The Flow Cytometry Shared Resource Laboratory: Best practices to assure a high‐quality, cost‐effective partnership with biomedical research laboratories

Moore, Jonni S.

doi:10.1002/cyto.a.20742

Cited by 22 publications

(34 citation statements)

References 2 publications

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“…The appointment of space for an SRL must be consistent with a reasonable ability to conduct work in a safe manner (1,30). The space should include at a minimum:…”

Section: Physical Environmentmentioning

confidence: 99%

“…A properly managed SRL involves best practices that provide necessary oversight, strategic and fiscal management/planning, self-assessments, and external reviews for evaluating the facility's performance (1,49). A properly managed SRL involves best practices that provide necessary oversight, strategic and fiscal management/planning, self-assessments, and external reviews for evaluating the facility's performance (1,49).…”

Section: Operationsmentioning

confidence: 99%

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International Society for Advancement of Cytometry (ISAC) flow cytometry shared resource laboratory (SRL) best practices

et al. 2016

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The purpose of this document is to define minimal standards for a flow cytometry shared resource laboratory (SRL) and provide guidance for best practices in several important areas. This effort is driven by the desire of International Society for the Advancement of Cytometry (ISAC) members in SRLs to define and maintain standards of excellence in flow cytometry, and act as a repository for key elements of this information (e.g. example SOPs/training material, etc.). These best practices are not intended to define specifically how to implement these recommendations, but rather to establish minimal goals for an SRL to address in order to achieve excellence. It is hoped that once these best practices are established and implemented they will serve as a template from which similar practices can be defined for other types of SRLs. Identification of the need for best practices first occurred through discussions at the CYTO 2013 SRL Forum, with the most important areas for which best practices should be defined identified through several surveys and SRL track workshops as part of CYTO 2014. © 2016 International Society for Advancement of Cytometry.

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“…The appointment of space for an SRL must be consistent with a reasonable ability to conduct work in a safe manner (1,30). The space should include at a minimum:…”

Section: Physical Environmentmentioning

confidence: 99%

Section: Operationsmentioning

confidence: 99%

International Society for Advancement of Cytometry (ISAC) flow cytometry shared resource laboratory (SRL) best practices

et al. 2016

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“…On the one hand, due to increasing knowledge of the complexity of the immune system, more subsets of leukocytes are characterized that can only be identified unequivocally by their expression pattern of a multitude of surface antigens. Compared with current methods, high-level multicolor flow cytometry offers the possibility for increased accuracy in population identification, the ability to obtain detailed information from cellular specimens, increased laboratory efficiency, and the means to consistently detect rare populations [13][14][15][16][17][18]. These characteristics are particularly well suited to the analysis of relation of the immunephenotype and cytome to other clinical parameters.…”

Section: Discussionmentioning

confidence: 97%

Practical way to develop 10-color flow cytometry protocols for the clinical laboratory

Tárnok

Bocsi

2010

SPIE Proceedings

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The latest development of commercial routine flow cytometers (FCM) is that they are equipped with three (blue, red, violet) or more lasers and many PMT detectors. Nowadays routine clinical instruments are capable of detecting 10 or more fluorescence colors simultaneously. Thereby, presenting opportunities for getting detailed information on the single cell level for cytomics and systems biology for improve diagnostics and monitoring of patients. The University Leipzig (Germany) recently started a cluster of excellence to study the molecular background of life style and environment associated diseases, enrolling 25000 individuals (LIFE). To this end the most comprehensive FCM protocol has to be developed for this study. We aimed to optimize fluorochrome and antibody combinations to the characteristics of the instrument for successful 10-color FCM. Systematic review of issues related to sampling, preparation, instrument settings, spillover and compensation matrix, reagent performance, and general principles of panel construction was performed. 10-color FCM enables for increased accuracy in cell subpopulation identification, the ability to obtain detailed information from blood specimens, improved laboratory efficiency, and the means to consistently detect major and rare cell populations. Careful attention to details of instrument and reagent performance allows for the development of panels suitable for screening of samples from healthy and diseased donors. The characteristics of this technique are particularly well suited for the analysis of broad human population cohorts and have the potential to reach the everyday practice in a standardized way for the clinical laboratory.

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“…Flow cytometry is becoming an increasingly useful tool in both health care and research laboratories because it is a rapid, accurate, and reproducible method of analysis (25). Although there is still a substantial cost for operational support in experiments involving flow cytometry, the creation of a shared resource laboratory model to enhance the scope and quality of the scientific research that applies the flow cytometry-based methodologies was recently described (26,27). In the same context, through the Oswaldo Cruz Foundation, the Brazilian government implemented the Network Technology Platforms Program for Technological Development in Health Supplies to enhance research perspectives in flow cytometry approaches, which is also suitable for diagnostic services in public health.…”

Section: Discussionmentioning

confidence: 99%

Evaluation of a Prototype Flow Cytometry Test for Serodiagnosis of Canine Visceral Leishmaniasis

Ker

Coura‐Vital

Aguiar-Soares

et al. 2013

Clin Vaccine Immunol

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g Diagnosing canine visceral leishmaniasis (CVL) is a critical challenge since conventional immunoserological tests still present some deficiencies. The current study evaluated a prototype flow cytometry serology test, using antigens and fluorescent antibodies that had been stored for 1 year at 4°C, on a broad range of serum samples. Noninfected control dogs and Leishmania infantum-infected dogs were tested, and the prototype test showed excellent performance in differentiating these groups with high sensitivity, specificity, positive and negative predictive values, and accuracy (100% in all analyses). When the CVL group was evaluated according to the dogs' clinical status, the prototype test showed outstanding accuracy in all groups with positive serology (asymptomatic II, oligosymptomatic, and symptomatic). However, in dogs which had positive results by PCR-restriction fragment length polymorphism (RFLP) but negative results by conventional serology (asymptomatic I), serological reactivity was not observed. Additionally, sera from 40 dogs immunized with different vaccines (Leishmune, Leish-Tec, or LBSap) did not present serological reactivity in the prototype test. Eighty-eight dogs infected with other pathogens (Trypanosoma cruzi, Leishmania braziliensis, Ehrlichia canis, and Babesia canis) were used to determine cross-reactivity and specificity, and the prototype test performed well, particularly in dogs infected with B. canis and E. canis (100% and 93.3% specificities, respectively). In conclusion, our data reinforce the potential of the prototype test for use as a commercial kit and highlight its outstanding performance even after storage for 1 year at 4°C. Moreover, the prototype test efficiently provided accurate CVL serodiagnosis with an absence of false-positive results in vaccinated dogs and minor cross-reactivity against other canine pathogens.

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The Flow Cytometry Shared Resource Laboratory: Best practices to assure a high‐quality, cost‐effective partnership with biomedical research laboratories

Cited by 22 publications

References 2 publications

International Society for Advancement of Cytometry (ISAC) flow cytometry shared resource laboratory (SRL) best practices

International Society for Advancement of Cytometry (ISAC) flow cytometry shared resource laboratory (SRL) best practices

Practical way to develop 10-color flow cytometry protocols for the clinical laboratory

Evaluation of a Prototype Flow Cytometry Test for Serodiagnosis of Canine Visceral Leishmaniasis

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