Resistance of wheat cultivars and breeding lines to septoria tritici blotch caused by isolates of <i>Mycosphaerella graminicola</i> in field trials

Brown, J. K. M.; Kema, G.H.J.; Forrer, Hans‐Rudolf; Verstappen, E.C.P.; Arraiano, L. S.; Brading, P. A.; Foster, E. M.; Fried, P. M.; Jenny, Eveline

doi:10.1046/j.1365-3059.2001.00565.x

Cited by 66 publications

(105 citation statements)

References 28 publications

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“…From the host's standpoint (cultivar), the interaction with certain isolates in a given geographical area allowed the manifestation of resistance genes specific for those isolates, which were perpetuated through the process of artificial selection aimed at the formation of new cultivars. In agreement with these results, evidences of differential interactions between Septoria tritici isolates and wheat cultivars have been found in Israel, Morocco, United States and England, suggesting that the physiological specialization of the pathogen has occurred (Eyal et al, 1973;King et al, 1983;Saadaoui, 1987;Ahmed et al, 1995;Kema et al, 1996aKema et al, , 1996bBrown et al, 2001).…”

Section: Results and Discussion Experiments 1: Castelar-argentinasupporting

confidence: 59%

“…The inoculation was made 70 days after plant emergence (DAE), corresponding to the flag leaf phenological stage. The subplots of each cultivar were inoculated with a spore suspension of the corresponding Septoria tritici isolate, using the leaf sprinkling inoculation technique (Dhingra & Sinclair, 1986;Eyal et al, 1987). The inoculum concentration was10 6 -10 8 spores mL -1 .…”

Section: Evaluation Of Resistancementioning

confidence: 99%

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Aggressiveness and physiological specialization of Septoria tritici Rob. isolates

Gieco¹,

Dubcovsky²,

Camargo³

2004

Sci. agric. (Piracicaba, Braz.)

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Pathogenicity tests verifying the behavior of Septoria tritici isolates should be considered as a priority in the selection of resistant wheat materials to this pathogen, since the aggressiveness of each isolate can vary significantly, causing problems in the evaluation and selection of resistant genotypes. The objective of this work was to determine whether physiologic specialization exists among Argentinean and American Septoria tritici isolates, through the analysis of their pathogenicity on cultivars and lines of bread wheat (Triticum aestivum L.). The experiments were carried out in Castelar-Argentina and in Davis-USA. In Castelar, a split plot design (n = 4) was used. The cultivars or lines were randomized in the plots and the isolates in the subplots. Each subplot consisted of three plants belonging to a cultivar or line. In Davis, a strip split plot design (n = 6) was used. A pot containing three plants of each cultivar or line constituted the experimental plots. In both sites, the inoculation was made at the flag leaf phenological stage through foliar aspersion of a conidial suspension adjusted to 10 6 -10 8 conidia mL -1. Evaluations were made by recording the leaf area covered with pycnidia (LACP) at the flag leaf stage with the use of rating scales. Differences (P ≤ 0.0001) in LACP were detected among cultivars or lines. Isolate effects and the interaction cultivar × isolate were significant (P ≤ 0.0001). Variations in aggressiveness and virulence were found among the isolates, indicating the presence of horizontal and vertical resistance in the host cultivars. Cada subparcela foi composta por três plantas pertencentes a um determinado cultivar. Na segunda localidade, foi adotado um delineamento em blocos casualizados e arranjo em faixas, com seis repetições, onde as parcelas experimentais foram constituídas por um vaso contendo três plantas de cada cultivar. A inoculação foi feita no estádio fenológico de folha bandeira em ambas localidades com uma suspensão de conídios ajustada a uma concentração de 10 6 -10 8 esporos mL -1 , empregando a técnica de inoculação por aspersão foliar. As avaliações foram feitas registrando-se a área foliar coberta por picnídios (AFCP) no estádio de folha bandeira com auxílio de escalas de notas. Em ambas localidades foram detectadas diferenças significativas (P ≤ 0,0001) em AFCP entre cultivares. Foram também detectados efeitos significativos de isolados e da interação cultivares x isolados (P ≤ 0,0001). Foram detectadas variações na agressividade e virulência dos isolados sugerindo a presença de resistência horizontal e vertical no material vegetal testado. Testes de patogenicidade destinados a verificar o comportamento de isolados de Septoria tritici devem ser considerados como uma prioridade na seleção de materiais de trigo resistentes a este patógeno. Palavras-chave: Mycosphaerella graminicola, Triticum aestivum, mancha foliar, resistência vertical, resistência horizontal Aggressiveness and physiological specialization of S. tritici isolates 415

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Section: Results and Discussion Experiments 1: Castelar-argentinasupporting

confidence: 59%

Section: Evaluation Of Resistancementioning

confidence: 99%

Aggressiveness and physiological specialization of Septoria tritici Rob. isolates

Gieco¹,

Dubcovsky²,

Camargo³

2004

Sci. agric. (Piracicaba, Braz.)

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“…Combinations of genotypes and isolates were not replicated equally, so the median polish residual for each genotype-isolate pair was multiplied by the square root of the number of plantlets scored, to ensure that all residuals, with the exception of outliers, were sampled from a single normal distribution (Chartrain et al ., 2004). The statistical significance of the deviation of the median polish residual from a normal distribution was tested by Grubbs' method (Sokal & Rohlf, 1981;Brown et al ., 2001). This part of the analysis was completed using Microsoft excel 2002.…”

Section: Discussionmentioning

confidence: 99%

Genotype‐isolate interaction for resistance to black stem in sunflower (Helianthus annuus)

et al. 2007

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Two experiments were undertaken to determine the partial resistance of sunflower genotypes to seven isolates of Phoma macdonaldii . In the first experiment, 28 genotypes, including recombinant inbred lines and their parents, M6 mutant lines developed by gamma irradiation, and some genotypes from different geographical origins, were used. The experiment consisted of a split-plot design with three replications, each with 12 seedlings per genotype per isolate, in controlled conditions. Seven days after inoculation, plantlets were scored on a 1-9 scale for percentage necrotic area. Highly significant differences were observed among genotypes, isolates and their interactions. The presence of a differential interaction between genotypes and P. macdonaldii isolates was confirmed in a second experiment using 12 genotypes representing large variability for partial resistance to P. macdonaldii isolates, as identified in the first experiment. Inbred lines B454/03, ENSAT-B5 and LC1064C were the most susceptible sunflower genotypes, whereas two American lines SDR19 and SDR18 presented high partial resistance to all P. macdonaldii isolates studied. The least and most aggressive isolates were MA6 and MP3, respectively. Isolates interacted differentially with sunflower genotypes. This study identified two genotypes (AS613 and PAC2) presenting specific resistance to isolate MP8. The results also showed a wide range of isolate-nonspecific partial resistances among the lines tested. The information presented here could assist sunflower breeders to choose parents of crosses for breeding of durable resistance to phoma black stem disease.

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“…Pycnidial coverage on leaf surface was assessed as a measure of the level of infection and/or resistance. Although other scoring methods have been proposed for assessing the disease severity of STB in wheat (McCartney et al 2002;Adhikari et al 2003), the scoring of disease severity based on a visual estimation of the percent leaf area covered by lesions bearing pycnidia still remains as the most common approach (Kema et al 1996a;Brown et al 2001;Chartrain et al 2004). Pycnidial coverage is considered to be more accurate because symptoms cannot be confounded with natural senescence or other pathogens.…”

Section: Discussionmentioning

confidence: 99%

New sources of resistance to Septoria tritici blotch in wheat seedlings

2016

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The resistance of a set of Argentine wheat cultivars, differentials, and foreign lines with known genes of resistance to Zymoseptoria tritici was assessed at the seedling stage against a set of molecularly characterized Argentine fungal isolates under three experimental conditions. The experimental design was a split-splitplot with two replications. The environment conditions were the main plots, the fungal isolates were the subplots and the wheat lines with known resistance genes or used as differential lines or the Argentine commercial cultivars were the sub-subplots. Sixteen fungal isolates were inoculated on 23 wheat accessions including 12 foreign lines with known resistance genes, two differential lines, and nine Argentine cultivars that were selected on the basis of their moderate resistance to natural infection, to identify isolate-specific and partial resistances. The genotypes TE 9111 and Oasis among the foreign lines along with the Argentine cultivars Pro INTA Puntal, Klein Volcán and Buck 75 Aniversario expressed the highest number of specific-resistance interactions. Flame, Tadinia and Kavkaz among the foreign lines showed partial resistances to a higher number of fungal isolates; a comparable resistance pattern was also obtained in the Argentine cultivars Klein Sagitario, Klein Dragón, Don Ernesto and Buck Arriero. Our study demonstrated that the presence of resistance genes in several foreign lines against Septoria tritici blotch is effective against some of the Argentine fungal isolates. In addition, the Argentinean wheat cultivars that showed isolate-specific and nonspecific resistances probably carry novel resistance genes against the pathogen.

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Resistance of wheat cultivars and breeding lines to septoria tritici blotch caused by isolates of Mycosphaerella graminicola in field trials

Cited by 66 publications

References 28 publications

Aggressiveness and physiological specialization of Septoria tritici Rob. isolates

Aggressiveness and physiological specialization of Septoria tritici Rob. isolates

Genotype‐isolate interaction for resistance to black stem in sunflower (Helianthus annuus)

New sources of resistance to Septoria tritici blotch in wheat seedlings

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