“…This approach has not been pursued with MD. A report of the passive transfer of resistance to MD by means of spleen cells (Feldbush and Maag, 1969) may be criticized on the grounds that the transfer involved spleen cells from donor fowls that had survived two injections of live MDV, and that therefore virus was transferred to the recipients. These experiments were more of the nature of in vivo neutralization tests than the transfer of immunity; chickens were inoculated with mixtures of MD lymphoma cells and spleen cells from the convalescent birds and a reduction in mortality caused by MD was observed, apparently related to the balance between the numbers of lymphoma cells and of spleen cells.…”
Immunization with inactivated viral antigens protected chickens against Marek's disease. Non-immunized chickens could be protected by injections of spleen cells but not of serum from immunized, histocompatible donors. Chickens rendered agamma-globulinaemic by bursectomy and irradiation could also be immunized against Marek's disease by inoculation with viral antigens, but spleen cells from these immunized, bursectomized and irradiated donors did not confer protection on the recipients into which they were injected. It was concluded that, although in the bursectomized, immunized donors cell-mediated immunity alone was able to provide a fair degree of protection against Marek's disease, the protection afforded against the disease by spleen-cell transfer was at least partly attributable to the transfer of antibody-producing cells, and that humoral immunity, while not being an absolute requirement for resistance, is normally an important component of the resistance mechanism.
“…This approach has not been pursued with MD. A report of the passive transfer of resistance to MD by means of spleen cells (Feldbush and Maag, 1969) may be criticized on the grounds that the transfer involved spleen cells from donor fowls that had survived two injections of live MDV, and that therefore virus was transferred to the recipients. These experiments were more of the nature of in vivo neutralization tests than the transfer of immunity; chickens were inoculated with mixtures of MD lymphoma cells and spleen cells from the convalescent birds and a reduction in mortality caused by MD was observed, apparently related to the balance between the numbers of lymphoma cells and of spleen cells.…”
Immunization with inactivated viral antigens protected chickens against Marek's disease. Non-immunized chickens could be protected by injections of spleen cells but not of serum from immunized, histocompatible donors. Chickens rendered agamma-globulinaemic by bursectomy and irradiation could also be immunized against Marek's disease by inoculation with viral antigens, but spleen cells from these immunized, bursectomized and irradiated donors did not confer protection on the recipients into which they were injected. It was concluded that, although in the bursectomized, immunized donors cell-mediated immunity alone was able to provide a fair degree of protection against Marek's disease, the protection afforded against the disease by spleen-cell transfer was at least partly attributable to the transfer of antibody-producing cells, and that humoral immunity, while not being an absolute requirement for resistance, is normally an important component of the resistance mechanism.
Zusammenfassung
Die experimentellen Untersuchungsergebnisse beziehen sich auf drei Themenkreise:
a) Vermehrung des Puten‐Herpesvirus in vitro: Durch Versuche in der Durchströmungskammer sowie durch Untersuchungen mit markierten Antikörpern konnte der Nachweis erbracht werden, daß die Virusweitergabe von Zelle zu Zelle durch erregerhaltige Zytoplasmateile erfolgt, die von infizierten Zellen abgeschnürt und kurze Zeit später von nicht infizierten Zellen aufgenommen werden. Unter Berücksichtigung dieses offenbar den zellassoziierten Herpesviren der Gruppe B eigenen Übertragungsweges ist eine größere Virusproduktion bei gleichzeitiger kürzerer Kultivierungszeit möglich.
b) Interferon: Während drei Puten‐Herpesvirusstämme sowie drei apathogene Hühner‐Herpesvirusstämme in vitro kein nachweisbares Interferon induzierten, waren drei geprüfte pathogene MKV‐Stämme in der Lage, sowohl in Hühnerembryofibroblasten‐ als auch in Hühnerkükennierenzellkulturen die Bildung großer Mengen Interferon zu induzieren. Auf Grund der fehlenden Interferoninduktion durch die drei Puten‐ und die drei apathogenen Hühner‐Herpesvirusstämme kann als sicher gelten, daß der Interferonmechanismus nicht für die Schutzwirkung dieser Stämme gegen das Entstehen der MK in Frage kommt.
c) Interferenz: Es konnten experimentelle Beweise für das Vorkommen der homologen Interferenz in vitro erbracht werden. Durch Endpunktpassagen mit einem zeitlichen Abstand von 24 Stunden zwischen Infektion und Passage der Kulturen konnte die Bildung inkompletter interferierender Viruspartikel weitgehend vermieden werden. Inaktiviertes Herpesvirus vermag ebenfalls mit infektiösem Virus zu interferieren.
Die Infektion von Hühnerkükennierenzellkulturen mit Puten‐Herpesvirus und die nachfolgende Infektion mit pathogenem Marekvirus vermochte die Vermehrung des MKV nachhaltig zu hemmen. Dieses in vitro beobachtete, als heterologe Interferenz bezeichnete Ereignis tritt Literaturberichten zufolge auch in vivo auf und wird als eines der Wirkungsprinzipien der Schutzimpfung von Eintagsküken gegen die MK angesehen.
Summary
Multiplication, interference and interferon induction of avian herpesviruses. Protection against Marek's Disease
2. Materials and methods, results, discussion and conclusions
The results of experimental studies relate to three different themes.
a) Multiplication of turkey herpesvirus in vitro. Studies using the continuous culture chamber and experiments with labelled antibodies have shown that spread of virus from cell to cell takes place by virus‐containing cytoplasmic particles which become detached from infected cells and are taken up by uninfected cells. Consideration of this method of transmission of herpesviruses of Group B suggests that a very large amount of virus can be produced in a very short period of culture.
b) Interferon. Whereas three turkey herpesvirus strains and three nonpathogenic chicken herpesvirus strains produced no demonstrable interferon in vitro, three pathogenic MD virus strains tested were able to induce large amounts of interferon in c...
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