Residual risk of bacterial contamination of platelets: six years of experience with sterility testing

Ramírez‐Arcos, Sandra; DiFranco, Caesar; McIntyre, Terri; Goldman, Mindy

doi:10.1111/trf.14202

Cited by 60 publications

(83 citation statements)

References 35 publications

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“…Firstly, the use of an anaerobic culture bottle extends the bacterial spectrum that can be identified as a contamination. This benefit has been confirmed by several reports [11, 18, 39, 40]. For instance, a study from NHS Blood and Transplant showed that 66% of all confirmed positive results were only detected in anaerobic culture bottles, whereas 8% were positive in an aerobic and 26% in both bottle types [17].…”

Section: Anaerobic Screening – Is It Worth the Effort?supporting

confidence: 66%

“…This extremely low concentration is prone to sampling errors, especially when samples are collected shortly after donation when the bacterial load is far below 1 CFU/mL and knowing that bacteria tend to grow in aggregates and are not evenly distributed in solutions. Therefore, the estimated frequency of false-negative results associated with the “early sampling” strategy is alarming: only 10–50% of the contaminated blood cultures are detected as positive [18, 29]. …”

Section: Mandatory Microbiological Screening By Culture-based Methodsmentioning

confidence: 99%

“…Referring to various publications, the rate of confirmed contaminated platelets is in the range of approximately 100–2,000 per million PC [16, 18-20]. These contaminated PC are transfused if no further safety measures are in place.…”

Section: Susceptibility Of Pc To Microbial Contaminationsmentioning

confidence: 99%

“…Bacterial screening of PC using automated culture systems as any screening method is limited by sensitivity and specificity, i.e., by false-negative or false-positive results [16], leading to transfusion of contaminated or discarding of microbiologically safe PC, respectively [18]. Pathogen reduction (PR) technologies as a tool to prevent transfusion-related septic infections are, therefore, worthwhile mentioning.…”

Section: Elimination Of Bacterial Contaminations Using Pathogen Reducmentioning

confidence: 99%

“…These interventions have significantly reduced the number of patients with bacterial sepsis following transfusion in the last decade. However, a low PC percentage still displays bacterial contaminations when appropriate quality control measures are performed [16-18]. …”

Section: Susceptibility Of Pc To Microbial Contaminationsmentioning

confidence: 99%

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Microbiological Screening of Platelet Concentrates in Europe

Prax

Bekeredjian‐Ding

Krut³

2019

Transfus Med Hemother

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The risk of transfusion-associated sepsis due to transmission of bacteria is a persistent problem in the transfusion field. Despite numerous interventions to reduce the risk, cases of bacterial sepsis following transfusion are repeatedly being reported. Especially platelet concentrates are highly susceptible to bacterial contaminations due to the growth-promoting storage conditions. In Europe, blood establishments and national authorities have implemented individual precaution measures to mitigate the risk of bacterial transmission. To obtain an overview of the different approaches, we compiled information from national authorities, blood establishments, and the current literature. Several aspects such as the shelf life of platelets, time of sampling and the applied control measures are compared between the member states. The analysis of the data revealed a broad heterogeneity of procedures on a national level ranging from platelet release without any safety testing up to mandatory screening of all platelet concentrates prior to transfusion. Despite the substantial progress made in recent years, several bacterial reports on transfusion-associated sepsis indicate that further efforts are needed to increase the safety of blood transfusions in the long term.

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Section: Anaerobic Screening – Is It Worth the Effort?supporting

confidence: 66%

Section: Mandatory Microbiological Screening By Culture-based Methodsmentioning

confidence: 99%

Section: Susceptibility Of Pc To Microbial Contaminationsmentioning

confidence: 99%

Section: Elimination Of Bacterial Contaminations Using Pathogen Reducmentioning

confidence: 99%

Section: Susceptibility Of Pc To Microbial Contaminationsmentioning

confidence: 99%

See 3 more Smart Citations

Microbiological Screening of Platelet Concentrates in Europe

Prax

Bekeredjian‐Ding

Krut³

2019

Transfus Med Hemother

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Utilization of mitochondrial-targeted small molecules in protecting stored platelets against storage lesions

Zhang

Wang

Lei

et al. 2022

European Journal of Medicinal Chemistry Reports

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Transfusion-Transmitted Infections Reported to the National Healthcare Safety Network Hemovigilance Module

Haass

Sapiano

Savinkina

et al. 2019

Transfusion Medicine Reviews

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Background: Transfusion-transmitted infections (TTI) can be severe and result in death. Transfusion-transmitted viral pathogen transmission has been substantially reduced, whereas sepsis due to bacterial contamination of platelets and transfusion-transmitted babesiosis may occur more frequently. Quantifying the burden of TTI is important to develop targeted interventions. Study design and Methods:From January 1, 2010 to December 31, 2016, healthcare facilities participating in the National Healthcare Safety Network (NHSN) Hemovigilance Module (HM) monitored transfusion recipients for evidence of TTI and recorded the total number of units transfused. Facilities use standard criteria to report TTIs. Incidence rates of TTIs, including for bacterial contamination of platelets and transfusion-transmitted babesiosis are presented.Results: One hundred ninety-five facilities reported 111 TTIs and 7.9 million transfused components to the NHSN HM. Of these 111 reports, 54 met inclusion criteria. The most frequently reported pathogens were Babesia spp. in RBCs (16/23, 70%) and Staphylococcus aureus in platelets (12/30, 40%). There were 1.95 (26 apheresis, 4 whole blood derived) TTI per 100,000 transfused platelet units and 0.53 TTI per 100,000 transfused RBC components, compared to 0.68 TTI per 100,000 all transfused components. Conclusion:Bacterial contamination of platelets and transfusion-transmitted babesiosis were the most frequently reported TTIs. Interventions that reduce the burden of bacterial contamination of platelets, particularly collected by apheresis, and Babesia transmission through RBC transfusion would reduce transfusion recipient morbidity and mortality. These analyses demonstrate the value

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Residual risk of bacterial contamination of platelets: six years of experience with sterility testing

Cited by 60 publications

References 35 publications

Microbiological Screening of Platelet Concentrates in Europe

Microbiological Screening of Platelet Concentrates in Europe

Utilization of mitochondrial-targeted small molecules in protecting stored platelets against storage lesions

Transfusion-Transmitted Infections Reported to the National Healthcare Safety Network Hemovigilance Module

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