Research Technologies: Fulfilling the Promise
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Angeletti, Ruth Hogue; Bonewald, Lynda F.; Jongh, Karen De; Niece, Ronald L.; Rush, A. John; Stults, John T.

doi:10.1096/fasebj.13.6.595

Cited by 15 publications

(25 citation statements)

References 4 publications

(2 reference statements)

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“…To address this challenge, members of the Association of Biomolecular Resource Facilities (ABRF) 21 designed and conducted the first phase of a large-scale ABRF-NGS Study with a focus on RNA-seq. The goals of the ABRF-NGS Study are to evaluate the performance of NGS platforms and to identify optimal methods and best practices.…”

Section: Introductionmentioning

confidence: 99%

Multi-platform assessment of transcriptome profiling using RNA-seq in the ABRF next-generation sequencing study

et al. 2014

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High-throughput RNA sequencing (RNA-seq) dramatically expands the potential for novel genomics discoveries, but the wide variety of platforms, protocols and performance has created the need for comprehensive reference data. Here we describe the Association of Biomolecular Resource Facilities next-generation sequencing (ABRF-NGS) study on RNA-seq. We tested replicate experiments across 15 laboratory sites using reference RNA standards to test four protocols (polyA-selected, ribo-depleted, size-selected and degraded) on five sequencing platforms (Illumina HiSeq, Life Technologies’ PGM and Proton, Pacific Biosciences RS and Roche’s 454). The results show high intra-platform and inter-platform concordance for expression measures across the deep-count platforms, but highly variable efficiency and cost for splice junction and variant detection between all platforms. These data also demonstrate that ribosomal RNA depletion can both enable effective analysis of degraded RNA samples and be readily compared to polyA-enriched fractions. This study provides a broad foundation for cross-platform standardization, evaluation and improvement of RNA-seq.

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Section: Introductionmentioning

confidence: 99%

Multi-platform assessment of transcriptome profiling using RNA-seq in the ABRF next-generation sequencing study

et al. 2014

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“…To be successful, Angeletti et al emphasize the importance of shared responsibilities (2). The institution must be strongly invested both financially and programmatically.…”

Section: The Effective Flow Cytometry Srl Is the Results Of Shared Insmentioning

confidence: 99%

The Flow Cytometry Shared Resource Laboratory: Best practices to assure a high‐quality, cost‐effective partnership with biomedical research laboratories

Moore

2009

Cytometry Pt A

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“…Rapid and cost‐effective realization of the projects to medical practice or intellectual property demands well‐coordinated translational support services. To provide these critical research tools, academic institutions have created core service units that promote multiuser access to research infrastructure . Core service units can be created de novo by the institution or evolve as an extension of a research program started by a single investigator.…”

Section: Introductionmentioning

confidence: 99%

“…To provide these critical research tools, academic institutions have created core service units that promote multiuser access to research infrastructure. 1 Core service units can be created de novo by the institution or evolve as an extension of a research program started by a single investigator. In both instances, the core director is oft en selected for their scientifi c accomplishments and expertise with the technology.…”

Section: Introductionmentioning

confidence: 99%

Partnership between CTSI and Business Schools Can Promote Best Practices for Core Facilities and Resources

Reeves

Dunn‐Jensen

Baldwin

et al. 2013

Clinical Translational Sci

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Biomedical research enterprises require a large number of core facilities and resources to supply the infrastructure necessary for translational research. Maintaining the financial viability and promoting efficiency in an academic environment can be particularly challenging for medical schools and universities. The Indiana Clinical and Translational Sciences Institute sought to improve core and service programs through a partnership with the Indiana University Kelley School of Business. The program paired teams of Masters of Business Administration students with cores and programs that self-identified the need for assistance in project management, financial management, marketing, or resource efficiency. The projects were developed by CTSI project managers and business school faculty using service-learning principles to ensure learning for students who also received course credit for their participation. With three years of experience, the program demonstrates a successful partnership that improves clinical research infrastructure by promoting business best practices and providing a valued learning experience for business students.

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Research Technologies: Fulfilling the Promise ¹

Cited by 15 publications

References 4 publications

Multi-platform assessment of transcriptome profiling using RNA-seq in the ABRF next-generation sequencing study

Multi-platform assessment of transcriptome profiling using RNA-seq in the ABRF next-generation sequencing study

The Flow Cytometry Shared Resource Laboratory: Best practices to assure a high‐quality, cost‐effective partnership with biomedical research laboratories

Partnership between CTSI and Business Schools Can Promote Best Practices for Core Facilities and Resources

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Research Technologies: Fulfilling the Promise 1

Cited by 15 publications

References 4 publications

Multi-platform assessment of transcriptome profiling using RNA-seq in the ABRF next-generation sequencing study

Multi-platform assessment of transcriptome profiling using RNA-seq in the ABRF next-generation sequencing study

The Flow Cytometry Shared Resource Laboratory: Best practices to assure a high‐quality, cost‐effective partnership with biomedical research laboratories

Partnership between CTSI and Business Schools Can Promote Best Practices for Core Facilities and Resources

Contact Info

Product

Resources

About

Research Technologies: Fulfilling the Promise ¹