Research Article Antioxidant activity and chemical composition of oleoresin from leaves and flowers of Brunfelsia uniflora.

Abstract

ABSTRACT. In this study, the temperature and pressure of supercritical CO 2 extraction were evaluated to obtain oleoresin of Brunfelsia uniflora leaves and flowers. The oleoresin compounds were identified by gas chromatography-mass spectrometry. The antioxidant activity was evaluated by three different methods. The highest oleoresin yields were 3.32% at 40°C and 200 bar for the leaves, and 1.03% at 60°C and 200 bar for the flowers. The main extracted compounds from leaves were phytol varying from 11.95 to 36.42% and α-tocopherol from 15.53 to 43.10%, and from flowers were geranyl linalool from 11.05 to 21.42% and α-amyrin from 9.66 to 22.12%. Oleoresin obtained at 60°C and 150 bar from leaves presented high antioxidant activity by DPPH (IC 50 1.90 mg/mL) and by FRAP (1.8 µmol Fe 2+ /mg). β-carotene/linoleic acid co-oxidation oleoresin from leaves at 0.25 mg/mL presented higher antioxidant activity than Trolox. The total phenolic content of the oleoresin from leaves ranged from 66.20 to 83.33 µg/mg and from flowers it was just up to 12.46 µg/mg. The extraction conditions affected yield, chemical composition, and antioxidant activity of oleoresin from leaves and flowers. This is the first report on the antioxidant activity of B. uniflora oleoresin from leaves and flowers and provides subsidies for potential applications in chemical, pharmaceutical, and food industries.

Antimicrobial activity and chemical composition of Brunfelsia uniflora flower oleoresin extracted by supercritical carbon dioxide

“…Studies carried out by our research group show that the yield of B. uniflora flower oleoresin obtained by supercritical carbon dioxide varies from 0.83 to 1.03%. However, according to Jorge (2016) the essential yield oil of B. uniflora by hydrodistillation is close to zero.…”

“…Jorge (2016) reported that the pressure increase from 150 to 200 bars of supercritical carbon dioxide increases the extraction of geranyllinalool from 11.72 to 21.52% in oleoresin. Zrira et al (2008) reported that extraction yield of A. visnaga oleoresin was greater (1.5%) by supercritical carbon dioxide when compared to hydrodistillation (0.4%).…”

Assessment of the Antimicrobial, Antioxidant, and Antiproliferative Potential of Sideritis raeseri subps. raeseri Essential Oil

“…However, similar activity was also observed against the non-carcinoma cell line HaCaT, indicating that it may not possess cancer-specific antiproliferative activity, although further studies are required. Although main components of the EO have been reported as main constituents in other plant extracts with similar biological activities [ 43 , 44 , 45 ], there are no available studies on the biological activities of the individual compounds, which is a valid area of further investigation. Overall, the results of our study indicated that EO form S. raeseri subsp.…”

Determination of a Chemical Marker in Dipterocarpus alatus Oleoresin Samples and Bioactivity Screening via Antioxidants, Nitric Oxide Inhibition on Murine RAW 264.7 Cells, and Collagen Production on Normal Human Dermal Fibroblasts

“…The FRAP reagent was prepared fresh daily by mixing of 25 mL acetate buffer, 2.5 mL of 10 mM TPTZ solution, and 2.5 mL of 20 mM FeCl3 6H2O solution and warmed to 37°C in a water bath until used for the experiments. 17 The 50 µL of 250 µg/mL D. alatus oleoresin samples (OD1-OD5), Trolox or 100 µg/mL of standard (-)-ISSN 2616-0692 (Electronic) 852 © 2021 the authors. This work is licensed under the Creative Commons Attribution 4.0 International License α-gurjunene in methanol were mixed with 150 µL of the FRAP reagent for 30 min in the dark condition.…”