Research progress of organoids-on-chips in biomedical application

Wu, Qian; Pan, Yuxiang; Wan, Hao; Hu, Ning; Wang, Ping

doi:10.1360/n972018-00860

Cited by 8 publications

(6 citation statements)

References 24 publications

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“…Compared to other devices in the literature [67], [72], [77], [84], [92] the CELLviewer can perform experiments in real time in terms of physiology, toxicology, and clinical pharmacology, in addition to obtaining detailed images of current cellular morphology with resolution and high-quality data.…”

Section: Discussionmentioning

confidence: 99%

Microfluidic Live-Imaging Technology to Perform Research Activities in 3D Models

Capuzzo¹,

Vigo²

2021

Preprint

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Morphological dissimilarity and its evolution over time are one of the most unexpected variations found when comparing cell cultures in 2D and 3D. Monolayer cells appear to flatten in the lower part of the plate, adhering to and spreading in the horizontal plane while not extending vertically. Consequently, cells developed in two dimensions have a forced apex-basal polarity. Co-cultivation and crosstalking between multiple cell types, which control development and formation in the in vivo counterpart, are possible in 3D cultures. With or without a scaffold matrix, 3D model culture may exhibit more in vivo-like morphology and physiology. 3D cultures mimic relevant physiological cellular processes, transforming them into one-of-a-kind drug screening platforms. The structures and dynamics of regulatory networks, which are increasingly studied with live-imaging microscopy, must be considered to help and guarantee the functional maintenance of a 3D structure. However, commercially available technologies that can be used for current laboratory needs are minimal, despite the need to make it easier to acquire cellular kinetics with high spatial and temporal resolution, in order to improve visual efficiency and, as a result, experimentation performance. The CELLviewer is a newly developed multi-technology instrument that integrates and synchronizes the work of various scientific disciplines. The aim of this study is to test the device using two different models: a single Jurkat cell and an MCF-7 spheroid. The two models are loaded into the microfluidic cartridge for each experiment after they have been grown and captured in time-lapse for a total of 4 hours. The samples used are tracked under the operation of the optics after adaptive autofocus, while slipping inside the cartridge chamber, and the 3D rotation was successfully obtained experimentally. The MitoGreen dye, a fluorescence marker selectively permeable to live cells, was then used to determine cell viability. To measure the model diameter, construct fluorescence intensity graphs along a straight line passing through the cell, and visualize the spatial fluorescence intensity distribution in 3D, ImageJ software was used.

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Section: Discussionmentioning

confidence: 99%

Microfluidic Live-Imaging Technology to Perform Research Activities in 3D Models

Capuzzo¹,

Vigo²

2021

Preprint

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show abstract

“…Compared to other devices in the literature [67], [72], [77], [84], [92] the CELLviewer is able in addition to obtaining detailed images of current cellular morphology with resolution and highquality data; it is possible to carry out experiments in direct time in terms of: physiology, toxicology, and clinical pharmacology. The entire automated system allows full autonomy and protocol management thanks to the software making the operator free to conduct other work, thus increasing the productivity of his project.…”

Section: Discussionmentioning

confidence: 99%

Microfluidic Live-Imaging technology to perform research activities in 3D models

Capuzzo

Vigo

2021

Preprint

View full text Add to dashboard Cite

One of the most surprising differences observed when comparing cell cultures in 2D and 3D is morphological dissimilarity and their evolution over time. Cells grown in a monolayer tend to flatten in the lower part of the plate adhering to and spreading in the horizontal plane without expanding in the vertical dimension. The result is that cells grown in 2D have a forced apex-basal polarity. 3D cultures support co-cultivation and crosstalking between multiple cell types, which regulate development and formation in the in vivo counterpart. 3D models culture, with or without a scaffold matrix, can exhibit more in vivo-like morphology and physiology. 3D cultures recapitulate relevant physiological cellular processes, transforming into unique platforms for drug screening. To support and guarantee the functional maintenance of a 3D structure, one must consider the structures and dynamics of regulatory networks, increasingly studied with live-imaging microscopy. However, commercially available technologies that can be used for current laboratory needs are limited, although there is a need to facilitate the acquisition of cellular kinetics with a high spatial and temporal resolution, to elevate visual performance and consequently that of experimentation. The CELLviewer is a newly conceived and developed multi-technology instrumentation, combining and synchronizing the work of different scientific disciplines. This work aims to test the system with two models: the first model is a single Jurkat cell while the second is an MCF-7 spheroid. After having grown both models, the two models used are loaded into the microfluidic cartridge for each experiment and recorded in time-lapse for a total of 4 hours. After adaptive autofocus, when sliding inside the cartridge chamber, the samples used are tracked under the action of the optics and the 3D rotation was experimentally successfully obtained. A cell viability assessment was then used using the MitoGreen dye, a fluorescence marker selectively permeable to live cells. The ImageJ software was used to: calculate the model diameter, create fluorescence intensity graphs along a straight line passing through the cell, visualize the spatial fluorescence intensity distribution in 3D.

show abstract

“…Compared to other devices in the literature [67,72,77,84,92] the CELLviewer can perform experiments in real time in terms of physiology, toxicology, and clinical pharmacology, in addition to obtaining detailed images of current cellular morphology with resolution and high-quality data. Because of the software, the entire automated system allows for complete autonomy and protocol management, allowing the operator to focus on other tasks and increasing the project's productivity.…”

Section: Discussionmentioning

confidence: 99%

Microfluidic Live Imaging Technology to Perform Research Activities in 3D Models

Martino¹

2021

AABSc

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Morphological dissimilarity and its evolution over time are one of the most unexpected variations found when comparing cell cultures in 2D and 3D. Monolayer cells appear to flatten in the lower part of the plate, adhering to and spreading in the horizontal plane while not extending vertically. Consequently, cells developed in two dimensions have a forced apex-basal polarity. Co-cultivation and crosstalking between multiple cell types, which control development and formation in the in vivo counterpart, are possible in 3D cultures. With or without a scaffold matrix, 3D model culture may exhibit more in vivo-like morphology and physiology. 3D cultures mimic relevant physiological cellular processes, transforming them into one-of-akind drug screening platforms. The structures and dynamics of regulatory networks, which are increasingly studied with live-imaging microscopy, must be considered to help and guarantee the functional maintenance of a 3D structure. However, commercially available technologies that can be used for current laboratory needs are minimal, despite the need to make it easier to acquire cellular kinetics with high spatial and temporal resolution, in order to improve visual efficiency and, as a result, experimentation performance. The CELLviewer is a newly developed multi-technology instrument that integrates and synchronizes the work of various scientific disciplines. The aim of this study is to test the device using two different models: a single Jurkat cell and an MCF-7 1 spheroid. The two models are loaded into the microfluidic cartridge for each experiment after they have been grown and captured in time-lapse for a total of 4 hours. The samples used are tracked under the operation of the optics after adaptive autofocus, while slipping inside the cartridge chamber and the 3D rotation was successfully obtained experimentally. The MitoGreen dye, a fluorescence marker selectively permeable to live cells, was then used to determine cell viability. To measure the model diameter, construct fluorescence intensity graphs along a straight line passing through the cell, and visualize the spatial fluorescence intensity distribution in 3D, Image J software was used.

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Research progress of organoids-on-chips in biomedical application

Abstract: Progress in research and application of water mist fire suppression technology

Cited by 8 publications

References 24 publications

Microfluidic Live-Imaging Technology to Perform Research Activities in 3D Models

Microfluidic Live-Imaging Technology to Perform Research Activities in 3D Models

Microfluidic Live-Imaging technology to perform research activities in 3D models

Microfluidic Live Imaging Technology to Perform Research Activities in 3D Models

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