Resistance-breaking tomato spotted wilt orthotospovirus isolates on resistant tomato in Serbia

Petrović, Branka; Vučurović, Ana; Zečević, Katarina; Delibašić, Goran; Krstić, Branka; Stanković, Ivana

doi:10.1007/s41348-021-00493-4

Cited by 6 publications

(3 citation statements)

References 53 publications

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“…Severe TSWV-like symptoms, including stunting, leaf, stem, petiole necrosis, and concentric rings on fruits, were observed in the three sampled municipalities (Figure 1b), with disease incidence of 30%-60%. The most effective management strategy for TSWV control resides in using resistant introgressed tomato cultivars containing the Sw-5 gene; however, the intensive cultivation of resistant materials has led to the evolution of RB TSWV isolates (Petrović et al, 2021). To test the presence of Sw-5 gene-containing materials in the Baja California peninsula collected samples, genotyping was performed in 20 randomly selected samples from the three surveyed municipalities, obtaining 65% of positive plants for the corresponding resistant-associated allele (Figure S1).…”

Section: Putative Tswv Rb Isolates Infect Tomato Cultivars Containing...mentioning

confidence: 99%

“…However, the high selective pressure caused by the intensive cultivation of resistant tomato cultivars has led to the evolution of resistance‐breaking (RB) TSWV isolates in different tomato‐growing areas worldwide. Sw‐5 RB isolates have been detected in Australia (Latham & Jones, 1998), South Africa (Thompson & van Zijl, 1996), Turkey (Fidan & Sari, 2019), Italy (Ciuffo et al, 2005), Spain (Aramburu & Martí, 2003; Debreczeni et al, 2015), Serbia (Petrović et al, 2021) and the United States (Batuman et al, 2017; Cho et al, 1996). The lack of a reverse‐genetic system based on TSWV‐derived infectious clones has hampered the understanding of molecular mechanisms linked to viral evolution and resistance breakdown in tomato.…”

Section: Introductionmentioning

confidence: 99%

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A novel tomato spotted wilt virus isolate encoding a noncanonical NSm C118F substitution associated with Sw‐5 tomato gene resistance breaking

Rodríguez-Negrete

Guevara-Rivera

Arce-Leal

et al. 2023

Molecular Plant Pathology

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The nonstructural protein NSm of tomato spotted wilt virus (TSWV) has been identified as the avirulence determinant of the tomato single dominant Sw‐5 resistance gene. Although Sw‐5 effectiveness has been shown for most TSWV isolates, the emergence of resistance‐breaking (RB) isolates has been observed. It is strongly associated with two point mutations (C118Y or T120N) in the NSm viral protein. TSWV‐like symptoms were observed in tomato crop cultivars (+Sw‐5) in the Baja California peninsula, Mexico, and molecular methods confirmed the presence of TSWV. Sequence analysis of the NSm 118–120 motif and three‐dimensional protein modelling exhibited a noncanonical C118F substitution in seven isolates, suggesting that this substitution could emulate the C118Y‐related RB phenotype. Furthermore, phylogenetic and molecular analysis of the full‐length genome (TSWV‐MX) revealed its reassortment‐related evolution and confirmed that putative RB‐related features are restricted to the NSm protein. Biological and mutational NSm 118 residue assays in tomato (+Sw‐5) confirmed the RB nature of TSWV‐MX isolate, and the F118 residue plays a critical role in the RB phenotype. The discovery of a novel TSWV‐RB Mexican isolate with the presence of C118F substitution highlights a not previously described viral adaptation in the genus Orthotospovirus, and hence, the necessity of further crop monitoring to alert the establishment of novel RB isolates in cultivated tomatoes.

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Section: Putative Tswv Rb Isolates Infect Tomato Cultivars Containing...mentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

A novel tomato spotted wilt virus isolate encoding a noncanonical NSm C118F substitution associated with Sw‐5 tomato gene resistance breaking

Rodríguez-Negrete

Guevara-Rivera

Arce-Leal

et al. 2023

Molecular Plant Pathology

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“…These mutations are believed to mask this region from Sw-5b recognition while not interfering with NSm cell-to-cell movement function [ 12 ]. The C118Y (C118Y) mutation has been identified in RB isolates from many areas of the world, e.g., South Africa [ 14 ], Australia [ 15 ], Spain [ 16 ], Italy [ 17 ], United States [ 19 ], Turkey [ 20 ], and Serbia [ 21 ]. Subsequently, other aa substitution mutations have been identified in this NSm region that also allow for RB, e.g., T120N, C118F, and D122G.…”

Section: Introductionmentioning

confidence: 99%

An all-out assault on a dominant resistance gene: Local emergence, establishment, and spread of strains of tomato spotted wilt orthotospovirus (TSWV) that overcome Sw-5b-mediated resistance in fresh market and processing tomatoes in California

Macedo,

Melgarejo,

Cespedes

et al. 2024

PLoS ONE

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Tomato spotted wilt orthotospovirus (TSWV) causes substantial economic loss to tomato production, and the Sw-5b resistance gene is widely deployed for management. Here, we show (i) the emergence of resistance-breaking (RB) TSWV strains in processing and fresh market tomato production in California over the past ten years, and (ii) evolutionary relationships with RB strains from other areas. A specific RT-PCR test was used to show the C118Y RB strain that emerged in Fresno County in 2016 quickly became predominant in the central production area and remained so through this study. In 2021, the C118Y strain was detected in the Northern production area, and was predominant in 2022. However, in 2023, the C118Y strain was unexpectedly detected in fewer spotted wilt samples from resistant varieties. This was due to emergence of the T120N RB strain, previously known to occur in Spain. A specific RT-PCR test was developed and used to show that the T120N RB strain was predominant in Colusa and Sutter counties (detected in 75–80% of samples), and detected in ~50% of samples from Yolo County. Pathogenicity tests confirmed California isolates of the T120N strain infected Sw-5b tomato varieties and induced severe symptoms. Another RB strain, C118F, was associated with spotted wilt samples of Sw-5 varieties from fresh market tomato production in southern California. Phylogenetic analyses with complete NSm sequences revealed that the C118Y and T120N RB strains infecting resistant processing tomato in California emerged locally, whereas those from fresh market production were more closely related to isolates from Mexico. Thus, widespread deployment of this single dominant resistance gene in California has driven the local emergence of multiple RB strains in different tomato production areas and types. These results further emphasize the need for ongoing monitoring for RB strains, and identification of sources of resistance to these strains.

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Population analysis on tomato spotted wilt virus isolates inducing various symptoms on tomato, pepper, and Chenopodium album in Turkey

Morca¹,

Çeli̇k

Coşkan³

et al. 2022

Physiological and Molecular Plant Pathology

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Resistance-breaking tomato spotted wilt orthotospovirus isolates on resistant tomato in Serbia

Cited by 6 publications

References 53 publications

A novel tomato spotted wilt virus isolate encoding a noncanonical NSm C118F substitution associated with Sw‐5 tomato gene resistance breaking

A novel tomato spotted wilt virus isolate encoding a noncanonical NSm C118F substitution associated with Sw‐5 tomato gene resistance breaking

An all-out assault on a dominant resistance gene: Local emergence, establishment, and spread of strains of tomato spotted wilt orthotospovirus (TSWV) that overcome Sw-5b-mediated resistance in fresh market and processing tomatoes in California

Population analysis on tomato spotted wilt virus isolates inducing various symptoms on tomato, pepper, and Chenopodium album in Turkey

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