Research Article Genetic diversity of a native population of <em>Myrcia ovata</em> (Myrtaceae) using ISSR molecular markers

Abstract

Myrcia ovata Cambess. (Myrtaceae) is a medicinal and aromatic plant that has analgesic, bactericidal and fungicidal properties. Even though this plant has economic potential, nothing is known about the variability and genetic diversity of this species. This information is necessary to establish conservation strategies and allow prospection of natural resources. The aim of this study was to evaluate the genetic diversity of M. ovata individuals of a native population in the municipality of Japaratuba, Sergipe State, Brazil, using Inter-Simple Sequence Repeat molecular markers (ISSR). Nine primers were tested, resulting in 99 polymorphic bands. The 24 individuals evaluated were clustered in two groups by the software Structure. The Jaccard similarity ranged from 0.21 (MYRO-034 and MYRO-159) to 0.82 (MYRO-178.1 and MYRO-178.2), with an average of 0.38. The genetic diversity of M. ovata was considered of medium level. The individuals MYRO-154, MYRO-175 and MYRO-175.1 presented the most variability.

Assessing the genetic diversity of Myrsine umbellata (Primulaceae) in Brazilian Atlantic Forest remnants - an important step towards reforestation efforts

“…Inter Simple Sequence Repeat (ISSR) is a DNA marker widely used to estimate the genetic diversity of natural plant populations (e.g., Sheng et al 2017;White et al 2018). This marker was previously applied to study the genetic diversity of M. coriacea (Sw.) R. Br.…”

Genetic structure and diversity of Calycolpus moritzianus (Myrtaceae) in the north-eastern Andes of Colombia

“…Nevertheless, because morphological characters are highly influenced by the environment (phenotypic plasticity) and plant phenology (Arnold et al, 2019;Gianoli & Valladares, 2012), molecular characterizations and the use of DNA markers, which is environmentally independent, are an alternative approach to assess plant genetic variation (Alfalahi et al, 2019;Ansari et al, 2015). The genetic diversity of some Myrtaceae species in South America has been evaluated by using restriction fragment length polymorphisms (AFLP) in Acca sellowiana (Quezada et al, 2014), randomly amplified polymorphic DNA (RAPD) in Eugenia dysenterica (Trindade & Chaves, 2005), and Psidium guajava (Valera-Montero et al, 2016), inter simple sequence repeat (ISSR) in Myrcia lundiana (Alves et al, 2016) and Myrcia ovata (White et al, 2018), and the random amplified microsatellites (RAMs) in Psidium guajava (Sanabria et al, 2006). RAMs molecular markers, are a PCR-based method which uses primers containing microsatellite sequences and degenerate anchors at the 5' end (Hantula et al, 1996;Zietkiewicz et al, 1994).…”