Utilization of mitochondrial-targeted small molecules in protecting stored platelets against storage lesions

Zhang, Yang; Wang, Juntao; Lei, Shuwen; Hu, Yixin; Fu, Lei

doi:10.1016/j.ejmcr.2022.100070

Cited by 4 publications

(1 citation statement)

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“…Moreover, the fission of platelet mitochondria contributes to a shift from oxidative metabolism to a more glycolytic metabolism [38]. A previous study has reported that upregulation of glycolysis and reduction in oxidative phosphorylation result in acidification of PCs during the 3 days of storage [40]. Therefore, the fission of mitochondria due to platelet activation might be involved in the acidic milieu of PCs during storage.…”

Section: Discussionmentioning

confidence: 99%

A tight interplay between platelet activation and mitochondrial DNA release promotes platelet storage lesion in platelet concentrates

Haeri,

Samiee,

Beigi

et al. 2024

Vox Sanguinis

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Background and ObjectivesPlatelet storage lesion (PSL) adversely affects the quality of platelet concentrates (PCs). Platelets are prone to activation during storage. Moreover, elevated free mitochondrial DNA (mtDNA) levels in PCs are associated with a higher risk of adverse transfusion reactions. Therefore, we aimed to evaluate the correlation between platelet activation markers and mtDNA release during PC storage.Materials and MethodsSix PCs prepared by the platelet‐rich plasma method were assessed for free mtDNA copy number using quantitative real‐time PCR and CD62P (P‐selectin) expression by flow cytometry on days 0 (PC collection day), 3, 5 and 7 of storage. Lactate dehydrogenase (LDH) activity, pH, platelet count, mean platelet volume (MPV) and platelet distribution width (PDW) were measured as well. The correlation between free mtDNA and other PSL parameters, and the correlation between all parameters, was determined.ResultsSignificant increases in free mtDNA, MPV and PDW, and a significant decrease in platelet count and pH were observed. CD62P expression and LDH activity elevated significantly, particularly on storage days 5–7 and 0–3, respectively. Moreover, a moderate positive correlation (r = 0.61) was observed between free mtDNA and CD62P expression. The r values between free mtDNA and LDH, pH, platelet count, MPV and PDW were 0.81, −0.72, −0.49, 0.81 and 0.77, respectively.ConclusionThe interplay between platelet activation and mtDNA release in promoting PSL in PCs may serve as a promising target for future research on applying additive solutions and evaluating the quality of PCs to improve transfusion and clinical outcomes.

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Section: Discussionmentioning

confidence: 99%