Resistance in Phaseolus vulgaris to the Severe Strain of Bean Yellow Mosaic Virus

Provvidenti, R.; Schroeder, W. T.

doi:10.1094/phyto-63-196

Cited by 31 publications

(18 citation statements)

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“…Though both ClYVV and BYMV have been reported from this region in the past (Dickson and Natti, 1968;Provvidenti and Schroeder, 1973) their incidence has increased in frequency as part of an emerging complex of aphid-transmitted viruses (Larsen et al, 2002(Larsen et al, , 2008Nault et al, 2004;Shah et al, 2006;Tolin and Langham, 2010) that have been associated with the accidental introduction of the soybean aphid (Aphis glycines Matsumara) to the United States (Hill et al, 2001;Ragsdale et al, 2004). While it is unknown whether A. glycines can transmit ClYVV, the virus is transmitted in a nonpersistent manner by numerous noncolonizing aphid species that are present in the region (Nault, 1997;Provvidenti and Morales, 2005b;Shah et al, 2006).…”

Section: Resistance To Clover Yellow Vein Virus In Common Bean Germplasmmentioning

confidence: 99%

“…The germplasm was evaluated for resistance to the 'New York' strain of ClYVV (ClYVV-NY), obtained from the Rosario Provvidenti collection at the NYSAES (Provvidenti and Schroeder, 1973), and further characterized by recent research (Hart and Griffiths, 2013;Larsen et al, 2008). The ClYVV-NY strain was employed for the resistance evaluation because in previous research this strain has identified the resistance phenotype conditioned by either the bc-3 or bc-3 2 resistance alleles without exception (Hart and Griffiths, 2013).…”

Section: Allele-specific Genotyping Of Pveif4ementioning

confidence: 99%

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Resistance to Clover yellow vein virus in Common Bean Germplasm

Hart

Griffiths

2014

Crop Science

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Clover yellow vein virus (ClYVV) is a potentially devastating viral pathogen of temperate common bean (Phaseolus vulgaris L.) production. Recent research demonstrated that resistance to ClYVV is conditioned by bc‐3 and bc‐32 and that a nonsynonymous mutation in P. vulgaris eukaryotic translation initiation factor 4E (PveIF4E) is the putative functional determinant of this resistance. The objective of this research was to expand the characterization of ClYVV resistance in common bean through greenhouse‐based phenotypic evaluation of 391 accessions of the USDA P. vulgaris Core Collection, a collection of 99 snap bean cultivars and breeding lines, and an extended host differential panel of 75 genotypes with known response to other viral pathogens and to determine if previously designed Kompetitive Allele Specific Polymerase Chain Reaction (KASP) assays could be validated for future bc‐3 resistance allele mining. As a result, one accession from the Core Collection, one additional snap bean breeding line, and 19 dry bean genotypes representing most major market classes of common bean were identified to be resistant. All genotypes that remained symptomless throughout the evaluation were demonstrated to possess mutations in PveIF4E that corresponded with either bc‐3 or bc‐32. In addition, the bc‐u bc‐22 allele combination and the By‐2 allele were also demonstrated to condition resistance phenotypes to ClYVV. These results synthesize a new model for genetic resistance to ClYVV and validate the use of the KASP assays for large‐scale bc‐3 allele mining.

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Section: Resistance To Clover Yellow Vein Virus In Common Bean Germplasmmentioning

confidence: 99%

Section: Allele-specific Genotyping Of Pveif4ementioning

confidence: 99%

Resistance to Clover yellow vein virus in Common Bean Germplasm

Hart

Griffiths

2014

Crop Science

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“…Resistance to ClYVV was associated with three recessive genes ( cyv , desc , bc ‐ 3 ) initially thought to be independent (Providenti and Schroeder ; Provvidenti ; Sato et al. ; Larsen et al.…”

Section: Introductionmentioning

confidence: 99%

“…Resistance to ClYVV was associated with three recessive genes (cyv, desc, bc-3) initially thought to be independent (Providenti and Schroeder 1973;Provvidenti 1987;Sato et al 2003;Larsen et al 2008), although the effectiveness of bc-3 was not conclusive in some of these studies (Larsen et al 2008). Hart and Griffiths (2013) provided clear evidence that the three genes were a series of recessive alleles at the Bc-3 locus and showed unique potyvirus strain-and species-specific resistance spectra.…”

Section: Introductionmentioning

confidence: 99%

A New Virulent Isolate of Clover Yellow Vein Virus on Phaseolus vulgaris in Bulgaria

Pasev

Kostova

Turina³

2014

Journal of Phytopathology

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Potyviruses are a common threat for snap bean production in Bulgaria. During virus surveys of bean plots in the south central region, we identified an isolate of Clover yellow vein virus (ClYVV), designated ClYVV 11B, by indirect ELISA and RT‐PCR causing severe mosaic symptoms and systemic necrosis. Indirect and direct ELISA using ClYVV antisera differentiated the ClYVV isolate from Bean yellow mosaic virus (BYMV), but serological analysis could not distinguish the Bulgarian isolate ClYVV 11B from an Italian ClYVV isolate used as a reference (ClYVV 505/7). RT‐PCR analyses with specific primers revealed that both isolates were ClYVV. Sequence analysis of an 800 bp fragment corresponding to the coat protein coding region showed 94% identity at the nucleotide level between the two isolates. Phylogenetic analyses of aligned nucleotide sequences available in the database confirmed the existence of two groups of isolates, but ClYVV 11B and ClYVV505/7 belonged to the same group. We compared the virulence of both isolates on a set of differential cultivars and 19 bean breeding lines resistant to Bean common mosaic virus (BCMV) and Bean common mosaic necrosis virus (BCMNV): Bulgarian isolate ClYVV 11B was able to infect systemically all tested bean differential cultivars and breeding lines including those with genotypes Ibc3 and Ibc22; Italian isolate ClYVV 505/7 was not able to infect systemically some differentials with genotypes bc‐ubc1, bc‐ubc22, bc‐ubc2bc3, Ibc12, Ibc22, Ibc3. The role of bc3 gene as a source of resistance to potyviruses is discussed.

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“…However, because reciprocal tests were not made with antisera against BYMV" and PV-2, it may be concluded only that BYMVl possesses an antigenic specificity which is somewhat different from that of the two other strains. Previous studies had shown differences among these three strains in, for example, host range and response to specific resistance genes in Phaseolus vulgaris (Prowidenti & Schroeder, 1973).…”

mentioning

confidence: 94%

Partial purification and serological relationship of three strains of bean yellow mosaic virus

Granett

Prowidenti

1975

Annals of Applied Biology

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S U M M A R YThree biologically distinct strains of bean yellow mosaic virus (BYMVs, BYMVl and PV-2) were partially purified by centrifugation at relatively low g forces. Serologically these strains appeared to be distinct from each other but were related.Our recent isolation of the severe strain of bean yellow mosaic virus (BYMVs) from naturally infected sweet violet (Viola odorata L.) (Prowidenti & Granett, 1974) prompted us to compare serologically this isolate with two other known strains of the virus. Difficulties, however, were encountered in the purification of BYMVS with the method outlined by Damirdagh & Shepherd (1970) and successfully used for an authentic strain of BYMV by Uyemoto, Prowidenti & Schroeder (1972).This paper describes a reliable procedure for the partial purification of BYMVs, BYMVl (pea isolate I ; Hagedorn & Walker, 1950) and PV-2 (pea virus 2; Schroeder & Provvidenti, 1966) and reports their serological comparison in agar gel double diffusion tests.BYMVs and BYMVI were propagated in bean (Phaseolus vukaris L. cv. Red Kidney) and in pea (Pisum sativum L. cv. Ranger) while PV-2 was increased only in pea. Infected bean leaves were harvested 30-40 days after inoculation and pea leaves after 11-21 days. The three strains of BYMV were purified using a modification of the method recently proposed by Huttinga (1973).Each gram of chilled leaves was homogenized in a blender in a cold solution containing 7-5 ml of 0-1 M tris (hydroxy-methyl) aminomethane buffer, z ml of carbon tetrachloride and 2 ml of chloroform. The pH of the extractant was adjusted with thioglycollic acid to 6.5 for BYMVs and 8-5 for BYMVl and PV-2. The homogenate was centrifuged for 10 min at 6000 g (Sorvall RCzB, SS34 rotor, 7000 rev./min) to pellet most plant debris, after which the supernatant fluid was filtered through glass wool. The solution was then centrifuged at 20000g (Spinco Model L, 30 rotor, 15000 rev./min) for I h. The pelleted virus was resuspended in 0 -1 M tris buffer adjusted with HC1 to the same pH as the original extractant. Preparations were further clarified by centrifugation at 3000 g (SS34 rotor, 5000 rev./min) for 5 min. Higher centrifugal forces during either initial clarification or pelleting increased particle aggregation and breakage and reduced virus yield.

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Resistance in Phaseolus vulgaris to the Severe Strain of Bean Yellow Mosaic Virus

Cited by 31 publications

References 0 publications

Resistance to Clover yellow vein virus in Common Bean Germplasm

Resistance to Clover yellow vein virus in Common Bean Germplasm

A New Virulent Isolate of Clover Yellow Vein Virus on Phaseolus vulgaris in Bulgaria

Partial purification and serological relationship of three strains of bean yellow mosaic virus

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