Research Note: Molecular characterization of antimicrobial resistance and virulence gene analysis of Enterococcus faecalis in poultry in Tai'an, China

Yu, Liang; Yu, Lu; Liu, Mengda; Li, Zixuan; Lin, Li; Wang, Fangkun

doi:10.1016/j.psj.2022.101763

Cited by 8 publications

(9 citation statements)

References 11 publications

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“…Doxycycline is the preferred treatment for Rickettsiae and Ehrlichiae in small animals, as opposed to oxytetracycline 46 , 48 . Resistance to tetracycline in Enterococcus isolates was consistent with current results, which revealed a high resistance rate 1 , 4 , 49 , 50 , but a higher prevalence of tetracycline-resistant E. faecalis was obtained in the present study 51 – 53 . A study conducted in Tunisia between September 2011 and December 2011 showed that E. hirae species isolated from animal stools were resistant to tetracyclines, in accordance with the findings of this study 12 .…”

Section: Discussionsupporting

confidence: 92%

Species prevalence, virulence genes, and antibiotic resistance of enterococci from food-producing animals at a slaughterhouse in Turkey

Cebeci

2024

Sci Rep

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Healthy cattle, sheep, and goats can be reservoirs for gastrointestinal pathogenic fecal enterococci, some of which could be multidrug-resistant to antimicrobials. The objective of this study was to determine the prevalence and diversity of Enterococcus species in healthy sheep, goat, and cattle carcasses, as well as to analyze the antimicrobial resistance phenotype/genotype and the virulence gene content. During 2019–2020, carcass surface samples were collected from 150 ruminants in a slaughterhouse. A total of 90 enterococci, comprising five species, were obtained. The overall prevalence of enterococci was found to be 60%, out of which 37.7% were identified as Enterococcus (E.) hirae, 33.3% as E. casseliflavus, 15.5% as E. faecium, 12.2% as E. faecalis, and 1.1% as E. gallinarum. Virulence-associated genes of efaA (12.2%) were commonly observed in the Enterococcus isolates, followed by gelE (3.3%), asaI (3.3%), and ace (2.2%). High resistance to quinupristin-dalfopristin (28.8%), tetracycline (21.1%), ampicillin (20%), and rifampin (15.5%) was found in two, four, four, and five of the Enterococcus species group, respectively. The resistance of Enterococcus isolates to 11 antibiotic groups was determined and multidrug resistant (MDR) strains were found in 18.8% of Enterococcus isolates. Characteristic resistance genes were identified by PCR with an incidence of 6.6%, 2.2%, 1.1%, 1.1%, 1.1%, and 1.1% for the tetM, ermB, ermA, aac(6ʹ)Ie-aph(2")-la, VanC1, and VanC2 genes in Enterococcus isolates, respectively. Efflux pump genes causing multidrug resistance were detected in Enterococcus isolates (34.4%). The results showed that there were enterococci in the slaughterhouse with a number of genes linked to virulence that could be harmful to human health.

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Section: Discussionsupporting

confidence: 92%

Species prevalence, virulence genes, and antibiotic resistance of enterococci from food-producing animals at a slaughterhouse in Turkey

Cebeci

2024

Sci Rep

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“…Due to the lack of effective vaccines and other green prevention and control technologies, food-borne zoonotic bacterial diseases are usually treated with antibiotics. However, excessive and/or improper use of antibiotics in livestock and poultry has led to an increase in antimicrobial-resistant and MDR bacteria worldwide, becoming a global concern for human and animal health ( Li et al, 2020 , Wang et al., 2021 , Yu et al., 2022 ). In this study, MDR K. pneumoniae was a serious phenomenon, as the bacteria showed a MDR rate of 87.88%—higher than the 22% MDR prevalence rate in Hong Kong, China, similar to the 85% rate in India ( Poudel et al, 2019 ), and lower than the 96.67% rate in a commercial broiler slaughter factory in Shandong ( Wu et al, 2016 ).…”

Section: Discussionmentioning

confidence: 99%

Prevalence and antimicrobial susceptibility profiles of ESBL-producing Klebsiella Pneumoniae from broiler chicken farms in Shandong Province, China

Li¹,

Li²,

Peng³

et al. 2022

Poultry Science

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“…According to Na′s report, 35.9% of Enterococcus isolates from duck feces and carcasses from four slaughterhouses in southern Korea were MDR, 2.3% of which harbor the optrA gene (Na et al, 2019). Yu et al reported that 72.13% of Enterococcus isolates from broilers slaughterhouses in Tai'an, China, were MDR, with 18.03% being linezolid-resistant (Yu et al, 2022a). Notably, a vancomycin-resistant E. faecium was isolated from a transport crate in a chicken slaughterhouse in Germany (Savin et al, 2020).…”

Section: Introductionmentioning

confidence: 99%

Transmission of linezolid-resistant Enterococcus isolates carrying optrA and poxtA genes in slaughterhouses

Long

Wang

et al. 2023

Front. Sustain. Food Syst.

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IntroductionThe presence of linezolid-resistant enterococci found in animal-derived food has attracted attention for possible transmission to human-derived enterococci through the food chain. Linezolid-resistant enterococci in farms have been widely reported, but enterococci carrying antimicrobial resistance (AMR) genes poxtA, optrA, or cfr(D) in slaughterhouse environments have not been well addressed.MethodsEnterococcus was isolated from the samples collected from two slaughterhouses in Hangzhou, and the Enterococcus carrying linezolidin-resistant genes was identified by PCR. The minimum inhibitory concentration (MIC) of the Enterococcus carrying linezolidin-resistant genes was determined by microbroth dilution method. Finally, the whole genome of strains carrying two or more linezoline resistance genes was sequenced using the Oxford Nanopore TechnologyResultsHere, 291 enterococci strains were isolated from 309 samples (94.17%). A total of 4 poxtA-positive enterococci and 42 optrA-positive enterococci were identified based on PCR. The antimicrobial susceptibility test showed that the highest rate of florfenicol resistance was 97.82% and the rate of multidrug resistance (MDR) was 95.65%. Two strains carried multiple linezolid resistance genes, among which Enterococcus casseliflavus CQFYY22-063 cocarrying optrA, poxtA, and cfr(D) was isolated from the duck cecum, and Enterococcus faecium CQFYH22-006 cocarrying optrA and poxtA was isolated from slaughterhouse sewage for the first time. Furthermore, Oxford Nanopore Technology revealed that the optrA gene of strain CQFYY22-063 was located on the Inc18-type plasmid pFYY063-optrA-70K, and the poxtA and cfr(D) genes were located on the Inc18-type plasmid pFYY063- poxtA-12K. Meanwhile, the poxtA gene of strain CQFYH22-006 was located on the Rep3-type plasmid pFYH006-poxtA-25K, and the optrA gene was located on the chromosome.DiscussionTogether, linezolid resistance in slaughterhouses deserves extensive attention, indicating the need to strengthen the monitoring of different links in the food production chain within the One Health concept.

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Research Note: Molecular characterization of antimicrobial resistance and virulence gene analysis of Enterococcus faecalis in poultry in Tai'an, China

Cited by 8 publications

References 11 publications

Species prevalence, virulence genes, and antibiotic resistance of enterococci from food-producing animals at a slaughterhouse in Turkey

Species prevalence, virulence genes, and antibiotic resistance of enterococci from food-producing animals at a slaughterhouse in Turkey

Prevalence and antimicrobial susceptibility profiles of ESBL-producing Klebsiella Pneumoniae from broiler chicken farms in Shandong Province, China

Transmission of linezolid-resistant Enterococcus isolates carrying optrA and poxtA genes in slaughterhouses

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