Comparative Metabolite Profiling ofSolanum tuberosumagainst Six WildSolanumSpecies with Colorado Potato Beetle Resistance

Abstract

The Colorado potato beetle Leptinotarsa decemlineata (Say) (CPB) is a coleopteran herbivore that feeds on the foliage on Solanum species, in particular, potato. Six resistant wild Solanum species were identified, and two of these species had low levels of glycoalkaloids. Comparative analysis of the untargeted metabolite profiles of the foliage using UPLC-qTOF-MS was done to find metabolites shared between the wild species but not with Solanum tuberosum (L.) to identify resistance-related metabolites. It was found that only S. tuberosum produced the triose glycoalkaloids solanine and chaconine. Instead, the six wild species produced glycoalkaloids that shared in common tetrose sugar side chains. Additionally, there were non-glycoalkaloid metabolites associated with resistance including hydroxycoumarin and a phenylpropanoid, which were produced in all wild species but not in S. tuberosum.

Mismatch repair deficiency increases the transfer of antibiosis and antixenosis properties against Colorado potato beetle in somatic hybrids ofSolanum tuberosum+S. chacoense

“…Foliar leptine glycoalkaloids are associated with poor larval development and high larval mortality. 10,17,34 These observations were also confirmed by our results. CPB larvae fed on S. chacoense HL leaves, which are known to produce the largest amount of leptine glycoalkaloids, developed slowly, and none reached the adult stage.…”

“…15,16 In experiments conducted by Lorenzen et al, 10 CPB larvae fed with S. chacoense leaves developed more slowly than those fed cultivated potato leaves. Similar results were obtained by Tai et al, 17 who found that the foliar leptine glycoalkaloids are associated with a decrease in adult beetle feeding and an increase in larvae mortality.…”

“…On resistant SHs, less than 30% of the larvae survived for 23 days. Larvae fed on resistant plants required [13][14][15][16][17][18][19] days to reach L4 instar. The LDIs indicate similar results: larvae fed on resistant hybrids had a far lower index (LDI between 0 and 30) than those fed on susceptible clones (SHs and BCs) or cultivated potato.…”

Metabolite profiles of maize leaves in drought, heat and combined stress field trials reveal the relationship between metabolism and grain yield

“…Moreover, metabolic markers started to draw attention due to their close relationship with yield phenotypes (Fernie and Schauer, 2009;Redestig et al, 2011;Riedelsheimer et al, 2012aRiedelsheimer et al, , 2012bWitt et al, 2012;Degenkolbe et al, 2013). The accumulation of some metabolites has been reported to be directly related to the performance of potato (Solanum tuberosum) cultivars in beetle resistance in the field (Tai et al, 2014). Additionally, identical genomic regions were mapped as both agronomic and metabolic quantitative trait loci in field-grown maize and wheat (Triticum aestivum), indicating the utility of metabolic traits for breeding selection (Riedelsheimer et al, 2012b;Hill et al, 2015).…”

Estudo químico de extratos de plantas da família Solanaceae com atividade a fungos fitopatogênicos

“…A atividade antifúngica já foi descrita para α-solamargina, a qual inibiu o desenvolvimento micelial dos fungos P. medicaginis e R. solani WEISSENBERG, 1994). A bioatividade da leptinina I está relacionada com a inibição da herbívoria causada pelo besouro-da-batata (Leptinotarsa decemlineata) o qual é responsável por perdas entre 30-50% em plantações de batata (SHAKYA;NAVARRE, 2008;TAI et al, 2014). O que demonstra que os compostos α-solamargina, β-solamarina e leptinina I, "supostamente" avaliados neste trabalho, apresentam as mais diversas bioatividades.…”