RESEARCH PAPER Homology modeling deduced tridimensional structure of Bacillus thuringiensis Cry1Ab18 toxin

Kashyap, Sudhanshu; Singh, B D; Amla, D. V.

doi:10.5114/bta.2012.46568

Cited by 1 publication

(1 citation statement)

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“…In our study, we identified a gene encoding a delta-endotoxin CytB protein (contig 2), and hypothetical proteins that grouped in the delta-endotoxin CytB family (contigs 3 and 4). The CytB family consists of delta-endotoxin proteins, known as pore-forming toxins (PFT), which are expressed in the soil-inhabiting bacterium B. thuringiensis during sporulation and possess cytolytic and insecticidal activities against a wide range of insects (Bravo et al 2007;Kashyap et al 2012;Pardo-Lopez et al 2013). Once ingested by the host, these toxins are activated by host proteases, leading to the formation of a pore structure in the insect membrane allowing penetration of water and ions leading to cell lysis and death (Bravo et al 2007;Pigott and Ellar 2007;Soberon et al 2009).…”

Section: Carbohydrate-binding Genesmentioning

confidence: 99%

Suppression subtractive hybridization and comparative expression of a pore-forming toxin and glycosyl hydrolase genes in Rhizoctonia solani during potato sprout infection

et al. 2014

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Rhizoctonia solani is a plant pathogenic fungus that causes black scurf on tubers and stem and stolon canker on underground parts of potato plant. Early in the season, the fungus attacks germinating sprouts underground before they emerge from the soil. Damage at this stage results in delayed emergence of weakened plants with poor and uneven stands. The mechanism underlying this phenomenon has been investigated in this study by coupling a cDNA-suppression subtractive hybridization (SSH) library to differential screening to identify transcripts of R. solani that are down-regulated during infection of potato sprouts. We report on the identification of 33 unique genes with functions related to carbohydrate binding, vitamin synthesis, pathogenicity, translation, ATP and nucleic acid binding and other categories. RACE-PCR was used to clone and characterize the first full-length cDNA clones, RSENDO1 and RSGLYC1 that encode for an eukaryotic delta-endotoxin CytB protein and an intracellular glycosyl hydrolase, respectively. Quantitative real-time PCR revealed the down-regulation of RSENDO1 during infection of potato sprouts and the up-regulation of RSGLYC1 when the fungus was grown on a cellulose-based nutrient medium. In contrast, additional experiments have highlighted the down-regulation of RSENDO1 when R. solani was co-cultured with the mycoparasite Stachybotrys elegans and the bacterial antagonist Bacillus subtilis B26. These results advance our understanding of R. solani-potato interaction in subterranean parts of the plant. Such approaches could be considered in building an efficient integrated potato disease management program.

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