Research and Utilization of Active Ingredients of Angelicae pubescentis

冯, 彦梅

doi:10.12677/tcm.2018.76059

Cited by 3 publications

(2 citation statements)

References 13 publications

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“…Besides its medicinal use, Duhuo has been applied in plant protection and cosmetics. Some traditional Chinese wines and teas are made using Duhuo as the main raw material (Feng et al, 2018). Alternatively, Duhuo is used to produce plant-based pesticides because its ethanol extract has significant inhibitory effects on some bacteria (Feng et al, 2018).…”

Section: Introductionmentioning

confidence: 99%

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Section: Introductionmentioning

confidence: 99%

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Section: Introductionmentioning

confidence: 99%

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Constructing a Core Collection of the Medicinal Plant Angelica biserrata Using Genetic and Metabolic Data

Liu

Wang

et al. 2020

Front. Plant Sci.

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Angelica biserrata is an important medicinal plant in Chinese traditional medicine. Its roots, which are known as Duhuo in Chinese, are broadly applied to treat inflammation, arthritis, and headache. With increasing market demand, the wild resources of A. biserrata have been overexploited, and conservation, assessment of genetic resources and breeding for this species is needed. Here, we sequenced the transcriptome of A. biserrata and developed simple sequence repeat (SSR) markers from it to construct a core collection based on 208 samples collected from Changyang-related regions. A total of 132 alleles were obtained for 17 SSR loci used with the polymorphic information content (PIC) ranging from 0.44 to 0.83. Abundant genetic diversity was inferred by Shannon’s information index (1.51), observed (0.57) and expected heterozygosity (0.72). The clustering analysis resulted into two sample groups and analysis of molecular variance (AMOVA) showed only 6% genetic variation existed among populations. A further metabolic analysis of these samples revealed the main coumarin contents, such as osthole and columbianadin. According to the genetic and metabolic data, we adopted the least distance stepwise sampling strategy to construct seven preliminary core collections, of which the 20CC collection, which possessed 42 A. biserrata individuals accounting for 90.20% of the genetic diversity of the original germplasm, represented the best core collection. This study will contribute to the conservation and management of A. biserrata wild germplasm resources and provide a material basis for future selection and breeding of this medicinal plant.

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Research and Utilization of Active Ingredients of Angelicae pubescentis

Cited by 3 publications

References 13 publications

Image_1.TIF

Image_1.TIF

Table_13.xlsx

Constructing a Core Collection of the Medicinal Plant Angelica biserrata Using Genetic and Metabolic Data

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