Research Article Genetic diversity in natural populations of &lt;i&gt;Stylosanthes&lt;/i&gt; &lt;i&gt;scabra&lt;/i&gt; Fabaceae using ISSR markers

Costa, Jean; Fracetto, Giselle Gomes Monteiro; Fracetto, Felipe José Cury; Souza, Thadeu Carlos de; Santos, Mércia Virgínia Ferreira dos; Lira, M. A.

doi:10.4238/gmr18219

Cited by 2 publications

(2 citation statements)

References 33 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…The ISSR technique is known to be sensitive enough to detect low levels of genetic diversity while still having the benefits of ease and low cost. It can be used to detect the genetic diversity in Vicia, chickpea, Katsouni pea and Stylosanthes scabra (Sarwat, 2012;Singh et al, 2014;Laureles et al, 2015;Rajkovic′ et al, 2015;Costa et al, 2019;Stavridou et al, 2020) For more exact conclusions, DNA sequencing can be used as sequences are deemed to be largely stable throughout evolution and speciation. One sequence, that is, the internal transcribed spacer (ITS) region of ribosomal DNA, has provided promising results for phylogenetic analyses (Mishra et al, 2016).…”

Section: Introductionmentioning

confidence: 99%

Characterization of the Genetic Diversity of Some Species of Genus Vicia Using ISSR and ITS Molecular Techniques

et al. 2022

View full text Add to dashboard Cite

T HE HIGH economic importance of the genus Vicia has led to a large body of studies on the molecular characterization and investigation of phylogenetic relationships among species that belonged to this genus. The Mediterranean area is the principal center of distribution and diversification where polymorphism has been associated with the geographical origin of germplasm (ICARDA, 2013). The extent of genetic diversity in germplasm can be assessed through morphological characterization and genetic markers. The molecular techniques have a more accurate evaluation of the genetic and environmental components of variation, bringing greater precision to measures of genetic diversity. The present study is a molecular analysis of Vicia germplasm through ISSR and ITS techniques. The objectives were to determine the genetic diversity and phylogenetic relationship among defined 19 accessions, collected by ICARDA from different countries and habitats, representing nine Vicia species and subspecies: V. ervilia; V. monantha; V. villosa subsp. villosa, V. villosa subsp. dasycarpa and V. villosa subsp. eriocarpa; V. sativa subsp. nigra, subsp. amphicarpa, subsp. macrocacrpa and subsp. sativa. The patterns of variation of those accessions were studied by the numerical analysis of data to generate pertinent genetic information for the complement of passport data of ICARDA germplasm collections.

show abstract

Section: Introductionmentioning

confidence: 99%

Characterization of the Genetic Diversity of Some Species of Genus Vicia Using ISSR and ITS Molecular Techniques

et al. 2022

View full text Add to dashboard Cite

show abstract

“…The genome of S. scabra , in turn, has been studied primarily using cytogenetic strategies (through methods such as fluorescence in situ hybridization (FISH) and genomic in situ hybridization (GISH) [ 18 ]) or molecular markers (ISSR markers, for example [ 19 ]). Marques et al [ 18 ] also focused on sequencing and comparing plastomes (chloroplast genome, specifically) and rDNA (ribosomal DNA) sequences between different species of the genus Stylosanthes (including S. scabra ), performing an evolutionary analysis.…”

Section: Introductionmentioning

confidence: 99%

Bridging the Gap: Combining Genomics and Transcriptomics Approaches to Understand Stylosanthes scabra, an Orphan Legume from the Brazilian Caatinga

Ferreira-Neto,

da Silva,

Binneck

et al. 2023

Plants

View full text Add to dashboard Cite

Stylosanthes scabra is a scientifically orphaned legume found in the Brazilian Caatinga biome (a semi-arid environment). This work utilized omics approaches to investigate some ecophysiological aspects of stress tolerance/resistance in S. scabra, study its genomic landscape, and predict potential metabolic pathways. Considering its high-confidence conceptual proteome, 1694 (~2.6%) proteins were associated with resistance proteins, some of which were found in soybean QTL regions that confer resistance to Asian soybean rust. S. scabra was also found to be a potential source of terpenes, as biosynthetic gene clusters associated with terpene biosynthesis were identified in its genome. The analysis revealed that mobile elements comprised approximately 59% of the sequenced genome. In the remaining 41% of the sections, some of the 22,681 protein-coding gene families were categorized into two informational groups: those that were specific to S. scabra and those that expanded significantly compared to their immediate ancestor. Biological process enrichment analyses indicated that these gene families play fundamental roles in the adaptation of S. scabra to extreme environments. Additionally, phylogenomic analysis indicated a close evolutionary relationship between the genera Stylosanthes and Arachis. Finally, this study found a high number (57) of aquaporin-encoding loci in the S. scabra genome. RNA-Seq and qPCR data suggested that the PIP subfamily may play a key role in the species’ adaptation to water deficit conditions. Overall, these results provide valuable insights into S. scabra biology and a wealth of gene/transcript information for future legume omics studies.

show abstract

Research Article Genetic diversity in natural populations of <i>Stylosanthes</i> <i>scabra</i> Fabaceae using ISSR markers

Abstract: than within them. Overall, the population variability found will be useful for breeding programs for this species.

Cited by 2 publications

References 33 publications

Characterization of the Genetic Diversity of Some Species of Genus Vicia Using ISSR and ITS Molecular Techniques

Characterization of the Genetic Diversity of Some Species of Genus Vicia Using ISSR and ITS Molecular Techniques

Bridging the Gap: Combining Genomics and Transcriptomics Approaches to Understand Stylosanthes scabra, an Orphan Legume from the Brazilian Caatinga

Contact Info

Product

Resources

About