Resistance of early‐maturing European maize germplasm to sugarcane mosaic virus (SCMV) and maize dwarf mosaic virus (MDMV)

Abstract

Sugarcane mosaic virus (SCMV) and maize dwarf mosaic virus (MDMV) are the most important viruses of maize in Europe. In field and greenhouse experiments, 122 early-maturing European maize inbreds (45 fiint and 77 dent lines) were evaluated for their reaction to artificial inoculation by SCMV and MDMV. Three dent inbreds (D21, D32, FAP1360 A) with complete resistance and four dent inbreds (D06, D09, R2306, FAP1396A) with partial resistance against both potyviruses under both greenhouse and field conditions were identified. All other inbreds were highly susceptible to both SCMV and MDMV. Selection for virus resistance in maize breeding could be performed with only one virus at a time because all inbreds resistant to SCMV were also resistant to MDMV. Rank correlations between percentages of infected plants in greenhouse and field trials ranged from 0.51 to 0.72 for both SCMV and MDMV, suggesting that prescreening of breeding materials for virus resistance can be performed in the greenhouse but final evaluation in multilocation trials in the field is recommended.

Saturation of two chromosome regions conferring resistance to SCMV with SSR and AFLP markers by targeted BSA

“…The early maturing European maize inbreds, FAP1360A, resistant to SCMV, and F7, highly susceptible to SCMV (Kuntze et al 1997), were crossed to produce the F1 generation and were then backcrossed nine times to F7 with two generations per year. SCMV evaluation was performed during the summer seasons at Hohenheim in 1995Hohenheim in , 1996Hohenheim in , 1997Hohenheim in , 1998Hohenheim in and 1999 in the generations BC1 (backcross 1), BC3, BC5, BC7 and BC9, respectively.…”

“…Since the use of insecticides for control of the aphid vectors is ineffective, cultivation of resistant maize varieties is the only way to control SCMV. Kuntze et al (1997) identified three dent inbreds (D21, D32 and FAP1360A) displaying complete resistance to SCMV and maize dwarf mosaic virus (MDMV) under both field and greenhouse conditions in early maturing germplasm. Segregation and QTL analyses uncovered two genomic regions on chromosomes 6S and 3L with major effects on SCMV resistance in two independent populations.…”

Development of RGA-CAPS markers and genetic mapping of candidate genes for sugarcane mosaic virus resistance in maize

“…Owing to the non-persistent transmission, control of aphid vectors by chemical means is not effective and, therefore, cultivation of resistant maize varieties is the most efficient method of virus control. In a study with 122 early maturing European maize inbreds, three lines (FAP1360A, D21 and D32) displayed complete resistance and four lines displayed partial resistance (FAP1396A, D06, D09 and R2306) against SCMV and maize dwarf mosaic virus (MDMV) (Kuntze et al 1997). In field trials, resistance of all three European lines D21, D32 and FAP1360A seemed to be controlled by one to three genes (Melchinger et al 1998).…”

Conversion of AFLP fragments tightly linked to SCMV resistance genes Scmv1 and Scmv2 into simple PCR-based markers

“…Therefore, cultivation of resistant varieties is the most promising approach for controlling of SCMV. Kuntze et al (1997) screened 122 early-maturing European inbred lines for resistance to SCMV and MDMV (maize dwarf mosaic virus) and identified three dent inbreds (D21, D32 and FAP1360A) displaying complete resistance under both field and greenhouse conditions. Two major genes, Scmv1 and Scmv2 (previously named Scm1 and Scm2), conferring resistance to SCMV were mapped to chromosome arms 6S and 3L, respectively, in cross D145 × D32 by quantitative trait loci (QTL) analysis and in cross F7 × FAP1360A by bulked segregant analysis (BSA) ) and QTL analysis (Dussle et al 2000).…”