Characterization of the Genetic Diversity of Some Species of Genus Vicia Using ISSR and ITS Molecular Techniques

Abstract

T HE HIGH economic importance of the genus Vicia has led to a large body of studies on the molecular characterization and investigation of phylogenetic relationships among species that belonged to this genus. The Mediterranean area is the principal center of distribution and diversification where polymorphism has been associated with the geographical origin of germplasm (ICARDA, 2013). The extent of genetic diversity in germplasm can be assessed through morphological characterization and genetic markers. The molecular techniques have a more accurate evaluation of the genetic and environmental components of variation, bringing greater precision to measures of genetic diversity. The present study is a molecular analysis of Vicia germplasm through ISSR and ITS techniques. The objectives were to determine the genetic diversity and phylogenetic relationship among defined 19 accessions, collected by ICARDA from different countries and habitats, representing nine Vicia species and subspecies: V. ervilia; V. monantha; V. villosa subsp. villosa, V. villosa subsp. dasycarpa and V. villosa subsp. eriocarpa; V. sativa subsp. nigra, subsp. amphicarpa, subsp. macrocacrpa and subsp. sativa. The patterns of variation of those accessions were studied by the numerical analysis of data to generate pertinent genetic information for the complement of passport data of ICARDA germplasm collections.

Comparative Analysis of the Genetic Diversity of Faba Bean (Vicia faba L.)

“…Furthermore, regarding the Shannon diversity index, SCoT analysis revealed an average of 0.220, while in other studies, the average was 0.63 for SSR markers [42], 0.41 for ISSR markers [26] and 0.29 for RAPD markers [43]. The results of our study are really close to the results reported by [47]. They studied the genetic diversity of some species of genus Vicia using ISSR and ITS molecular techniques.…”

EMS-mutagenesis, In vitro Selection for Drought (PEG) Tolerance and Molecular Characterization of Mutants in Rice (Oryza sativa L.) Employing qRT-PCR and ISSR Markers

“…Many publications have successfully used the ISSR technique to confirm genetic diversity and discover somaclonal variations of many plant species, including tea (Devarumath et al, 2002), date palm (Ahmed et al, 2012), Picorriza kurroa (Rawat et al, 2013), Artemisia absinthium (Kour et al, 2014), sugarcane (Thorat et al, 2017;Shingote et al, 2019) and Vicia (El-Badan et al, 2022). ISSR has also been used to detect polymorphism produced by EMS in tissue culture grown plants (Perera et al, 2015).…”