Resistance Evaluation of Dominant Varieties against Southern Rice Black-Streaked Dwarf Virus in Southern China

Abstract

Southern rice black-streaked dwarf virus (SRBSDV), a Fijivirus in the Reoviridae family, is transmitted by the white-backed planthopper (Sogatella furcifera, WBPH), a long-distance migratory insect, and presents a serious threat to rice production in Asia. It was first discovered in China’s Guangdong Province in 2001 and has been endemic in the south of China and north of Vietnam for two decades, with serious outbreaks in 2009, 2010, and 2017. In this study, we evaluated the resistance of 10 dominant rice varieties from southern China, where the virus overwinters and accumulates as a source of early spring reinfection, against this virus by artificial inoculation. The results showed that in all tested varieties there was no immune resistance, but there were differences in the infection rate, with incidence rates from 21% to 90.7%, and in symptom severity, with plant weight loss from 66.71% to 91.20% and height loss from 34.1% to 65.06%. Additionally, and valuably, the virus titer and the insect vector virus acquisition potency from diseased plants were significantly different among the varieties: an over sixfold difference was determined between resistant and susceptible varieties, and there was a positive correlation between virus accumulation and insect vector virus acquisition. The results can provide a basis for the selection of rice varieties in southern China to reduce the damage of SRBSDV in this area and to minimize the reinfection source and epidemics of the virus in other rice-growing areas.

Rice varietal resistance to the vector Sogatella furcifera hinders transmission of Southern rice black‐streaked dwarf virus

“…Furthermore, it is observed that the incidence of SRBSDV disease varies greatly among rice varieties under field conditions 7 and in the laboratory, SRBSDV inoculation and acquisition differ significantly between rice varieties. 13 It can be reasoned that given the susceptibility of the majority of rice varieties to SRBSDV, varietal resistance to the vector may confer reduction in SRBSDV transmission under field conditions.…”

“…In previous studies, it has been shown that silicon amendment to rice plants 11 and sublethal concentrations of pymetrozine 12 reduce the efficiency of SRBSDV transmission by S. furcifera , which provide alternative ways to fight against the SRBSDV viral disease. Furthermore, it is observed that the incidence of SRBSDV disease varies greatly among rice varieties under field conditions 7 and in the laboratory, SRBSDV inoculation and acquisition differ significantly between rice varieties 13 . It can be reasoned that given the susceptibility of the majority of rice varieties to SRBSDV, varietal resistance to the vector may confer reduction in SRBSDV transmission under field conditions.…”

CRISPR/Cas9-mediated gene editing of vacuolar ATPase subunit d mediates phytohormone biosynthesis and virus resistance in rice

“…Although SRBSDV and RSV has been successfully controlled by international cooperation, it still exists in the majority of rice-producing areas of eastern China, with periodic outbreaks in a few rice-producing areas and the potential for additional widespread outbreaks ( Lv et al., 2017 ; Alonso et al., 2019 ). One of the most effective strategies to prevent viral diseases is growing resistant or tolerant varieties; nevertheless, almost all cultivated rice varieties are susceptible to SRBSDV ( Wang et al., 2017 ; Yu et al., 2017 ; Zhou et al., 2021 ).…”

Sublethal concentrations of pymetrozine reduce Sogatella furcifera transmission of Southern rice black‐streaked dwarf virus

“…Both WBPH nymphs and adults can transmit the virus; once infected, the vectors are viruliferous throughout their lives 6,7 . Thus far, SRBSDV resistant or tolerant rice varieties (lines) have not been available 7,8 . Therefore, the only effective way to manage the viral disease caused by SRBSDV is through vector control, 9 which, as with other viral diseases, relies on the use of insecticides 10,11 …”