Research progress of CRISPR-based biosensors and bioassays for molecular diagnosis

Chen, Kun; Shen, Ziyi; Wang, Guanzhen; Zhao, Shengchao; Lin, Zihan; Liu, Wei; Cai, Yi; Mushtaq, Gohar; Jia, Jia; Wan, Chunpeng; Yan, Tingdong

doi:10.3389/fbioe.2022.986233

Cited by 10 publications

(4 citation statements)

References 142 publications

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“…In addition, the use of the CRISPR/Cas system for nucleic acid detection can achieve multiplexing and high sensitivity down to the attomolar level [ 113 ]. Therefore, the integration of CRISPR/Cas into microfluidic-based biosensors may provide accurate, highly sensitive, rapid, and powerful tools for nucleic acid detection ( Figure 8 ) [ 114 , 115 , 116 ]. A face mask with a SARS-CoV-2 sensor was developed for wearable, simple, and noninvasive detection.…”

Section: Virus Detection On Microfluidic Platformmentioning

confidence: 99%

Recent Advances in Molecular and Immunological Diagnostic Platform for Virus Detection: A Review

2023

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Severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) has caused an ongoing coronavirus disease (COVID-19) outbreak and a rising demand for the development of accurate, timely, and cost-effective diagnostic tests for SARS-CoV-2 as well as other viral infections in general. Currently, traditional virus screening methods such as plate culturing and real-time PCR are considered the gold standard with accurate and sensitive results. However, these methods still require sophisticated equipment, trained personnel, and a long analysis time. Alternatively, with the integration of microfluidic and biosensor technologies, microfluidic-based biosensors offer the ability to perform sample preparation and simultaneous detection of many analyses in one platform. High sensitivity, accuracy, portability, low cost, high throughput, and real-time detection can be achieved using a single platform. This review presents recent advances in microfluidic-based biosensors from many works to demonstrate the advantages of merging the two technologies for sensing viruses. Different platforms for virus detection are classified into two main sections: immunoassays and molecular assays. Moreover, available commercial sensing tests are analyzed.

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Section: Virus Detection On Microfluidic Platformmentioning

confidence: 99%

Recent Advances in Molecular and Immunological Diagnostic Platform for Virus Detection: A Review

2023

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“…This technique has been used for the sensing of nucleic-acid-based biomarkers of infectious and non-infectious diseases and for the detection of mutations and deletions indicative of genetic diseases. With further research, it holds promise for detecting other biomarkers such as small molecules and proteins [ 66 , 72 ]. In this section we briefly describe the application of CRISPR/Cas systems in the field of diagnostics.…”

Section: Crispr-based Diagnostics Systems: Overview and Applicationsmentioning

confidence: 99%

Potential of CRISPR/Cas system as emerging tools in the detection of viral hepatitis infection

Bahrulolum

Tarrahimofrad

Rouzbahani

et al. 2023

Virol J

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Viral hepatitis, the most common cause of inflammatory liver disease, affects hundreds of millions of people worldwide. It is most commonly associated with one of the five nominal hepatitis viruses (hepatitis A–E viruses). HBV and HCV can cause acute infections and lifelong, persistent chronic infections, while HAV and HEV cause self-limiting acute infections. HAV and HEV are predominantly transmitted through the fecal-oral route, while diseases transmitted by the other forms are blood-borne diseases. Despite the success in the treatment of viral hepatitis and the development of HAV and HBV vaccines, there is still no accurate diagnosis at the genetic level for these diseases. Timely diagnosis of viral hepatitis is a prerequisite for efficient therapeutic intervention. Due to the specificity and sensitivity of clustered regularly interspaced short palindromic repeat (CRISPR)/CRISPR-associated sequences (Cas) technology, it has the potential to meet critical needs in the field of diagnosis of viral diseases and can be used in versatile point-of-care (POC) diagnostic applications to detect viruses with both DNA and RNA genomes. In this review, we discuss recent advances in CRISPR–Cas diagnostics tools and assess their potential and prospects in rapid and effective strategies for the diagnosis and control of viral hepatitis infection.

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“…In our previous review, an increasing number of studies have shown that CRISPR/Cas technology can be integrated with biosensors and bioassays for nucleic acid detection. In addition, it is particularly important to develop drugs for clinical treatment [ 18 ]. Quercetin can interfere with SARS-CoV-2 replication theoretically as well as reduce the inflammation and toxic effects of coronavirus disease 2019 (COVID-19) vaccines [ 19 , 20 ].…”

Section: Anti-sars-cov-2 Activitymentioning

confidence: 99%

Pharmacological Activity of Quercetin: An Updated Review

Wang

Yao

et al. 2022

Evidence-Based Complementary and Alternative Medicine

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Quercetin, a natural flavonoid compound with a widespread occurrence throughout the plant kingdom, exhibits a variety of pharmacological activities. Because of the wide spectrum of health-promoting effects, quercetin has attracted much attention of dietitians and medicinal chemists. An updated review of the literature on quercetin was performed using PubMed, Embase, and Science Direct databases. This article presents an overview of recent developments in pharmacological activities of quercetin including anti-SARS-CoV-2, antioxidant, anticancer, antiaging, antiviral, and anti-inflammatory activities as well as the mechanism of actions involved. The biological activities of quercetin were evaluated both in vitro and in vivo, involving a number of cell lines and animal models, but metabolic mechanisms of quercetin in the human body are not clear. Therefore, further large sample clinical studies are needed to determine the appropriate dosage and form of quercetin for the treatment of the disease.

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Research progress of CRISPR-based biosensors and bioassays for molecular diagnosis

Cited by 10 publications

References 142 publications

Recent Advances in Molecular and Immunological Diagnostic Platform for Virus Detection: A Review

Recent Advances in Molecular and Immunological Diagnostic Platform for Virus Detection: A Review

Potential of CRISPR/Cas system as emerging tools in the detection of viral hepatitis infection

Pharmacological Activity of Quercetin: An Updated Review

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