Resistance of Neochloris oleoabundans to six terpenes applicable as green contamination control agents

Molina-Aulestia, Denisse Tatiana; Soccol, Carlos Ricardo; Júnior, Antônio Irineudo Magalhães; González-Marín, Oranys Isaac; Souza, Caroline Gusmão de; Medeiros, Adriane Bianchi Pedroni; Klein, Eduardo; Carvalho, Júlio César de

doi:10.1007/s10811-021-02612-y

Cited by 4 publications

(1 citation statement)

References 48 publications

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“…Though, there are different types of bacteria in the natural microalgae phycosphere (the environment surrounding microalgae); bacterial cell concentration is often determined as "total bacteria," without distinction of species and genera and can reach cell counts between 1x10 6 to 1x10 9 cells.mL − 1 (Fulbright et al 2018; Di Caprio 2020). In previous studies, the toxicity of six terpenes (eugenol, linalool, limonene, alpha, and beta-pinene, and trans-cinnamaldehyde) on the microalga N. oleoabundans UTEX 1185 were evaluated (Molina-Aulestia et al 2021). Currently, techniques such as MIC (minimal inhibitory concentration) make it possible to determine the minimum concentration at which a substance inhibits the growth of microorganisms (Moreira et al 2005;Parvekar et al 2020).…”

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confidence: 99%

Fine Resolution Analysis of bacterial communities associated with Neochloris oleoabundans cultures, and insights into terpenes as contamination control agents

Molina-Aulestia

Carvalho

Pereira

et al. 2022

Preprint

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Biological contamination is one of the main bottlenecks in the production of microalgae, reducing quality and productivity, sometimes leading to complete loss of the cultures. This work evaluated the presence of bacterial contaminants in N. oleoabundans cultures through HTS and 16S analysis and their susceptibility to 6 natural terpenes (α-pinene, β-pinene, limonene, trans-cinnamaldehyde, linalool, and eugenol). The principal phyla identified were Proteobacteria, Bacteroidetes and Actinobacteria, and based on these data, 89 bacterial isolates of seven genera were obtained (36 Aureimonas sp., 27 Microbacterium sp., 5 Pseudomonas sp., 9 Bacillus sp., 14 Shinella sp., 1 Brevundimonas sp., and 1 Exiguobacterium sp.). The minimal inhibitory concentration of terpenes was 6.25 mg. mL− 1, with a reduction in bacterial counts of most isolates by 79% − 99%. The use of selected terpenes can be a pathway towards eco-friendly contamination control in microalgae cultures.

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Section: Introductionmentioning

confidence: 99%

Fine Resolution Analysis of bacterial communities associated with Neochloris oleoabundans cultures, and insights into terpenes as contamination control agents

Molina-Aulestia

Carvalho

Pereira

et al. 2022

Preprint

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Fine resolution analysis of bacterial communities associated with Neochloris oleoabundans culture and insights into terpenes as contamination control agents

Molina-Aulestia

Carvalho

Pereira

et al. 2023

World J Microbiol Biotechnol

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Biological contamination is one of the main bottlenecks in microalgae production, reducing quality and productivity and sometimes leading to the complete loss of the cultures. Selecting terpenes can be a pathway toward eco-friendly contamination control in microalgae cultures. This work evaluated the presence of bacterial contaminants in N. oleoabundans cultures through HTS and 16S analysis and their susceptibility to six natural terpenes (α-pinene, β-pinene, limonene, trans-cinnamaldehyde, linalool, and eugenol). The principal phyla identi ed were Proteobacteria, Bacteroidetes, and Actinobacteria, and based on these data, 89 bacterial isolates of seven genera were obtained (36 Aureimonas sp., 27 Microbacterium sp., 5 Pseudomonas sp., 9 Bacillus sp., 14 Shinella sp., 1 Brevundimonas sp., and 1 Exiguobacterium sp.). The minimal inhibitory concentration of terpenes was 6.25 mg L -1 , with a reduction in bacterial counts of most isolates by 79% -99%.

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A review on design-build-test-learn cycle to potentiate progress in isoprenoid engineering of photosynthetic microalgae

Lan

et al. 2022

Bioresource Technology

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Resistance of Neochloris oleoabundans to six terpenes applicable as green contamination control agents

Cited by 4 publications

References 48 publications

Fine Resolution Analysis of bacterial communities associated with Neochloris oleoabundans cultures, and insights into terpenes as contamination control agents

Fine Resolution Analysis of bacterial communities associated with Neochloris oleoabundans cultures, and insights into terpenes as contamination control agents

Fine resolution analysis of bacterial communities associated with Neochloris oleoabundans culture and insights into terpenes as contamination control agents

A review on design-build-test-learn cycle to potentiate progress in isoprenoid engineering of photosynthetic microalgae

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