Resistance Induction and Direct Antifungal Activity of Some Monoterpenes against Rhizoctonia solani, the Causal of Root Rot in Common Bean

Abstract

This study was conducted to evaluate eco-friendly control agents (carvone, cuminaldehyde, and linalool) against Rhizoctonia solani, which causes root rot disease either by induction of defense response or direct antifungal activity. The induction of resistance was examined by detecting the transcription of defense genes and the effect of the tested control agents on the growth and the yield of common bean plants. The growth of R. solani was significantly inhibited after treatment with the tested compounds compared to the untreated control under laboratory conditions. The disease severity of root rot was decreased in common bean plants treated with the tested compounds compared to untreated control plants under greenhouse conditions. Common bean plants treated with the tested control agents expressed defense genes (Phenylalanine ammonia lyase and β-1,3-Glucanase) involved in jasmonic acid (JA) and salicylic acid (SA) signaling pathways with 2–5 fold higher than the control. Treatment of common beans with the tested control agents and fungicide significantly improved the growth and yield characteristics of common bean. Therefore, the use of monoterpenes could be a novel strategy to control this pathogen and consider the first report.

Swietenia mahagoni Leaves Extract: Antifungal, Insecticidal, and Phytochemical Analysis

“…Meanwhile, Tralamazza et al [15] have reported that this species complex is associated with panicle infection in wild rice in Brazil. Numerous research studies have explored the effectiveness of different extracts against R. solani, revealing encouraging outcomes regarding their antifungal properties [16][17][18]. Antifungal properties towards the fungi Aspergillus flavus, Candida spp., and A. niger were detected using water, alcohol, ether, and chloroform-derived extracts of S. macrophylla leaves [19].…”

Photosynthesis, Salicylic Acid Content and Enzyme Activity of Triticum aestivum L. Influenced by the Use of a Seaweed Biostimulant Based on Solieria chordalis

“…Compared to the untreated control, the use of fungicide alone or in combination with the RSB induced a slight, but not signi cant, reduction in the activity of PAL. When testing the effects of monoterpenes and the fungicide based on toclophos-methyl + thiram on common bean, Derbalah et al (2022) reported no signi cant differences in the expression of PAL when the fungicide was applied to plants (compared to the untreated control). On the other hand, the use of monoterpenes increased the expression of this enzyme, similar to the results observed with the use of the RSB on wheat plants.…”

Morphological, Molecular Identification and Pathogenicity of Rhizoctonia solani Kuhn: A Seed and Soil Borne Pathogen Infecting French Bean

“…The fungus was identified and maintained on PDA medium, with stock cultures being subcultured at intervals of 20-25 days [10]. Pathogen was morphologically identified with micro and macroscopically observed using compound microscope [11].…”

“…Spedaletti et al [7] identified R. solani based on the morphological characteristics viz., mycelium color, sclerotia formation, and nuclei count per hyphal cell. Derbalah et al [11] isolated R. solani from naturally infected common bean plants displaying symptoms of root rot and it was identified based on its morphological and microscopic characteristics. Heflish et al [17] isolated Rhizactonia solani from bean plants exhibiting characteristic symptoms on potato dextrose agar (PDA) plates and further purification of the pathogen was obtained by following hyphal tip technique.…”