Resequencing of Curcuma longa L. cv. Kedaram through transcriptome profiling reveals various novel transcripts

Abstract

Curcuma longa L. (Turmeric), of the family Zingiberaceae, is one of the economically as well as medicinally important plant species. It is a sterile, polyploid and vegetatively propagated spice crop cultivated usually in Southeast Asia. In the current study, we carried out re-sequencing through transcriptome profiling of Curcuma longa cv. Kedaram (Cl_Ked_6). We acquired a total of 1 GB raw data by resequencing through paired-end sequencing using Nextseq 500 platform. The raw data obtained in this study can be accessible in NCBI database with accession number of SRR3928562 with bioproject accession number PRJNA324755. Cufflinks-2.2.1 tool was used for transcriptome assembly which resulted in 39,554 numbers of transcripts. The transcript length ranged from 76 to 15,568, having N50 value of 1221 and median transcript length of 860. We annotated the transcripts using multiple databases. This data will be beneficial for studying sequence variations particularly SNPs between cultivars of turmeric towards authentic identification and discovery of novel functional transcripts in Kedaram.

OS BENEFÍCIOS MEDICINAIS DA Curcuma longa L. (AÇAFRÃO DA TERRA)

“…Dentre os seus componentes químicos ativos destaca-se a curcumina que é extraída dos rizomas da planta, empregada na indústria alimentícia (conservante, corante, tempero, alimento e suplemento dietético, inibidor de apetite e termogênico no controle da obesidade), na aromaterapia (por seus sabores e aromas atípicos de especiarias e condimentos), na indústria de cosméticos (óleos essenciais, cremes e pomadas para a pele), na indústria farmacológica (antioxidante, anticoagulante, anti-inflamatório, antitumoral, antiviral, anticancerígeno, neuroprotetor e outras fisiopatologias), na medicina tradicional (medicamento caseiro em forma de chá, decocção, maceração e infusão), na fitoterapia (utilizando-se as partes secas e frescas para extratos, chás, óleos, pomadas, comprimidos e cremes) (15,26,27) .…”

“…Sobretudo, por suas propriedades medicinais que abrangem inúmeras patologias de forma bem precisa e autêntica (3,24,25,28,29) . (4,5,6,8,9,10,11,14,15,16,17,22,25,27,28,29,30,31,32,33) .…”

Genome sequencing of turmeric provides evolutionary insights into its medicinal properties

“…Several studies have been carried out to study the secondary metabolites and medicinal properties of this plant [22], [23]. Recently, the transcriptome profiling and analysis of Curcuma longa using rhizome samples have been carried out to identify the secondary metabolite pathways and associated transcripts [11], [17], [24], [25]. However, its reference genome sequence is not yet available, which is much needed to understand the genomic and molecular basis of the evolution of the unique characteristics of Curcuma longa .…”

“…Therefore, we performed the first draft genome sequencing and assembly of Curcuma longa using 10x Genomics linked reads generated on Illumina platform. The transcriptome of rhizome tissue for this plant has been known from previous studies but the transcriptome of leaf tissue is yet unknown [11], [17], [24], [25]. Thus, we carried out the first transcriptome sequencing of leaf tissue and along with the available RNA-Seq data of rhizome, performed a comprehensive transcriptome analysis that also helped in the gene set construction.…”

Chitosan-gelatine membrane construct with different cinnamaldehyde concentration as drug delivery system in oral cavity

“…Gelatin has been extensively utilized for pharmaceutical and medical purposes. It is used as a drug and blood vessels healing [7][8][9]. In Asia countries, cinnamon was popularly used for spices in many foods.…”