Resistance from agar medium impacts the helical growth of Arabidopsis primary roots

Yan, Jie; Wang, Bochu; Zhou, Yong; Hao, Shilei

doi:10.1016/j.jmbbm.2018.05.018

Cited by 18 publications

(19 citation statements)

References 37 publications

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“…Placing a horizontal obstacle in the way of a vertically growing root reveals a response likely to be induced by a combination of touch sensing and gravity, and resulting in a ‘step‐like’ growth pattern, with only the root tip remaining in contact with the barrier surface (Massa & Gilroy, 2003 ; Lee et al ., 2020 ). Root bending has also been observed in roots grown in a medium consisting of a soft upper layer and a hard lower layer, with roots exhibiting a bending response at the harder layer (Yamamoto et al ., 2008 ; Yan et al ., 2017 , 2018 ). It has been suggested that a zone of ‘mechanical weakness’ is required for the bending process and that this is localized between the growing and mature zones of the root (Bizet et al ., 2016 ).…”

Section: Discussionmentioning

confidence: 99%

Root growth responses to mechanical impedance are regulated by a network of ROS, ethylene and auxin signalling in Arabidopsis

Jacobsen

Jervis

et al. 2021

New Phytologist

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The growth and development of root systems is influenced by mechanical properties of the substrate in which the plants grow. Mechanical impedance, such as by compacted soil, can reduce root elongation and limit crop productivity. To understand better the mechanisms involved in plant root responses to mechanical impedance stress, we investigated changes in the root transcriptome and hormone signalling responses of Arabidopsis to artificial root barrier systems in vitro. We demonstrate that upon encountering a barrier, reduced Arabidopsis root growth and a characteristic 'step-like' growth pattern is due to a reduction in cell elongation associated with changes in signalling gene expression. Data from RNA-sequencing combined with reporter line and mutant studies identified essential roles for reactive oxygen species, ethylene and auxin signalling during the barrier response. We propose a model in which early responses to mechanical impedance include reactive oxygen signalling integrated with ethylene and auxin responses to mediate root growth changes. Inhibition of ethylene responses allows improved growth in response to root impedance, an observation that may inform future crop breeding programmes.

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Section: Discussionmentioning

confidence: 99%

Root growth responses to mechanical impedance are regulated by a network of ROS, ethylene and auxin signalling in Arabidopsis

Jacobsen

Jervis

et al. 2021

New Phytologist

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“…Root bending has also been observed in roots grown in a medium consisting of a soft upper layer and a hard lower layer. The root exhibits a bending response at the lower, harder layer (Yamamoto et al, 2008;Yan et al, 2017Yan et al, , 2018. It has been suggested that a zone of 'mechanical weakness' is required for the bending process and that this is localised between the growing and mature zones of the root (Bizet et al, 2016).…”

Section: Discussionmentioning

confidence: 99%

Root growth responses to mechanical impedance are regulated by a network of ROS, ethylene and auxin signalling in Arabidopsis

Jacobsen

Topping

et al. 2020

Preprint

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SummaryThe growth and development of root systems, essential for plant performance, is influenced by mechanical properties of the substrate in which the plants grow. Mechanical impedance, such as by compacted soil, can reduce root elongation and limit crop productivity.To understand better the mechanisms involved in plant root responses to mechanical impedance stress, we investigated changes in the root transcriptome and hormone signalling responses of Arabidopsis to artificial root barrier systems in vitro.We demonstrate that upon encountering a barrier, reduced Arabidopsis root growth and the characteristic ‘step-like’ growth pattern is due to a reduction in cell elongation associated with changes in signalling gene expression. Data from RNA-sequencing combined with reporter line and mutant studies identified essential roles for reactive oxygen species, ethylene and auxin signalling during the barrier response.We propose a model in which early responses to mechanical impedance include reactive oxygen signalling that is followed by integrated auxin and ethylene responses to mediate root growth changes. Inhibition of ethylene responses allows improved growth in response to root impedance, a result that may inform future crop breeding programmes.

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“…In general, stiffness increases as the concentration of agar increases. In addition, high gel stiffness affects root elongation and extension [53]. The formation of sporophytes was relatively lower at higher agar concentrations.…”

Section: Discussionmentioning

confidence: 99%

Effective Propagation of Selaginella tamariscina through Optimized Medium Composition

et al. 2021

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Selaginella tamariscina is a medicinal plant that contains a variety of plant secondary metabolites; however, it is currently being collected indiscriminately from its native habitats. Hence, we have developed an efficient propagation method for S. tamariscina. Explants grown in vitro were cultured in Murashige and Skoog medium of various strengths (1/16–2x), and the highest number of sporophytes (65.7) were obtained with 1/4x MS medium. Culturing explants at various lengths (3–12 mm) for 12 weeks indicated 12 mm as the most appropriate size for sporophyte propagation. We then evaluated various concentrations of individual components, sucrose (0–5%), total nitrogen (7.5–30 mM), nitrogen ratio (3:0–0:3), and agar (0.6–0.8%), in the 1/4x MS medium for explant growth for 12 weeks. The maximum number of sporophytes were formed in media containing 3% sucrose, 15 mM nitrogen, and 0.6% agar, with a nitrogen ratio of 1:2. The propagated S. tamariscina was then acclimatized in a controlled environment to improve survival in an external environment. These results demonstrate the effective conditions for in vitro mass propagation of S. tamariscina, finding that methods utilizing sporophytes were more efficient than conventional propagation methods and yielded numerous plants in a short period.

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Resistance from agar medium impacts the helical growth of Arabidopsis primary roots

Cited by 18 publications

References 37 publications

Root growth responses to mechanical impedance are regulated by a network of ROS, ethylene and auxin signalling in Arabidopsis

Root growth responses to mechanical impedance are regulated by a network of ROS, ethylene and auxin signalling in Arabidopsis

Root growth responses to mechanical impedance are regulated by a network of ROS, ethylene and auxin signalling in Arabidopsis

Effective Propagation of Selaginella tamariscina through Optimized Medium Composition

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