Resistance of Multipotent Mesenchymal Stromal Cells to Anoxia In Vitro

Buravkova, Ludmila; Андреева, Е.Р.; Rylova, J. V.; Grigoriev, A. I.

doi:10.5772/28433

Cited by 3 publications

(1 citation statement)

References 80 publications

(79 reference statements)

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“…The stem cell niche concept was proposed by Schofield in 1978 [42], and several researchers have tried to elucidate the confusion and controversy over it [43–45]. “Stem cell niche” can be defined as the anatomical compartment composed of cellular and acellular components that orchestrate both systemic and local signals to control the rate of stem cell proliferation, to determine the fate of stem cell daughters, and to protect stem cells from exhaustion or death [46–48]. The cellular and acellular components of the stem cell niche can be divided into four main groups of key factors, namely, the regulatory molecules (O 2 , nutrients, and cytokines), other cells (3D context, cell-cell contacts, autocrine, and paracrine signals), extracellular matrix (immobilized and released factors, structure, topology, and stiffness), and physical factors (flow shear, compression, stretch, and electrical signals) [41].…”

Section: Stem Cell Nichementioning

confidence: 99%

Hypoxic Culture Conditions as a Solution for Mesenchymal Stem Cell Based Regenerative Therapy

Haque

Rahman

Kasim

et al. 2013

The Scientific World Journal

184

156

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Cell-based regenerative therapies, based on in vitro propagation of stem cells, offer tremendous hope to many individuals suffering from degenerative diseases that were previously deemed untreatable. Due to the self-renewal capacity, multilineage potential, and immunosuppressive property, mesenchymal stem cells (MSCs) are considered as an attractive source of stem cells for regenerative therapies. However, poor growth kinetics, early senescence, and genetic instability during in vitro expansion and poor engraftment after transplantation are considered to be among the major disadvantages of MSC-based regenerative therapies. A number of complex inter- and intracellular interactive signaling systems control growth, multiplication, and differentiation of MSCs in their niche. Common laboratory conditions for stem cell culture involve ambient O2 concentration (20%) in contrast to their niche where they usually reside in 2–9% O2. Notably, O2 plays an important role in maintaining stem cell fate in terms of proliferation and differentiation, by regulating hypoxia-inducible factor-1 (HIF-1) mediated expression of different genes. This paper aims to describe and compare the role of normoxia (20% O2) and hypoxia (2–9% O2) on the biology of MSCs. Finally it is concluded that a hypoxic environment can greatly improve growth kinetics, genetic stability, and expression of chemokine receptors during in vitro expansion and eventually can increase efficiency of MSC-based regenerative therapies.

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Section: Stem Cell Nichementioning

confidence: 99%

Hypoxic Culture Conditions as a Solution for Mesenchymal Stem Cell Based Regenerative Therapy

Haque

Rahman

Kasim

et al. 2013

The Scientific World Journal

184

156

View full text Add to dashboard Cite

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Tissue-Specific Peculiarities of Vibration-Induced Hypoxia in Rabbit Liver and Kidney

Vorobieva

Шабанов

2019

Bull Exp Biol Med

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Insight in Hypoxia-Mimetic Agents as Potential Tools for Mesenchymal Stem Cell Priming in Regenerative Medicine

Nowak-Stępniowska

Osuchowska

Fiedorowicz

et al. 2022

Stem Cells International

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Hypoxia-mimetic agents are new potential tools in MSC priming instead of hypoxia incubators or chambers. Several pharmaceutical/chemical hypoxia-mimetic agents can be used to induce hypoxia in the tissues: deferoxamine (DFO), dimethyloxaloylglycine (DMOG), 2,4-dinitrophenol (DNP), cobalt chloride (CoCl2), and isoflurane (ISO). Hypoxia-mimetic agents can increase cell proliferation, preserve or enhance differentiation potential, increase migration potential, and induce neovascularization in a concentration- and stem cell source-dependent manner. Moreover, hypoxia-mimetic agents may increase HIF-1α, changing the metabolism and enhancing glycolysis like hypoxia. So, there is clear evidence that treatment with hypoxia-mimetic agents is beneficial in regenerative medicine, preserving stem cell capacities. These agents are not studied so wildly as hypoxia but, considering the low cost and ease of use, are believed to find application as pretreatment of many diseases such as ischemic heart disease and myocardial fibrosis and promote cardiac and cartilage regeneration. The knowledge of MSC priming is critical in evaluating safety procedures and use in clinics. In this review, similarities and differences between hypoxia and hypoxia-mimetic agents in terms of their therapeutic efficiency are considered in detail. The advantages, challenges, and future perspectives in MSC priming with hypoxia mimetic agents are also discussed.

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Resistance of Multipotent Mesenchymal Stromal Cells to Anoxia In Vitro

Cited by 3 publications

References 80 publications

Hypoxic Culture Conditions as a Solution for Mesenchymal Stem Cell Based Regenerative Therapy

Hypoxic Culture Conditions as a Solution for Mesenchymal Stem Cell Based Regenerative Therapy

Tissue-Specific Peculiarities of Vibration-Induced Hypoxia in Rabbit Liver and Kidney

Insight in Hypoxia-Mimetic Agents as Potential Tools for Mesenchymal Stem Cell Priming in Regenerative Medicine

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