First Report of the blaOXA-23 Gene in a Clinical Isolates of Acinetobacter baumannii in Najaf Hospitals-Iraq

Abstract

Carbapenemase producing Acinetobacter baumannii is frequently associated with nosocomial infections. Increasing resistance to carbapenems, may significantly reduce the choice of effective antibiotics. This study was conducted to determine the occurrence of carbapenemase producing A. baumannii isolates obtained from Najaf hospitals. Isolates were identified according to API 20NE system and more confirmed using 16Sr RNA gene. Carbapenem susceptibility was assayed by microbroth dilution and other antibiotics using disks diffusion test. Phenotypic detection of carbapenemase was performed using the imipenem-EDTA disk and modified Hodg tests. Then isolates were subjected to monoplex PCR targeting blaOXA-23, blaIMP and blaVIM genes. Twelve (1.5.%) A. baumannii isolates were recovered from clinical infections. Five (41.6%) of isolates were found to be imipenem and meropenem resistant (MIC ranged 128-256 μg mLG 1 ), of which, 4 (66.6%) gave positive result with the imipenem-EDTA disk and modified Hodg test. PCR experiments showed only two (33.3%) isolates were harbored blaOXA-23 gene. The present findings suggest that emergence of OXA-23 carbapenemase-producing A. baumannii clinical isolates in Najaf hospitals.

Unmasking the Resistance: Detecting Carbapenem Genes in Acinetobacter baumannii Isolated from some Hospitals in Najaf and Baghdad

“…Our findings are in contrast to the results reported by [22], who reported a sensitivity of 60% for the MHT. Similarly, [31,32]reported sensitivities of 33% and 20%, respectively, which are more consistent with our study's findings.…”

“…In terms of carbapenemase-encoding genes, bla VIM was detected in 18% of the isolates, resembling the findings of [32] but differing significantly from the studies conducted by [33][34][35]. Interestingly, bla SPM was found in 50% of the isolates, contradicting the findings of [34], where none of the tested isolates carried this gene.…”

Frist Detection of The blaOXA-23 Genes in Clinical Isolates of Acinetobacterbaumanniiin Babylon Hospitals-Iraq

“…2) . In the study in Iraq , Alsehlawi, andhiscolleagues who found 2(40%) A. baumannii isolates had blaOXA-23 genes positive in Najaf hospitals (18) . In contrast in Taiwan study , Lee, and hiscolleagues documented only one isolate (4%) of A. baumannii was harbored blaOXA-23 gene (25).…”

“…Out of the 10 A. baumannii isolates which were enrolled in this study, 6 (60%) isolates were found to produce the carbapenemase enzyme by MHT and all the remaining isolates were found to be carbapenemase negative . In a previous local study, Alsehlawi and hiscolleagues reported that 4 (33.3%) of A. baumannii isolates recovered from Najaf hospitals were confirmed as carbapenemase producer using modified Hodge's test, whereas the same isolate gave negative result with imipenem-EDTA synergy test (18). Another study from Croatia the Hodge test showed that 74% (72/97) of the A. baumannii isolates were positive for carbapenemase production (19) , whereas in a study from Pakistan has shown that 17 % of A. baumannii were positive for carbapenemase production by MHT (20).…”

“…The present study showed that (10%) of the isolates gave positive results by imipenem-EDTA disk test . Different studies which have used the IMP-EDTA to detect MBLs production in A. baumannii reported that (33%) of isolates have enhancement of inhibition zone, with the IMP-EDTA test (18) . However, there are four isolates which gave negative results with EDTA disk synergy test.…”