CRISPR/Cas9-mediated gene editing of vacuolar ATPase subunit d mediates phytohormone biosynthesis and virus resistance in rice

Abstract

Vacuolar ATPases (V-ATPases) are proton pumps for proton translocation across membranes that utilize energy derived from ATP hydrolysis; OsV-ATPase subunit d (OsV-ATPase d) is part of an integral, membrane-embedded V0 complex in the V-ATPase complex. Whether OsV-ATPase d is involved in phytohormone biosynthesis and resistance in rice remains unknown. The knockout mutants of OsV-ATPase d in rice were generated using the CRISPR/Cas9 system, and mutation of OsV-ATPase d did not show any detrimental effect on plant growth or yield productivity. Transcriptomic results showed that OsV-ATPase d is probably involved in mediating the biosynthesis of plant hormones and resistance in rice. Compared to wild type, mutation of OsV-ATPase d significantly increased JA and ABA biosynthesis and resistance against Southern rice black-streaked dwarf virus (SRBSDV), but it decreased resistance against Rice stripe virus (RSV) in rice. The data presented in this study reveal that OsV-ATPase d mediates phytohormone biosynthesis and virus resistance in rice and can be selected as a potential target for resistance breeding in rice.

Dissecting Diagnostic and Management Strategies for Plant Viral Diseases: What Next?

“…Similarly, the editing of the translation initiation factor, the eIF4E1 gene in tomato plants, leads to enhanced resistance to infection by certain RNA viruses, including the pepper mottle virus [168]. Furthermore, the targeted modification of susceptibility (S) genes (eIF4G, Vacuolar ATPases subunit D) in rice showed enhanced resistance to multiple viruses, including Rice stripe virus [169] and Rice black-streaked dwarf virus [170]. This approach demonstrates the potential for engineering broad-spectrum resistance in various crops.…”