Reserve mobilization and the role of primary metabolites during the germination and initial seedling growth of rubber tree genotypes

Carvalho, Josiane Celerino de; Gonçalves, José Francisco de Carvalho; Fernandes, Andréia Varmes; Costa, Karen C. P. da; Borges, Eduardo Euclydes de Lima e; Araújo, Wagner L.; Nunes‐Nesi, Adriano; Ramos, Márcio V.; Rathinasabapathi, Bala

doi:10.1007/s11738-022-03415-5

Cited by 8 publications

(3 citation statements)

References 78 publications

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“…These authors identified ultrastructural modifications in the cotyledon epidermis correlated with haustorial function. Studies with species from other botanical families relate a similar pattern of reserve degradation through histochemical analysis (Carvalho et al, 2022;Ferreira et al, 2020;Mazzottini-dos-Santos et al, 2017;Oliveira et al, 2020) and biochemical quantifications (Carvalho et al, 2022;Mello et al, 2022;Bicalho et al, 2016). The values of starch and protein content found in this study are similar to those reported during the formation of V. reitzii seedlings (Corredor-Prado et al, 2016.…”

Section: Discussionsupporting

confidence: 81%

In vitro germination and reserve mobilization of Vriesea friburgensis Mez

2023

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Studies on the germination and establishment of plants are key pieces to understanding the reproductive success of plants. This work aimed to describe in vitro germination and reserve mobilization in the bromeliad Vriesea friburgensis through morphological, histochemical, and biochemical analysis. The conditions used in this study for the in vitro germination are adequate. From the third day of in vitro inoculation, a uniform germination of 98% was obtained, exhibiting a high physiological quality of the seeds and a high potential to produce seedlings (94%). There is early reserve mobilization, which began in the imbibition phase. The accumulated reserves in the endosperm cytoplasm are degraded by hydrolytic enzymes provided by the aleurone layer. It is possible that compounds in the cell walls of the endosperm contribute to a lesser extent in mobilization. Additionally, it was observed that starch accumulation in the cotyledon increases when the seedling has formed. Results from this study provide insights for future studies on ecology, seed technology, and conservation in this species. This study contributes to the limited knowledge of the dynamics of reserves during germination and seedling establishment in Bromeliaceae. To the best of our knowledge, this is the first study with this approach in the genus Vriesea.

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Section: Discussionsupporting

confidence: 81%

In vitro germination and reserve mobilization of Vriesea friburgensis Mez

2023

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“…During seed germination, various physiological, biochemical, and molecular changes occur [27]. One essential step is the conversion of stored starch into sugars, which serve as an energy source for the developing embryo [28]. Tis conversion is facilitated by an enzyme called α-amylase, which breaks down starch into smaller glucose molecules through hydrolysis.…”

Section: Resultsmentioning

confidence: 99%

Light-Emitting Diode Light Quality Influences Germination and Sprout Characteristics of Motherwort

Lee,

Zebro,

Heo

2023

Journal of Food Quality

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Motherwort (Leonurus japonicus Houtt) is an important medicinal plant known for its excellent antioxidant properties. Nonetheless, in South Korea, its availability remains limited due to the challenge of low seed germination rates, affecting field production. To tackle this problem, it is imperative to focus on controlled production methods. In our study, we conducted experiments in a laboratory setting, employing various combinations of light-emitting diode (LED) lights to cultivate motherwort. The influence of LED light quality on seed germination, initial growth, and functionality of motherwort is evaluated. The germination rate of motherwort ranged from 36.1 ± 7.35% to 75.0 ± 1.60%, and the highest value was observed in the red LED 100% treatment. The mean shoot length also varied depending on LED light quality. The longest shoot length (3.45 ± 0.13 g) was obtained in the red LED 100% treatment. The highest shoot weight (0.266 ± 0.011 cm) and root weight (0.051 ± 0.008 cm) were obtained in the red LED 70% and blue LED 30% mixed treatment. The total phenolic content of the motherwort sprout ranged from 2.50 ± 0.30 mg GAE/g to 3.01 ± 0.09 mg GAE/g, with the highest value from the white LED (control) treatment, but no significant differences were observed among the treatments. The red LED 30% and blue LED 70% mixed treatment showed the highest total flavonoid content (11.62 ± 0.79 mg QE/g) and DPPH radical scavenging activity (57.64 ± 2.95%). The red LED 70% and blue LED 30% mixed treatment had a similar level of DPPH scavenging activity as the control, and there was no positive or negative effect on the functionality of motherwort sprout. Overall, the results suggest that the red LED 70% and blue LED 30% mixed treatment can be effectively used to increase productivity in motherwort without decreasing its important quality.

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“…Furthermore, intrinsic factors, such as seed quality (composition of major storage components), have a notable impact on rapeseed seeds [ 12 , 13 ]; therefore, variations in quality considerably influences the resilience of seeds to stress [ 14 ]. Moreover, the mobilization of stored protein and lipids in seeds facilitates physiological metabolism during the germination process, resulting in a gradual decrease in primary reserves [ 15 ]. Protein translation ability is significantly associated with seed viability, as the inhibition of protein translation results in decreased seed germination [ 16 , 17 ].…”

Section: Introductionmentioning

confidence: 99%

Morpho-Physiochemical Indices and Transcriptome Analysis Reveal the Role of Glucosinolate and Erucic Acid in Response to Drought Stress during Seed Germination of Rapeseed

Ai,

El-Badri,

Batool

et al. 2024

IJMS

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The global expansion of rapeseed seed quality has been focused on maintaining glucosinolate (GSL) and erucic acid (EA) contents. However, the influence of seed GSL and EA contents on the germination process under drought stress remains poorly understood. Herein, 114 rapeseed accessions were divided into four groups based on GSL and EA contents to investigate their performance during seed imbibition under drought stress. Our results revealed significant variations in seed germination-related traits, particularly with higher GSL and EA, which exhibited higher germination % (G%) and lower mean germination time (MGT) under drought stress conditions. Moreover, osmoregulation, enzymatic system and hormonal regulation were improved in high GSL and high EA (HGHE) versus low GSL and low EA (LGLE) seeds, indicating the essential protective role of GSL and EA during the germination process in response to drought stress. The transcriptional regulation mechanism for coordinating GSL–EA-related pathways in response to drought stress during seed imbibition was found to involve the differential expression of sugar metabolism-, antioxidant-, and hormone-related genes with higher enrichment in HGHE compared to LGLE seeds. GO enrichment analysis showed higher variations in transcription regulator activity and DNA-binding transcription factors, as well as ATP and microtubule motor activity in GSL–EA-related pathways. Furthermore, KEGG analysis identified cellular processes, environmental information processing, and metabolism categories, with varied gene participation between GSL, EA and GSL–EA-related pathways. For further clarification, QY7 (LGLE) seeds were primed with different concentrations of GSL and EA under drought stress conditions. The results showed that 200 μmol/L of GSL and 400 μmol/L of EA significantly improved G%, MGT, and seedling fresh weight, besides regulating stress and fatty acid responsive genes during the seed germination process under drought stress conditions. Conclusively, exogenous application of GSL and EA is considered a promising method for enhancing the drought tolerance of LGLE seeds. Furthermore, the current investigation could provide a theoretical basis of GSL and EA roles and their underlying mechanisms in stress tolerance during the germination process.

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Reserve mobilization and the role of primary metabolites during the germination and initial seedling growth of rubber tree genotypes

Cited by 8 publications

References 78 publications

In vitro germination and reserve mobilization of Vriesea friburgensis Mez

In vitro germination and reserve mobilization of Vriesea friburgensis Mez

Light-Emitting Diode Light Quality Influences Germination and Sprout Characteristics of Motherwort

Morpho-Physiochemical Indices and Transcriptome Analysis Reveal the Role of Glucosinolate and Erucic Acid in Response to Drought Stress during Seed Germination of Rapeseed

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