Objective: We sought to investigate the presence of genes conferring resistance to aminoglycosides, specially to gentamicin, in Klebsiella pneumoniae and Escherichia coli multidrug-resistant strains isolated from different clinical materials among patients hospitalized in a university hospital in Rio de Janeiro, Brazil. Materials and methods:Ten colonization strains and 20 infection strains were evaluated during three decades (1980 to 2010) using selective media containing 8 µg/ml of gentamicin. Thirty strains were tested for antimicrobial susceptibility. Twenty two strains were subjected to plasmid DNA extraction and 12 to hybridization assays using as probe a 1.9 kb plasmid DNA fragment from one of the K. pneumoniae strains isolated from faecal samples. This fragment was sequenced and assigned to the GQ422439 GenBank record. PCR was also performed using oligonucleotides designed for aminoglycosidemodifying enzymes. Results: An accC2 acetylase, besides transposons and insertion sequences, were evidenced. Twentyfour (80%) of the isolates were positive for the aacC2 gene in agreement with antibiotic susceptibility testing profiles, indicating the persistent presence of this gene throughout the three decades. We detected high molecular weight plasmids in 54,5% of the strains. Of the tested strains, 91% showed positive signal in the hybridization assays. Conclusion: A gene codifying for one specific aminoglycoside-modifying enzyme was detected all throughout the three decades. Our data back the adoption of preventive measures, such as a more conscious use of antimicrobial agents in hospital environments, which can contribute to control the dissemination of microorganisms harboring resistance gene plasmids.

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Resistance Plasmid Families in Enterobacteriaceae

Cited by 1,068 publications

References 122 publications

Molecular characterization of extended-spectrum β-lactamase-producing Shigella isolates from humans in South Africa, 2003–2009

Molecular characterization of extended-spectrum β-lactamase-producing Shigella isolates from humans in South Africa, 2003–2009

First Report of the blaOXA-23 Gene in a Clinical Isolates of Acinetobacter baumannii in Najaf Hospitals-Iraq

Detección y caracterización microbiológica de enterobacterias multidrogorresistentes transportadoras del gen modificador de aminoglicósidos durante tres décadas en un hospital universitario de Río de Janeiro

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