Resistance of three wild tuber‐bearing potatoes to the Colorado potato beetle

Abstract

The resistance of Solanum okadae Hawkes & Hjert. (PI 458367), Solanum oplocense Hawkes (PI 473368), and Solanum tarijense Hawkes (PI 414150) to the Colorado potato beetle, Leptinotarsa decemlineata (Say) (Chrysomelidae: Chrysomelini), was studied. In replicated field trials all three accessions showed a high level of resistance to the beetle. No significant genetic variability between genotypes of the same species was found. Results from host acceptance behavior experiments, suitability for larval development tests, foliage consumption tests, and adult survival and oviposition tests supported the hypothesis that the mode of resistance differs between the three wild Solanum species. Solanum okadae and S. oplocense affected host acceptance and consumption. Because the beetle reacted differently to these two species it was hypothesized that the antifeedant chemical(s) differed in nature or quantity. S. tarijense contrasted with the other two species by affecting mostly adult colonization and oviposition.

Comparative Metabolite Profiling ofSolanum tuberosumagainst Six WildSolanumSpecies with Colorado Potato Beetle Resistance

“…The wild species showed low levels of defoliation in the field without imidacloprid (Figure 1c−h), which concurs with previous observations. 17,18,23,24 An objective of the study was to find metabolites that can be used as markers for screening CPB resistance in potato germplasm grown in greenhouses. Therefore, comparative analysis of foliar metabolites in S. tuberosum and the wild species was done with greenhouse-grown plants.…”

Cytological mechanisms of 2n pollen formation in the wild potato Solanum okadae and pollen-pistil relations with the cultivated potato, Solanum tuberosum

“…okadae Hawkes et Hjert (oka, 2n = 2x = 24) is a wild species endemic to Argentina and Bolivia (Hawkes and Hjerting 1983;Ochoa 1990;Spooner and Salas 2006). No genetic studies have been performed with this species and, although it is of potential value in breeding (Hanneman 1996;Pelletier et al 2001;USDA 2002), its crossability relations with tbr are unknown. Furthermore, accessions from the two countries of origin available at the Potato Germplasm Bank at Balcarce, Argentina, have different morphological phenotypes (Clausen and Ispizúa 2005).…”

Complete 1H and 13C NMR spectral assignments for the glycoalkaloid dehydrocommersonine

“…After extraction and clean‐up, foliage from S. oplocense (PI 473368) afforded predominantly one glycoalkaloid fraction and a crystalline portion appeared homogeneous on HPLC. A positive HR‐ESI‐MS spectrum of the sample gave an [M + H] + ion at m/z 1046.5499 (C 51 H 84 NO 21 requires 1046.5536) consistent with a dehydrocommersonine moiety .…”

“…). It was first isolated from root tissue initiated from a callus culture of S. chacoense but its presence has since been confirmed in the foliage of several other wild Solanum species including S. oplocense . As part of a research program involving studies of wild Solanum species suitable for potato breeding, we wish to report the first complete 1 H and 13 C NMR spectral analysis of the glycoalkaloid dehydrocommersonine.…”