Residual impact of 17α-methyltestosterone and histopathological changes in sex- reversed Nile tilapia (Oreochromis niloticus)

Suseno, Dewi Nurmalita; Luqman, Epy Muhammad; Lamid, Mirni; Mukti‬, ‪Akhmad Taufiq; Suprayudi, Muhammad Agus

doi:10.4103/2305-0500.275527

Cited by 11 publications

(7 citation statements)

References 1 publication

Supporting

Mentioning

Contrasting

Order By: Relevance

“…According to the study of Suseno et al 2020, larger than normal gill lamella looked unclear between the primary and secondary lamellas which is a result of hypertrophy (cell enlargement). Furthermore, Dias-Neto et al 2016 also reported that Edema of branchial lamellae was less pronounced in the fish fed with natural feed at the end of the experiment.…”

Section: Discussionmentioning

confidence: 99%

“…Owing to the detrimental toxicity of hormone on human health which results in mostly carcinogenic and endocrine disorder and on the environment, researchers has deliberated on its use in aquaculture production (Dergal et al, 2016). Furthermore, the use of anabolic steroids has been banned because of the harmfulness to humans, fish and the environment (Suseno et al, 2020); based on the decision from the Republic of Indonesia with number KEP.52/MEN/2014 of the Ministry of Marine Affairs and Fisheries.…”

Section: Introductionmentioning

confidence: 99%

See 1 more Smart Citation

Histological changes in the gills, livers and muscles of Oreochromis niloticus fed with steriod, natural hormone and pawpaw seed based diets

Orose¹,

Wokeh²,

Odioko³

2021

EgeJFAS

View full text Add to dashboard Cite

This study was conducted to evaluate the histological changes of 17αmethyl-testosterone (MT), wet testes of bull (Bos indicus), boar (Sus domesticus), bull/mud catfish testes (B. indicus/C. gariepinus) and pawpaw (Carica papaya) seed powder-based diet on the Nile tilapia fry in an indoor experimental pond. Histological changes and efficacies in producing monosex males were determined after four months. Fifty-five fry were assigned randomly to three experimental ponds in triplicates. Five experimental feeds composed of soya-bean, fish meal, wheat bran, cassava flour were formulated by adding 0.06g of 17α- methyl-testosterone, 17.47g of bull wet testes, 25.59g of boar wet testes, 20.48g of bull/catfish and 20g of pawpaw seed powder per kg of feed to group 1, 2, 3, 4, 5 respectively. Obtained results from the morphological examination revealed a significant difference (p < 0.05) in phenotypic males of the Nile tilapia fry fed with the experimental diets. The highest indistinct percentage was recorded in the 17αmethyl-testosterone-treated diet while the lowest was recorded in diets 3 and 4 with no significant differences. All the groups showed histopathological differences in the livers, gills, and muscles. However, the liver histology of groups 1 and 5 showed central vessels that are mildly congested with abundant glycogen vacuolation and somewhat fat infiltration, while the histology of the muscles of group 5 showed pathological lesions including moderate to severe necrosis. These alterations can be considered an indication of the performance and health of fish in monosex culture medium indicating effects of plant extract and MT.

show abstract

Section: Discussionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Histological changes in the gills, livers and muscles of Oreochromis niloticus fed with steriod, natural hormone and pawpaw seed based diets

Orose¹,

Wokeh²,

Odioko³

2021

EgeJFAS

View full text Add to dashboard Cite

show abstract

“…Nile tilapia (Oreochromis sp.) is one of Indonesia's flagship export products (Suseno et al, 2020). Its aquaculture development mainly takes place in freshwater ponds, and now there is a growing trend of cultivation in brackish water ponds (salted tilapia).…”

Section: Introductionmentioning

confidence: 99%

“…Male Nile tilapia exhibit faster growth rates and larger body sizes compared to females (Bardhan et al, 2021;Kembenya & Ondiba, 2021). Cultivating male Nile tilapia alone is known as monosex tilapia farming (Suseno et al, 2020). To meet the demand for monosex culture, one approach is to provide all-male fingerlings through the process of sex reversal.…”

Section: Introductionmentioning

confidence: 99%

“…In general, sex reversal is a physiological, not genetic, process, meaning that the change in sex (gender) is purely physiological (gamete production, sperm, or oocytes) without altering the fish's genetics. Fish with female genetics can physiologically change into males and produce sperm (masculinization), while fish with male genetics can physiologically transform into females and produce oocytes or eggs (feminization) (Suseno et al, 2020). This process involves shortcuts, directly switching off one gender to the opposite gender, either by inhibiting the primary gender or inducing the phenotype of the opposite sex.…”

Section: Introductionmentioning

confidence: 99%

See 1 more Smart Citation

Temperature Incubation During the Embryo-Larval Stage for Inducing Sex Reversal of Nile Tilapia Red NIFI Strain

Soleh,

Aulia,

Naryaningsih

et al. 2023

jppipa, pendidikan ipa, fisika, biologi, kimia

View full text Add to dashboard Cite

This research aims to achieve male sex reversal in Nile Tilapia (Orechromis niloticus) of the red NIFI strain through high-temperature incubation during the embryonic or pre-swimming larval phase. Larvae resulting from the natural mating of red NIFI strain broodstock were incubated under temperature treatments of 31oC, 33oC, 35oC, and a control group (<30oC), each with three replicates. Temperature regulation was facilitated using a heater. During the initial 3 hours of incubation, the heater was turned off for 6 hours, followed by another 3 hours of incubation with the heater on. This pattern was repeated daily until the larvae began to swim. The treated larvae were then reared for 90 days. Thirty specimens from each replicate were identified for gender using the squash acetocarmine method. The study results indicated that temperature differences significantly influenced (p<0.05) the sex ratio of Nile tilapia in the red NIFI strain. The highest proportion of males was achieved at 33oC, at 73.33%, and 35oC, at 64.43%. There were no significant differences (p>0.05) in fry survival rates at 7 days and after 90 days of rearing.

show abstract

The efficacy of using pine (Pinus massoniana) pollen as an alternative to synthetic steroids in producing monosex male Nile tilapia (Oreochromis niloticus, L.)

Aziz

Mostary

Sume

et al. 2022

Aquaculture Fish & Fisheries

View full text Add to dashboard Cite

Synthetic sex steroids are widely used to produce all‐male stocks in Nile tilapia (Oreochromis niloticus). However, there is a growing concern about the use of synthetic steroid hormones in fish as food. We investigated the efficacy of a natural sex hormone as an alternative to synthetic steroids in producing monosex male tilapia. Two‐day‐old fry were fed pine pollen powder at three different concentrations (0.5%, 1.0%, 1.5%) for 28 days and subsequently reared for 3 months to observe the efficacy of pine pollen as well as to optimize the concentration for sex reversal. As a positive control, same‐aged fry of three different commercial tilapia hatcheries were fed synthetic steroid and 17‐α‐methyl testosterone‐treated feed (60 mg/kg) for the same duration. Before analyzing the phenotypic sex, the fish were reared on untreated commercial feed for 90 days in separate tanks. The 1.0% pine pollen treatment yielded a mean of 92.50 ± 0.51% males among the progeny, higher than the mean (89.49 ± 0.25%) obtained from synthetic steroid treatments. This result demonstrates that pine pollen could be an effective compound for the direct masculinization of Nile tilapia. We also obtained a significant difference (p < 0.05) in the growth performance and survival of tilapia using the dietary allotment of pine pollen. Our study suggests the feasibility of this compound as an eco‐friendly method of masculinization of tilapia and can be tried in commercial aquaculture practice.

show abstract

Residual impact of 17α-methyltestosterone and histopathological changes in sex- reversed Nile tilapia (Oreochromis niloticus)

Cited by 11 publications

References 1 publication

Histological changes in the gills, livers and muscles of Oreochromis niloticus fed with steriod, natural hormone and pawpaw seed based diets

Histological changes in the gills, livers and muscles of Oreochromis niloticus fed with steriod, natural hormone and pawpaw seed based diets

Temperature Incubation During the Embryo-Larval Stage for Inducing Sex Reversal of Nile Tilapia Red NIFI Strain

The efficacy of using pine (Pinus massoniana) pollen as an alternative to synthetic steroids in producing monosex male Nile tilapia (Oreochromis niloticus, L.)

Contact Info

Product

Resources

About